216 research outputs found
Building the American Republic, Volume 2
No full textBuilding the American Republic combines centuries of perspectives and voices into a fluid narrative of the United States. Throughout their respective volumes, Harry L. Watson and Jane Dailey take care to integrate varied scholarly perspectives and work to engage a diverse readership by addressing what we all share: membership in a democratic republic, with joint claims on its self-governing tradition. It will be one of the first peer-reviewed American history textbooks to be offered completely free in digital form. Visit buildingtheamericanrepublic.org for more information. The American nation came apart in a violent civil war less than a century after ratification of the Constitution. When it was reborn five years later, both the republic and its Constitution were transformed. Volume 2 opens as America struggles to regain its footing, reeling from a presidential assassination and facing massive economic growth, rapid demographic change, and combustive politics. The next century and a half saw the United States enter and then dominate the world stage, even as the country struggled to live up to its own principles of liberty, justice, and equality. Volume 2 of Building the American Republic takes the reader from the Gilded Age to the present, as the nation becomes an imperial power, rethinks the Constitution, witnesses the rise of powerful new technologies, and navigates an always-shifting cultural landscape shaped by an increasingly diverse population. Ending with the 2016 election, this volume provides a needed reminder that the future of the American republic depends on a citizenry that understands—and can learn from—its history
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Building the American Republic, Volume 2
No full textBuilding the American Republic combines centuries of perspectives and voices into a fluid narrative of the United States. Throughout their respective volumes, Harry L. Watson and Jane Dailey take care to integrate varied scholarly perspectives and work to engage a diverse readership by addressing what we all share: membership in a democratic republic, with joint claims on its self-governing tradition. It will be one of the first peer-reviewed American history textbooks to be offered completely free in digital form. Visit buildingtheamericanrepublic.org for more information. The American nation came apart in a violent civil war less than a century after ratification of the Constitution. When it was reborn five years later, both the republic and its Constitution were transformed. Volume 2 opens as America struggles to regain its footing, reeling from a presidential assassination and facing massive economic growth, rapid demographic change, and combustive politics. The next century and a half saw the United States enter and then dominate the world stage, even as the country struggled to live up to its own principles of liberty, justice, and equality. Volume 2 of Building the American Republic takes the reader from the Gilded Age to the present, as the nation becomes an imperial power, rethinks the Constitution, witnesses the rise of powerful new technologies, and navigates an always-shifting cultural landscape shaped by an increasingly diverse population. Ending with the 2016 election, this volume provides a needed reminder that the future of the American republic depends on a citizenry that understands—and can learn from—its history
[Photograph 2012.201.B0152.0701]
No full textPhotograph used for a story in the Daily Oklahoman newspaper. Caption: "Author Janet Dailey and her husband, Bill Dailey, chat with Irving Stone, whose latest book is about Charles Darwin.
A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation.
No full textA continuous spectrofluorimetric assay for protoporphyrinogen oxidase (PPO, EC 1.3.3.4) activity has been developed using a 96-well plate reader. Protoporphyrinogen IX, the tetrapyrrole substrate, is a colorless nonfluorescent compound. The evolution of the fluorescent tetrapyrrole product, protoporphyrin IX, was detected using a fluorescence plate reader. The apparent Km (Kapp) values for protoporphyrinogen IX were measured as 3.8+/-0.3, 3.6+/-0.5, and 1.0+/-0.1 microM for the enzymes from human, Myxococcus xanthus, and Aquifex aeolicus, respectively. The Ki for acifluorfen, a diphenylether herbicide, was measured as 0.53 microM for the human enzyme. Also, the specific activity of mouse liver mitochondrial PPO was measured as 0.043 nmol h-1/mg mitochondria, demonstrating that this technique is useful for monitoring low-enzyme activities. This method can be used to accurately measure activities as low as 0.5 nM min-1, representing a 50-fold increase in sensitivity over the currently used discontinuous assay. Furthermore, this continuous assay may be used to monitor up to 96 samples simultaneously. These obvious advantages over the discontinuous assay will be of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples
Identification of [2Fe-2S] clusters in microbial ferrochelatases
No full textThis article cites 20 articles, 6 of which can be accessed free at
Human protoporphyrinogen oxidase: Expression, purification, and characterization of the cloned enzyme
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