1,721,570 research outputs found
<i>Et in Arcadia Ego</i>: Addressing Cancer, Death and Immortality Using Science
No full textAbstract
Charlotte Jarvis has collaborated with Hans Clevers to grow her own tumor. Here, the authors discuss the project’s aims to examine mortality and create a dialogue with and about cancer.</jats:p
Das Potenzial von Organoiden realisieren : ein Interview mit Hans Clevers ; aus dem Englischen übersetzt von Rike Zietlow
No full textEs handelt sich um ein Interview mit Hans Clevers, einem Pionier auf dem Forschungsgebiet der Organoide
The Next Generation of Organoids Will Be More Complex and Even Closer to Resembling Real Organs: An Interview with Prof. Dr. Hans Clevers
No full textIn this issue, we are pleased and honored to have an interview with Professor Hans Clevers, who is the Advisory Board Member of Organoids [...
Abstract IA6: Lgr5 stem cell-based organoids in human disease
No full textAbstract
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We originally defined Lgr5 as a Wnt target gene, transcribed in colon cancer cells. Two knock-in alleles revealed exclusive expression of Lgr5 in cycling, columnar cells at the crypt base. Using lineage tracing experiments in adult mice, we found that these Lgr5+ve crypt base columnar cells (CBC) generated all epithelial lineages throughout life, implying that they represent the stem cell of the small intestine and colon. Lgr5 was subsequently found to represent an exquisitely specific and almost ‘generic’ marker for stem cells, including in hair follicles, kidney, liver, mammary gland, inner ear tongue and stomach epithelium.
Single sorted Lgr5+ve stem cells can initiate ever-expanding crypt-villus organoids, or so called ‘mini-guts’ in 3D culture. The technology is based on the observation that Lgr5 is the receptor for a potent stem cell growth factor, R-spondin. Similar 3D cultures systems have been developed for the Lgr5+ve stem cells of human stomach, liver, pancreas, prostate and kidney. Using CRISPR/Cas9 technology, genes can be efficiently modified in organoids of various origins.
Citation Format: Hans Clevers. Lgr5 stem cell-based organoids in human disease [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr IA6.</jats:p
Abstract IA04: Lgr5 stem cell-based organoids in human disease
No full textAbstract
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We originally defined Lgr5 as a Wnt target gene, transcribed in colon cancer cells. Two knock-in alleles revealed exclusive expression of Lgr5 in cycling, columnar cells at the crypt base. Using lineage tracing experiments in adult mice, we found that these Lgr5+ve crypt base columnar cells (CBC) generated all epithelial lineages throughout life, implying that they represent the stem cell of the small intestine and colon. Lgr5 was subsequently found to represent an exquisitely specific and almost ‘generic’ marker for stem cells, including in hair follicles, kidney, liver, mammary gland, inner ear tongue and stomach epithelium.
Single sorted Lgr5+ve stem cells can initiate ever-expanding crypt-villus organoids, or so called ‘mini-guts’ in 3D culture. The technology is based on the observation that Lgr5 is the receptor for a potent stem cell growth factor, R-spondin. Similar 3D cultures systems have been developed for the Lgr5+ve stem cells of human stomach, liver, pancreas, prostate and kidney. Using CRISPR/Cas9 technology, genes can be efficiently modified in organoids of various origins.
Citation Format: Hans Clevers. Lgr5 stem cell-based organoids in human disease. [abstract]. In: Proceedings of the AACR Special Conference: Patient-Derived Cancer Models: Present and Future Applications from Basic Science to the Clinic; Feb 11-14, 2016; New Orleans, LA. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(16_Suppl):Abstract nr IA04.</jats:p
Identification of response pathways of prostate cancer cell lines in Hans Clevers Media
No full textProstate cancer has been extremely difficult to grow in vitro, however recent development of a novel media has improved success rates of propagating tumour cells in the laboratory. This project aimed to define pathways regulated by this media in different prostate cancer cells in order to identify critical factors needed for prostate cancer survival. Both common and cell line-specific responses to the media were identified, demonstrating that not all prostate cancer cells respond the same way or have the same requirements. These unique responses must be considered when cells grown in this media are used to explore potential new treatment targets
Développement, cancer et cellules souches
No full textColloque organisé par Martine Roussel (St Jude, Danny Thomas Research Center, Memphis) et Hans Clevers (Hubrecht Institute, Utrecht) du 23 au 28 août 2010 Participants Yann Barrandon, Eduard Battle, Hans Clevers, Bruce A. Edgar, Riccardo Fodde, Harry Heimberg, Joerg Huelsken, Philippe Jay, Luis F. Parada, Martine Roussel, Owen Sansom, Charles J. Sheer, Rune Toftgard, Johan va Es, Maarten van Lohuizen, Fiona Watt -- Compte-rendu The theme of the meeting was inspired by the ever increasing role..
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