6 research outputs found
Maternal dietary diversity and associated factors with a focus on the food environment in the Tigray region, Northern Ethiopia
No full textBackground: Women's diet diversity is a proxy indicator of micronutrient adequacy. Low diet diversity affects the health of pregnant women and their offspring, eventually hindering productivity and economic development. Despite its significant influence on nutrition, the food environment has been considered to a lesser extent in international research and advocacy. Currently, influencing the food environment and increasing nutritional sensitivity are emerging strategies for addressing nutritional challenges. Therefore, this study aimed to assess diet diversity and associated factors, with a special focus on the food environment, among pregnant women in the Kilteawlaelo district, Tigray, northern Ethiopia. Methods: A mixed cross-sectional study design was used. The quantitative part of the study consisted of a total of 423 randomly selected pregnant women. Seven focus group discussions and seven in-depth interviews were also conducted in the qualitative study. Quantitative data were analyzed using SPSS version 21. The maternal diet diversity score was defined as adequate if it was greater than or equal to five food groups. Bivariate and multivariable binary logistic regressions were used to analyze the data. Qualitative data were transcribed and entered into the Atlas Ti version 7.5 software for coding, and inductive thematic analysis was used. Results: Only 48.2% (95% CI: 43.00, 52.70) of pregnant women had adequate diet diversity. Being a farmer (p = 0.010), having low food availability perceptions (p = 0.003), and having only one local market day (p = 0.013) were associated with low diet diversity. Pregnant women reported in their group discussions that foods were not available at home in sufficient quantities, were not affordable in the market, and they had to travel far to reach the local food markets. Conclusion: A large number of pregnant women had inadequate dietary diversity scores, which can imply micronutrient deficiencies. While occupation, perceived food availability, and number of market days in a week had significant effects on diet diversity, food desirability, convenience, and market accessibility were not associated with it. Improving the food environment by increasing the availability of food and expanding local food markets and the number of market days can lower the prevalence of inadequate diet diversity among pregnant women.</p
Tsetse bloodmeal analyses incriminate the common warthog Phacochoerus africanus as an important cryptic host of animal trypanosomes in smallholder cattle farming communities in Shimba Hills, Kenya
No full textTable S1: Data on tsetse fly bloodmeal hosts and trypanosome
infections in the different study-blocks in Shimba Hills, Kenya.Data Availability Statement: The dataset used and/or analysed during the current study are available from the corresponding author FIE on reasonable request. DNA sequences of vertebrate
species generated during the current study are available in the GenBank under accession numbers:
MZ816958-MZ816971.Trypanosomes are endemic and retard cattle health in Shimba Hills, Kenya. Wildlife in the area act as reservoirs of the parasites. However, wild animal species that harbor and expose cattle to tsetse-borne trypanosomes are not well known in Shimba Hills. Using xeno-monitoring surveillance to investigate wild animal reservoirs and sources of trypanosomes in Shimba Hills, we screened 696 trypanosome-infected and uninfected tsetse flies for vertebrate DNA using multiplegene PCR-High Resolution Melting analysis and amplicon sequencing. Results revealed that tsetse flies fed on 13 mammalian species, preferentially Phacochoerus africanus (warthogs) (17.39%, 95% CI: 14.56–20.21) and Bos taurus (cattle) (11.35%, 95% CI: 8.99–13.71). Some tsetse flies showed positive cases of bloodmeals from multiple hosts (3.45%, 95% CI: 2.09–4.81), including warthog and cattle (0.57%, 95% CI: 0.01–1.14). Importantly, tsetse flies that took bloodmeals from warthog had significant risk of infections with Trypanosoma vivax (5.79%, 95% CI: 1.57–10.00), T. congolense (7.44%, 95% CI: 2.70–12.18), and T. brucei sl (2.48%, 95% CI: 0.33–5.29). These findings implicate warthogs as important reservoirs of tsetse-borne trypanosomes affecting cattle in Shimba Hills and provide valuable epidemiological insights to underpin the parasites targeted management in Nagana vector control programs in the area.A German Academic Exchange Service (DAAD) in-region postgraduate scholarship; the BioVision Foundation Switzerland; European Union’s Integrated Biological Control Applied Research Programme—tsetse repellent component; the German Ministry for Economic Cooperation and Development (BMZ) through the Deutsche Gesellschaft für Internationale Zusammenarbeit; UK’s Department for International Development (DFID); Swedish International Development Cooperation Agency (SIDA); the Swiss Agency for Development and Cooperation (SDC); and the Kenyan Government.https://www.mdpi.com/journal/pathogensam2022Zoology and Entomolog
Dietary diversity and related factors among lactating women visiting public health facilities in Aksum town, Tigray, Northern Ethiopia
No full textBACKGROUND: Dietary diversity is a proxy indicator of maternal nutrient adequacy. However, little is documented on dietary diversity among lactating mothers. Therefore, this study assessed diet diversity and associated factors among lactating mothers visiting public health facilities in Aksum town, Tigray, Northern Ethiopia. METHODS: An institution based cross-sectional study was conducted on 346 randomly selected lactating women visiting public health facilities of Aksum town in June 2015. Dietary diversity was assessed using 24 h dietary recall method. A mean dietary diversity score (DDS) was computed of nine food groups. Food insecurity access was measured using 9 items Household Food Insecurity Access Scale (HFIAS). Data were entered and analyzed using SPSS version 20. RESULTS: More than a quarter (27.2 %) of the lactating mothers was from food insecure households. The mean DDS was 3.4 and a total of 56.4 % lactating mothers had low dietary diversity (less than mean DDS). Average monthly income, home gardening and source of drinking water were associated with low dietary diversity. Lactating mothers who had monthly income of less than 501 ETB [AOR = 2.3, 95 % CI (1.2, 4.6)] and 501 to 1500 ETB [AOR = 3.0, 95 % CI (1.4, 6.3)] were more likely to have low dietary diversity than those who had monthly income above 1500 ETB. And those who did not practice home gardening were more likely to have low dietary diversity than their counterparts [AOR = 2.1, 95 % CI (1.01, 4.2)]. Regarding source of drinking water, mothers who reported protected well as a main source were more likely to have low dietary diversity compared to those who reported tap water as main source [AOR = 4.5, 95 % CI (1.1, 18.2)]. CONCLUSIONS: Dietary diversity among lactating mothers was low. Factors like monthly income less than 501 ETB and 501 to 1500 ETB, not having home gardening, and source of drinking water from protected well were significantly associated with low dietary diversity. Attention should be paid to dietary diversity of lactating mothers to improve their nutritional status, and that of their children and their family
Factors associated with localization of tuberculosis disease among patients in a high burden country: A health facility-based comparative study in Ethiopia
No full textIntroduction: In contrast to most tuberculosis (TB) high burden countries, Ethiopia has for a long time reported a very high percentage of extra pulmonary TB (EPTB), which is also reflected in population based estimations reported by the World Health Organization (WHO). Particularly a steadily higher proportion of cervical tuberculous lymphadenitis (TBLN) has been described. Here we identify clinical and demographic factors associated with anatomic site of the TB disease.Method: A health facility based comparative study was conducted among TBLN and PTB patients who visited selected health facilities in Ethiopia during 2016 and 2017. Associated risk factors were identified through a multivariate logistic regression model using R-studio.Result: A total of 1,890 study participants, 427 TBLN and 1,463 PTB patients, were included. The mean age of TBLN patients (29 years +/- 14.4 SD) was lower than that of PTB cases (36 years +/- 15.0 SD). There were slightly more women diagnosed with TBLN (51.1%) while nearly 6 out of 10 male patients were diagnosed with PTB (58.9%). Most significantly, younger age groups (56 years), with an AOR of 9.76 (95% CI: 4.87, 19.56). The odds of cervical TBLN among women [1.69 (1.30, 2.20)] was higher than that for men. In addition, adjusted estimates suggested that, compared with PTB, renal diseases [3.41 (1.29, 9.02)] and the presence of other concomitant chronic illness [1.61 (1.23, 2.09)] had a significant association with TBLN.Conclusion: Generally, the risk of developing a particular form of TB disease is usually associated with demographic and medical history of an infected individual. Hence, the current symptom based screening, which primarily rely on chronic cough in many countries, may lead to missing significant portions of TBLN cases.We would like to forward our appreciation for those working staff of the Armauer Hansen Research Institute and all members of the ETHICOBOTS project who had a great contribution to the success of this study. Besides, we would like to extend our acknowledgment to the University of Gondar and the academic staff of the public health institute. This work was funded by the Biotechnology and Biologic Sciences Research Council, the Department for International Development, the Economic & Social Research Council, the Medical Research Council, the Natural Environment Research Council and the Defence Science & Technology Laboratory, under the Zoonoses and Emerging Livestock Systems (ZELS) program, ref: BB/L018977/1. SB was also partly funded by the Department for Environment, Food & Rural Affairs, United Kingdom, ref: TBSE3294. The members of the Ethiopia Control of Bovine Tuberculosis Strategies (ETHICOBOTS) consortium are: Abraham Aseffa, Adane Mihret,
Bamlak Tessema, Bizuneh Belachew, Eshcolewyene Fekadu, Fantanesh Melese, Gizachew Gemechu, Hawult Taye, Rea Tschopp, Shewit Haile, Sosina Ayalew, Tsegaye Hailu, all from Armauer Hansen Research Institute, Ethiopia; Rea Tschopp from Swiss Tropical and Public Health Institute, Switzerland; Adam Bekele, Chilot Yirga, Mulualem Ambaw, Tadele Mamo, Tesfaye Solomon, all from Ethiopian Institute of Agricultural Research, Ethiopia; Tilaye Teklewold from Amhara Regional Agricultural Research Institute, Ethiopia; Solomon Gebre, Getachew Gari, Mesfin Sahle, Abde Aliy, Abebe Olani, Asegedech Sirak, Gizat Almaw, Getnet Mekonnen, Mekdes Tamiru, Sintayehu Guta, all from National Animal Health Diagnostic and Investigation Centre, Ethiopia; James Wood, Andrew Conlan, Alan Clarke, all from Cambridge University, United Kingdom; Henrietta L. Moore and Catherine Hodge, both from University College London, United Kingdom; Constance Smith at University of Manchester, United Kingdom; R. Glyn Hewinson, Stefan Berg, Martin Vordermeier, Javier Nunez-Garcia, all from Animal and Plant Health Agency, United Kingdom; Gobena Ameni, Berecha Bayissa, Aboma Zewude, Adane Worku, Lemma Terfassa, Mahlet Chanyalew, Temesgen Mohammed, Yemisrach Zeleke, all from Addis ababa University, Ethiopia
Zoonotic tuberculosis in a high bovine tuberculosis burden area of Ethiopia
No full textBackgroundTuberculosis (TB) is a major cause of ill health and one of the leading causes of death worldwide, caused by species of the Mycobacterium tuberculosis complex (MTBC), with Mycobacterium tuberculosis being the dominant pathogen in humans and Mycobacterium bovis in cattle. Zoonotic transmission of TB (zTB) to humans is frequent particularly where TB prevalence is high in cattle. In this study, we explored the prevalence of zTB in central Ethiopia, an area highly affected by bovine TB (bTB) in cattle.MethodA convenient sample of 385 patients with pulmonary tuberculosis (PTB, N = 287) and tuberculous lymphadenitis (TBLN, N = 98) were included in this cross-sectional study in central Ethiopia. Sputum and fine needle aspirate (FNA) samples were obtained from patients with PTB and TBLN, respectively, and cultures were performed using BACTEC™ MGIT™ 960. All culture positive samples were subjected to quantitative PCR (qPCR) assays, targeting IS1081, RD9 and RD4 genomic regions for detection of MTBC, M. tuberculosis and M. bovis, respectively.ResultsTwo hundred and fifty-five out of 385 sampled patients were culture positive and all were isolates identified as MTBC by being positive for the IS1081 assay. Among them, 249 (97.6%) samples had also a positive RD9 result (intact RD9 locus) and were consequently classified as M. tuberculosis. The remaining six (2.4%) isolates were RD4 deficient and thereby classified as M. bovis. Five out of these six M. bovis strains originated from PTB patients whereas one was isolated from a TBLN patient. Occupational risk and the widespread consumption of raw animal products were identified as potential sources of M. bovis infection in humans, and the isolation of M. bovis from PTB patients suggests the possibility of human-to-human transmission, particularly in patients with no known contact history with animals.ConclusionThe detected proportion of culture positive cases of 2.4% being M. bovis from this region was higher zTB rate than previously reported for the general population of Ethiopia. Patients with M. bovis infection are more likely to get less efficient TB treatment because M. bovis is inherently resistant to pyrazinamide. MTBC species identification should be performed where M. bovis is common in cattle, especially in patients who have a history of recurrence or treatment failure
Spoligotype analysis of Mycobacterium bovis isolates from cattle and assessment of zoonotic TB transmission among individuals working in bovine TB-infected dairy farms in Ethiopia.
No full textBovine tuberculosis (bTB) is a disease with impact on dairy productivity, as well as having the potential for zoonotic transmission. Understanding the genetic diversity of the disease agent Mycobacterium bovis is important for identifying its routes of transmission. Here we investigated the level of genetic diversity of M. bovis isolates and assessed the zoonotic potential in risk groups of people working in bTB-infected dairy farms in central Ethiopia. M. bovis was isolated and spoligotyped from tissue lesions collected from slaughtered cattle as well as from raw milk collected from bTB positive cows in dairy farms from six urban areas of central Ethiopia. From consented dairy farm workers, knowledge and practices related to zoonotic TB transmission, together with demographic and clinical information, was collected through interviews. Sputum or Fine Needle Aspirate (FNA) samples were collected from suspected TB cases. Spoligotyping of 55 M. bovis isolates that originated either from cattle tissues with tuberculous lesion or from raw milk revealed seven spoligotype patterns where SB1176 was the most prevalent type (47.3%). Most isolates (89.1%) were of the M. bovis African 2 clonal complex. All sputum and FNA samples from 41 dairy farm workers with symptoms of TB were culture negative for any mycobacteria. Among the 41 TB suspected farm workers, 61% did not know about bTB in cattle and its zoonotic potential, and over two-third of these workers practiced raw milk consumption. Our spoligotype analysis suggests a wider transmission of a single spoligotype in the study area. The results reported here may be useful in guiding future work to identify the source and direction of bTB transmission and hence design of a control strategy. Isolation of M. bovis from milk, knowledge gap on zoonotic TB and practice of consumption of raw milk in the study population showed potential risk for zoonotic transmission
