4,745 research outputs found
Characterization of microarray hybridization stoichiometry
No full textStoichiometry or reaction stoichiometry is de ned as the calculation of quantitative relationships of reactants in a given reaction. Over the last decade microarray-based assays have evolved from a nascent technology into a key molecular tool widely used in basic, translational, and clinical research [1]. Their primary utility has been gene expression analyses, facilitating high-throughput transcriptome pro ling [2]. Microarrays have also found utility in genotyping and re-sequencing applications, comparative genomic hybridization, and genome-wide (epigenetic) localization studies [3-6]. Regardless of the platform, a microarray is comprised of a library of nucleic acid sequences immobilized in a grid on a solid surface. The nucleotide sequence contained in each unique microarray feature is termed a “probe” and is derived from a speci c gene. The probe detects a speci c labeled target sequence derived from cellular messenger ribonucleic acid (mRNA) via complementary base pair hybridization.</p
Michael Rodriguez interviews author Gary Gildner
No full textAuthor Gary Gildner explains why he left his tenured teaching position to move to Idaho to became a full-time writer of poetry. Gildner talks about donating his personal papers to Michigan State University Libraries' Special Collections, his writing style and how he approaches writing. Gildner is interviewed by MSU Librarian Michael Rodriguez for the MSU Libraries' Michigan Writer Series. Held at the MSU Main Library
THE INFINITI™ SYSTEMAN AUTOMATED MULTIPLEXING MICROARRAY PLATFORM FOR CLINICAL LABORATORIES
No full textAuthor Gary Gildner reads his selected works at the Michigan Writers Series
No full textAuthor Gary Gildner reads "Sleepy time gal," "Pavol Hudak, the poet, is talking," and "Genealogy" then answers questions from the audience. The event is convened by Peter Berg, head of the Michigan State University Libraries' Special Collections. Part of the MSU Libraries' Michigan Writers Series. Held at the MSU Main Library
Microarrays in neuroscience
No full textGene expression analysis has progressed rapidly over the past decade from the historical analyses of individual genes to the sophisticated genome surveys routinely performed today. DNA biochips have facilitated this progression and helped accelerate target validation and drug discovery efforts by the pharmaceutical industry. The increasing use and acceptance of biochips to study genetic and cellular processes is clearly demonstrated by the large number of publications in recent years and the emergence of several robust commercial microarray platforms (1-3). Affymetrix (Santa Clara, Ca) pioneered this field and has been the market leader for many years, applying photolithographic technologies derived from the semiconductor industry to the fabrication of high-density 25-mer oligonucleotide microarrays. Alternative commercial platforms equally as robust have emerged including array platforms from Agilent, Amersham, Illumina and Applied Biosystems (see Chapter 1 of this book for further discussion of the technology platforms)
Dress, law and naked truth : a cultural study of fashion and form
No full textWhy are civil authorities in so-called liberal democracies affronted by public nudity and the Islamic full-face 'veil'? Why is law and civil order so closely associated with robes, gowns, suits, wigs and uniforms? Why is law so concerned with the 'evident' and the need for justice to be 'seen' to be done? Why do we dress and obey dress codes at all? In this, the first ever study devoted to the many deep cultural connections between dress and law, the author addresses these questions and more. His responses flow from the radical thesis that 'law is dress and dress is law'. Engaging with sources from The Epic of Gilgamesh to Shakespeare, Carlyle, Dickens and Damien Hirst, Professor Watt draws a revealing history of dress and civil order and offers challenging conclusions about the nature of truth and the potential for individuals to fit within the forms of civil life
Letter from Gary Okihiro, professor, Department of Asian American Studies at Cornell University to Michi Weglyn
No full textA letter from Asian American studies professor Gary Okihiro to Michi Weglyn apologizing for a critical review he wrote of her book "Years of Infamy" in 1977.These materials are from box 73 and 74 of the Frank Chin Papers. The Frank Chin Papers contain personal and professional correspondence between Frank Chin and Michi Weglyn relating to particular projects on which either author was working as well as files related to the Day of Remembrance Tribute to Michi Weglyn
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