8,049 research outputs found
Former basketball coaches Wally Halas and Barbara Bardy Stevens converse at Clark Athletics Hall of Fame induction ceremony, 1994
No full textPhotograph of former Clark University basketball coaches Wally Halas and Barbara Bardy Stevens conversing at the Clark Athletics Hall of Fame induction ceremony, 1994. Halas was inducted that year while Stevens would be inducted in 2006.
All photographs in this collection were digitized between 2022 and 2023.
The photographs in this collection are part of the Photographs and Media record group of Clark University’s Archives & Special Collections.https://commons.clarku.edu/basketball/1058/thumbnail.jp
Identification of molecules that inhibit activity of Escherichia coli or Staphylococcus aureus LexA protein
No full textOdziv SOS pri bakterijah se sproži ob poškodbah DNA in bakteriji omogoči, da ohrani integriteto lastnega genoma. Poglavitni regulator odziva je transkripcijski faktor LexA, ki z vezavo na promotorska področja prepreči prepis genov, katerih produkti so vključeni v popravilo DNA. Genom bakteriofaga GIL01 vključuje gen za protein gp7, ki se veže s proteinom LexA bakterije Bacillus thuringiensis. Protein gp7 zveča afiniteto LexA do specifičnih nukleotidnih zaporedij in inhibira s proteinom RecA sproženo inaktivacijo LexA. Posledično protein gp7 vpliva na aktivacijo odziva SOS, kar potencialno zavre prilagoditev bakterij na stresne razmere v okolju, tudi pridobitev odpornosti proti antibiotikom. Namen magistrskega dela je bil prepoznati molekule, ki vplivajo na delovanje represorja LexA, podobno kot protein gp7. V ta namen smo izolirali protein gp8 temperatnega bakteriofaga GIL01 bakterije B. thuringiensis in protein OrbA temperatnega bakteriofaga ICP1 bakterije Vibrio cholerae. Rezultati nakazujejo, da se izolirana rekombinantna proteina ne vežeta s proteinom LexA gostiteljske bakterije in ne vplivata na cepitev proteina LexA. V nadaljevanju smo analizirali vpliv nekaterih izbranih malih molekul, pridobljenih iz laboratorija prof. dr. Stanislava Gobca iz Fakultete za farmacijo, na aktivnost LexA. Z uporabo površinske plazmonske resonance smo dokazali, da se učinkovina 6-hidroksiflavon (U5) s šibko afiniteto veže s proteinom LexA bakterij Escherichia coli in Staphylococcus aureus ter inhibira vezavo LexA bakterije E. coli na DNA. S poskusi in vivo, v tekoči kulturi, smo dokazali, da dodatek U5 upočasni rast bakterijske kulture E. coli ΔacrB in deluje sinergistično z antibiotikom ciprofloksacin. Učinkovina U5 je šibko zavrla tudi rast seva E. coli RW542, ki ima okvarjen gen za LexA in okvarjen promotorski element gena sulA, zato predvidevamo, da U5 inhibitorno vpliva tudi na procese, ki niso del odziva SOS.SOS response in bacteria is triggered upon DNA damage. It enables bacteria to maintain the integrity of their genome. The key regulator of the SOS response is the transcription factor LexA. LexA binds to promoter regions and represses the induction of SOS genes involved in DNA damage repair. The bacteriophage GIL01 encodes the gp7 protein, which interacts with LexA from the bacterium Bacillus thuringiensis. The gp7 protein increases the affinity of LexA for target DNA sites and inhibits RecA-induced self-cleavage of LexA. Therefore, the gp7 protein affects the induction of the SOS response which presumably inhibits the adaptation of bacteria to harsh environmental conditions, such as antibiotic stress. The aim of this work was to identify molecules that have the potential to affect the activities of LexA, similar to the gp7 protein. Therefore, we purified the recombinant proteins: the gp8 protein of the temperate bacteriophage GIL01, which infects the bacterium B. thuringiensis and the recombinant protein OrbA of the bacteriophage ICP1 that infects bacterium Vibrio cholerae. Our results indicate that the OrbA and gp8 proteins do not interact with the protein LexA of the host bacterium and do not affect the self-cleavage activity of LexA. Next, we tested the effect of selected small compounds that we obtained from the laboratory of Prof. Dr. Stanislav Gobec of the Faculty of Pharmacy, on LexA activity. Using surface plasmon resonance spectroscopy, we showed that the molecule 6-hydroxyflavone (U5) has a low affinity for the LexA protein of Escherichia coli and Staphylococcus aureus and inhibits the binding of LexA from E. coli to the target DNA sites. Using in vivo experiments, we demonstrated that U5 inhibits the growth of the E. coli ΔacrB strain and acts synergistically with the antibiotic ciprofloxacin. Furthermore, the molecule U5 weakly inhibited the growth of strain E. coli RW542, which carries the frameshift mutation in the lexA gene and the deleted promoter element of the sulA gene. Therefore, we hypothesize that compound U5 also affects processes other than the SOS response
Barbara James
No full textDate:1943Barbara was born in Holdredge, Nebraska in the United States of America in 1943. In 1960 she arrived in Darwin working in a variety of occupations such as a journalist, historian, author, activist, advocate and editor. Barbara wrote 13 books including "No Man's Land" which explored the contributions of women in the Northern Territory. She also received a number of awards including 2001 NT Heritage Award, the 2000 NT Literary Essay Awards and the Chief Minister's Women's Achievement Award in 1999.JournalistHistorianAuthorActivistEditorAmerica
Barbara Ras - Sowell Conference 2017
No full textBarbara Ras, San Antonio, Poet, author of "Bite Every Sorrow" and "The Last Skin
Exclusive interview with author Barbara Kingsolver
No full textExclusive interview with author Barbara Kingsolver for her 2018 novel *Unsheltered
Dataset for publication: Post‐war architecture and urban planning as means of reinventing Opole’s past and identity
No full textThe collection includes files related to the publication: Barbara Szczepańska, Post‐War Architecture and Urban Planning as Means of Reinventing Opole’s Past and Identity, „Urban Planning”, Vol 8, No 1 (2023): Bombed Cities: Legacies of Post-War Planning on the Contemporary Urban and Social Fabric, pp. 266-278, https://doi.org/10.17645/up.v8i1.6079. The collection includes figures used in the publication:Opole_plan A plan of Opole, with areas of Ostrówek (left), Market Square (center) and Central Square (right) highlighted in red. Originally published in: "Guidebook to the city of Opole" ("Przewodnik po mieście Opolu", Opole: Księgarnia Opolska, 1948, https://polona.pl/preview/2f383a4a-5e9e-444d-9e94-366b8ac8610d). Author: Z. Streer. Licence: CC0Opole_Monument to the Opole Silesian Fighters for Freedom A photograph depicting Monument to the Opole Silesian Fighters for Freedom (Pomnik Bojownikom o Wolność Śląska Opolskiego) in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_monument of Kazimierz I Opolczyk A photograph depicting the monument of Kazimierz I Opolczyk in the Market Square in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_Market Square_eastern frontage A photograph depicting eastern frontage of the Market Square in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_Market Square_eastern frontage_before 1945 A photograph depicting eastern frontage of the Market Square in Opole before 1945. Originally published on Wikimedia Commons: https://commons.wikimedia.org/wiki/File:Market_Square_in_Opole,_eastern_frontage.jpg. Author: unknown. Licence: CC0Opole_monument of Frederick the Great A photograph depicting monument of Frederick the Great in Opole, before 1945. Originally published on Wikimedia Commons: https://commons.wikimedia.org/wiki/File:Opole_Oppeln_Denkmal_Friedrich_der_Große.jpg. Author: unknown. Licence: CC0</ul
'A date with Barbara': paracosms of the self in biographies of Barbara Newhall Follett
No full textIn 1927, 13-year-old Barbara Newhall Follett published her first book, the critically acclaimed novel, The House Without Windows and Eepersip's Life There.
Twelve years later, on December 7, 1939, 25-year-old Barbara quarrelled with her husband and left her apartment in Boston with $30 in her pocket, and a notebook. She was never seen again.
The House Without Windows is set in a paracosm (Farksolia) she invented, and ends with the metamorphosis of the titular character into a 'fairy-a wood nymph … invisible for ever to all mortals, save those few who have minds to believe, eyes to see'.
In Barbara's (auto)biography, The Unconscious Autobiography of a Child Genius (1966), written by Harold Grier McCurdy 'in collaboration with Helen Follett' (Barbara's mother), the authors wonder: 'Can we be far wrong in substituting Barbara's name for Eepersip's in the closing scenes of [House Without Windows]?
In this paper, I grapple with the formal and ethical challenges of writing about Barbara Newhall Follett, and the ways her family and others have approached the problem of writing her unresolved life story: a child raised and educated in solitude, a celebrated 'natural' child author, a young woman whose disappearance remains unsolved. The paper will explore the ways in which adults write the stories of children's lives, as nostalgia and fable, as fairytale and paracosmic narrative, and the ways in which Barbara's biographers have, consciously and unconsciously, created biographical concordances, or paracosms of the self, in seeking to make meaning of her life's story
Barbara Ehrenreich: Blood Rites: A New Evolutionary Perspective on Violence
No full textBarbara Ehrenreich, author, social critic and political essayist, discusses the emotional and social aspects of warfare and violence.
Barbara Ehrenreich is an American author and political activist who describes herself as a myth buster by trade” and has been called a veteran muckraker by The New Yorker.During the 1980s and early 1990s she was a prominent figure in the Democratic Socialists of America. She is a widely read and award-winning columnist and essayist, and author of 21 books. Ehrenreich is perhaps best known for her 2001 book Nickel and Dimed: On (Not) Getting By in America
Barbara Dicker Oration 2018 - The phenomenon of hallucinations
No full textThe 2018 Barbara Dicker Oration was presented by Professor Iris Sommer on 13 September 2018. Professor Sommer is a best-selling author and Professor of Cognitive Aspects of Neurological and Psychiatric Disorder at the Department of Neuroscience at the University Medical Center Groningen, Netherlands. Entitled The phenomenon of hallucinations, Professor Sommer offered a holistic view into the research and experiences of hallucinations. It’s actually more common than you might think but what happens in our brains when we hallucinate? And what does this mean for new treatments and interventions
Designer: from author to creative commons
No full textThe essay explores the transformations that have occurred in the role of design, from authorship to networking, sharing and opensource modes
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