691 research outputs found
High throughput single-molecule technology
No full textGreen Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BN/Chirlmin Joo LabSports & GamesBN/Bionanoscienc
Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L
No full textBackground: Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.Methods: This study examined the effect of ALBE on the release of ��-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-��B using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.Results: We observed significant inhibition of ��-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 ��g/mL) suppressed not only the transcriptional activation of NF-��B, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.Conclusions: These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-��B activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis. �� 2011 Sohn et al; licensee BioMed Central Ltd
Leaders of the field: What does the future hold for single molecule technology?
No full textIn recent years, single molecule technology has experienced a rapid growth, with exciting developments in fundamental research and real-world applications. Detecting and studying biological phenomena on thesingle molecule level requires a unique synergy between researchers working on instrumentation, physics, and the life sciences. In the iScience special issue ‘‘Single Molecule Technology – From Biotechnology toBiomedical Applications’’, guest edited by Amit Meller and Chirlmin Joo (Figure 1), we are highlighting a variety of research on nanopore technology, single molecule fluorescence, and a selection of other ultra-sensitive detection methods. More content in the special issue can be found here: https://www.sciencedirect.com/journal/iscience/special-issue/10PGSBV55N0. The guest editors in this backstory share their thoughts on what is currently exciting in the field, and the advances they think will make an impact in the near future.BN/Chirlmin Joo La
A study of two wireless telecommunications companies' globalization strategies : an analysis of Vodafone's and NTT DoCoMo's foreign investments
No full textThesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management, 2003 [first author]; and, (S.M.)--Massachusetts Institute of Technology, Sloan School of Management, 2003 [second author].Includes bibliographical references (leaves 117-119).by Hyungchul Joo and Peter D. Honkanen.M.B.A.S.M
Paving the way to single-molecule protein sequencing
No full textProteins are major building blocks of life. The protein content of a cell and an organism provides key information for the understanding of biological processes and disease. Despite the importance of protein analysis, only a handful of techniques are available to determine protein sequences, and these methods face limitations, for example, requiring a sizable amount of sample. Single-molecule techniques would revolutionize proteomics research, providing ultimate sensitivity for the detection of low-abundance proteins and the realization of single-cell proteomics. In recent years, novel single-molecule protein sequencing schemes that use fluorescence, tunnelling currents and nanopores have been proposed. Here, we present a review of these approaches, together with the first experimental efforts towards their realization. We discuss their advantages and drawbacks, and present our perspective on the development of single-molecule protein sequencing techniques.Accepted Author ManuscriptBN/Chirlmin Joo LabBN/Cees Dekker La
Unraveling proteins at the single molecule level using nanopores
No full textThe function and phenotype of a cell is determined by a complex network of interactions between DNA, RNA, proteins and metabolites. Therefore, a comprehensive approach that integrates genomics, transcriptomics, proteomics, and metabolomics is necessary to achieve full understanding of biological processes and disease. Recent technological developments have mostly focused on the study of genomes. DNA sequencing has become fast, cheap, and ubiquitous. The study of other -omes, especially the proteome, remains expensive and time-consuming...BN/Chirlmin Joo La
Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging
No full textSingle-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and transient binding of fluorescently labeled DNA probes. In its early stages the implementation of DNA-PAINT was burdened by low-throughput, excessive acquisition time, and difficult integration with live-cell imaging. However, recent advances are addressing these challenges and expanding the range of applications of DNA-PAINT. We review the current state of the art of DNA-PAINT in light of these advances and contemplate what further developments remain indispensable to realize live-cell imaging.Accepted Author ManuscriptBN/Chirlmin Joo La
Strategic plan for the built environment
No full textprepared by: the Oregon Department of Environmental Quality, Materials Management Program ; authors: Amanda Ingmire and Sun Joo Kim.Title from PDF cover (viewed on February 25, 2022).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 46-48).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Small RNA-directed DNA elimination: the molecular mechanism and its potential for genome editing
No full textTransposable elements have both detrimental and beneficial effects on their host genome. Tetrahymena is a unicellular eukaryote that deals with transposable elements in a unique way. It has a separate somatic and germline genome in two nuclei in a single cell. During sexual reproduction, a small RNA directed system compares the germline and somatic genome to identify transposable elements and related sequences. These are subsequently marked by heterochromatin and excised. In this Review, current knowledge of this system and the gaps therein are discussed. Additionally, the possibility to exploit the Tetrahymena machinery for genome editing and its advantages over the widely used CRISPR-Cas9 system will be explored. While the bacterial derived CRISPR-Cas9 has difficulty to access eukaryotic chromatin, Tetrahymena proteins are adept at acting in a chromatin context. Furthermore, Tetrahymena based gene therapy in humans might be a safer alternative to Cas9 because the latter can trigger an immune response.BN/Chirlmin Joo La
Exploring molecular biology in sequence space: The road to next-generation single-molecule biophysics
No full textNext-generation sequencing techniques have led to a new quantitative dimension in the biological sciences. In particular, integrating sequencing techniques with biophysical tools allows sequence-dependent mechanistic studies. Using the millions of DNA clusters that are generated during sequencing to perform high-throughput binding affinity and kinetics measurements enabled the construction of energy landscapes in sequence space, uncovering relationships between sequence, structure, and function. Here, we review the approaches to perform ensemble fluorescence experiments on next-generation sequencing chips for variations of DNA, RNA, and protein sequences. As the next step, we anticipate that these fluorescence experiments will be pushed to the single-molecule level, which can directly uncover kinetics and molecular heterogeneity in an unprecedented high-throughput fashion. Molecular biophysics in sequence space, both at the ensemble and single-molecule level, leads to new mechanistic insights. The wide spectrum of applications in biology and medicine ranges from the fundamental understanding of evolutionary pathways to the development of new therapeutics.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BN/BionanoscienceBN/Chirlmin Joo La
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