1,361 research outputs found
Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains
No full textThe war between plants and their pathogens is endless, with plant resistance genes offering protection against pathogens until the pathogen evolves a way to overcome this resistance. Given how quickly new pathogen strains can arise and defeat plant defenses, it is critical to more rapidly identify and examine the specific genomic characteristics new virulent strains have gained which give them the upper hand. An indispensable tool is bioinformatics. Genome sequencing has advanced rapidly in the last decade, and labs are frequently uploading high-quality genomes of various organisms, including plant pathogenic bacteria such as Pseudomonas syringae. Pseudomonas syringae strains inject several effector proteins into host cells which often overcome host defenses. Probing online genomes provides a way to quickly and accurately predict effector repertoires of Pseudomonas, enabling the cloning of complete effector libraries of newly emerged strains. Here, we describe detailed protocols to rapidly clone bioinformatically predicted P. syringae effectors for various screening applications. © Springer Science+Business Media, LLC, part of Springer Nature 2019.11Nscopu
Obituary: Dr M.K. Bhan – A True Leader & Visionary
No full text12The author remembers Dr M.K. Bhan as a true leader, visionary, a passionate scientist, doctor and a wonderful human being, who has given love and affection to everyone around him
M.K. Asante: “It’s Bigger than Hip Hop: Art, Race and Politics”
No full textIncludes descriptive metadata provided by producer in MPEG-4 video file: "Arts and Culture - Video - M.K. Asante: 'It’s Bigger than Hip Hop: Art, Race and Politics.'" By M.K. Asante. Author and filmmaker Asante delivers the annual Walter R. Murray, Jr., Lecture on Oct. 20, 2010 at the Commons Center. He takes questions after his lecture
A Unified Shell model for Buoyancy-Driven Turbulence
No full textWe construct a unified shell model for stably stratified and convective turbulence. Shell model simulation of stably stratified flow in turbulent regime exhibit Bolgiano-Obukhbov (BO) scaling in which the kinetic energy spectrum varies as . However, simulation of convective turbulence shows Kolmogorov's spectrum. These results are consistent with the direct numerical simulations of Kumar {\em et al.} [Phys. Rev. E {\bf 90}, 023016 (2014)]. We also observe a dual scaling ( and ) for a limited range of parameters in stably stratified flow
Sweeping has no effect on renormalized turbulent viscosity
No full textWe perform renormalization group analysis (RG) of the Navier-Stokes equation in the presence of constant mean velocity field , and show that the renormalized viscosity is unaffected by , thus negating the ``sweeping effect", proposed by Kraichnan [Phys. Fluids {\bf 7}, 1723 (1964)] using random Galilean invariance. Using direct numerical simulation, we show that the correlation functions for and differ from each other, but the renormalized viscosity for the two cases are the same. Our numerical results are consistent with the RG calculations
Energy transfers in small-scale and large-scale dynamos
No full textWe study energy transfers during magnetic energy growth in small-scale and large-scale dynamos. We perform direct numerical simulations for magnetic Prandtl number Pm =20 and 0.2 in a periodic box on 1024^3 grid. Energy fluxes and shell-to-shell energy transfers indicate that in small-scale dynamo for Pm =20, the magnetic energy growth takes place due to a non-local energy transfer from large-scale velocity field to small-scale magnetic field. On the other hand, in large-scale dynamo for Pm =0.2, local energy transfers from large-scale velocity field to large-scale magnetic field takes place
Author Correction: Long-range chiral exchange interaction in synthetic antiferromagnets
No full textIn the version of this Article originally published, the sentence ‘D.-S.H. wrote the paper with K.L., J.H. and M.K.’ in the author contributions was incorrect; it should have read ‘D.-S.H. wrote the paper with K.L., J.H., M.-H.J. and M.K.’ This has been corrected in the online versions of the Article
Implicit large-eddy simulation of the stratocumulus-topped boundary layer: A grid sensitivity study
No full textA range of implicit large-eddy simulations of the stratocumulus-topped boundary layer is performed to study the influence of grid resolution on selected parameters including liquid water path and second and third moments of vertical velocity fluctuations. The simulations are based on two sets of aircraft measurements, which are also used to evaluate the results of the simulations. The specific case presented here indicates that simulations with a grid aspect ratio accounting for the anisotropic nature of the turbulence near the surface and at the top of the boundary layer lead to better agreement with measurements than simulations with an isotropic grid
Author correction: Clear and transparent nanocrystals for infrared-responsive carrier transfer
No full textThe original version of this Article omitted the fourth author Taizo Yoshinaga, who is from the 'Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Japan'. Consequently, the third sentence of the Author Contributions, 'M.S. and M.K. synthesized the ITO NCs and ITO/semiconductor oxides' was revised to 'M.S., M.K. and T.Y. synthesized the ITO NCs and ITO/semiconductor oxides'. This has been corrected in both the PDF and HTML versions of the Article
Sense of “Axial time” in A.V. Semushkin and M.K. Mamardashvili’s creativity
No full textIn the article considers the concept of “axial time”, developed by K. Jaspers. Through a prism of A.V. Semushkin and M.K. Mamardashvili’s creativity opens its essence determined as “spiritual revolution”. Defined its specific features, as consequence of this “break to transcendent”: universalism, an opportunity of the communication, birth of philosophy, world religions and sciences; history, person and morals. Degradation the modern culture, expressing in growth of conflicts and wars, communicate the author with deviation from revelations of “axial time” that threatens mankind with self-destruction
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