1,631 research outputs found
Novel function of POSH, a JNK scaffold, as an E3 ubiquitin ligase for the Hrs stability on early endosornes
No full textPOSH (plenty of SH3s) acts as a scaffold that links activated Rac1 and downstream c-Jun N-terminal kinase (JNK) signaling modules. However, it is unknown whether it's functional domain-mediated roles including the interesting RING-finger domain or its cellular function. Here, we provide evidence that subcellular localization of POSH is regulated by a particular domain of the protein and POSH was colocalized with hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) on early endosomes via interaction of Hrs with POSH's two rear SH3 domains. Moreover, the RING domain of POSH specifically regulates the stability of Hrs, but not of JNK1, via a ubiquitin-proteasomal degradation pathway. Finally, we demonstrate that JNK1 does not interact with Hrs under the conditions of POSH interacted with Hrs, but instead reduces the POSH-catalyzed ubiquitination of Hrs and their reciprocal interaction. Together, these data suggest that POSH has a distinct role as a specific E3 ubiquitin ligase for Hrs on early endosomes, and there exists a relationship between its separate activities as a scaffold and as an E3. (c) 2005 Elsevier Inc. All rights reserved.X1125sciescopu
Efficacy and safety of hypertonic saline solutions in the treatment of severe head injury.
No full textSynthesis and characterization of group 4 constrained geometry complexes containing a linked cyclopentadienyl-o-carboranyl ligand
No full textNovel linked cyclopentadienyl-o-carboranyl group 4 metal complexes, [eta (5):eta (1)-Me2Si(C5Me4)(C2B10H10]TiCl2 (2) and [eta (5):eta (1)-Me2Si(C5Me4)(C2B10H10)]Zr(NMe2)(2) (3), have been prepared via salt metathesis and amine elimination. reactions utilizing the linked o-carboranyl ligand, Me2Si(C5Me4H)(C2B10H11) (1). Their X-ray crystal structures are reported.The financial support from the
Korea Science and Engineering Foundation and the
Brain Korea 21 Project are gratefully acknowledged
Successful resuscitation of acute massire pulmonary embolism with extracorporeal membrane oxygenation and open embolectomy.
No full textDelayed diagnosis of esophageal perforation following intraoperative transesophageal echocardiography during valvular replacement--- a cae report.
No full textNeglected esophageal injury presenting with spontaneously shrunken retroesophageal pocket
No full textColor doppler ultrasonography in detecting transdiaphragmatic flow of hepatic hydrothorax: correlated with thoracoscopic findings
No full textIsospecific propylene polymerization by C-1-symmetric Me2Si(C5Me4)(2-R-Ind)MCl2 (M = Ti, Zr) complexes
No full textNovel Cl-symmetric group 4 ansa-metallocene complexes Me2Si(C5Me4)(2-R-Ind)MCl2 (R = H, M = Ti (5a), Zr (5b); R = Me, M = Ti (6a), Zr (6b)) were prepared from the reaction of dilithium salt of the corresponding ligands Me2Si(C5Me4H)(2-R-IndH) (R = H, Me) with appropriate group 4 metal halides. Crystal structures of 5a, 6a, and 6b determined by X-ray diffraction studies reveal chiral C-1-symmetric nature irrespective of the type of metal ana ligand. The complexes 5a, 5b, 6a, and 6b were tested for the polymerization of propylene in the presence of methylaluminoxane (MAO) at various temperatures. All of them exhibit good activity and afford moderate to high isotactic polypropylenes. In the case of titanocenes (5a and 6a), polymerization activity, molecular weight, and isotacticity of polypropylene sharply increase as the polymerization temperature decreases, while the increase of polymerization activity and the decrease of isotacticity and molecular weight of polypropylene were observed upon increasing the polymerization temperature in the case of zirconocenes (5b and 6b). The effect of a 2-Me substituent at the indenyl ring on propylene polymerization was apparent in terms of polymerization activity and molecular weight of polypropylene, especially for zirconocenes, but was minor for titanocenes.Financial support from the Korea
Science and Engineering Foundation (R02-2002-000-
00057-0) is gratefully acknowledged
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