133 research outputs found
Corrigendum to “The 2016 update of the International Study Group (ISGPF) definition and grading of postoperative pancreatic fistula: eleven years after.” Surgery 2017. Mar; 161 (3):584–591. Epub Dec 28, 2016 (Surgery (2017) 161(3) (584–591), (S0039606016307577), (10.1016/j.surg.2016.11.014))
No full textThe authors regret that the name of author Charles R. Vollmer MD is incorrect in the final published version. The correct name Charles Vollmer. The authors would like to apologise for any inconvenience caused. Below is the correct order of authors: Claudio Bassi, MDa, Giovanni Marchegiani, MDa, Christos Dervenis, MD,b, Micheal Sarr, MDc, Mohammad Abu Hilal, MDd, Mustapha Adham, MDe, Peter Allen, MDf, Roland Andersson, MDg, Horacio J. Asbun, MDh, Marc G. Besselink, MDi, Kevin Conlon, MDj, Marco Del Chiaro, MDk, Massimo Falconi, MDl, Laureano Fernandez-Cruz, MDm, Carlos Fernandez-del Castillo, MDn, Abe Fingerhut, MDo, Helmut Friess, MDp, Dirk J Gouma, MDi, Thilo Hackert, MDq, Jakob Izbicki, MDr, Keith D. Lillemoe, MDn, John P. Neoptolemos, MDs, Attila Olah, MDt, Richard Schulick, MDu, Shailesh V. Shrikhande, MDv, Tadahiro Takada, MDw, Kyoichi Takaori, MDx, William Traverso, MDy, Charles Vollmer, MDz, Christopher L. Wolfgang, MDaa, Charles J. Yeo, MDbb, Roberto Salvia, MDa, Marcus Buchler, MDq, from the International Study Group on Pancreatic Surgery (ISGPS
Rare BRCA2 K3326X increases susceptibility to sporadic pancreatic ductal adenocarcinoma: a PANDoRA study.
No full textAbstract
Background: The incredibly poor outlook of pancreatic cancer patients underscores an urgent need for early diagnostic markers. Pancreatic cancer ranks third most-frequent among BRCA1/2-deficient cancers with germline mutations detected in <10% of sporadic cases. Germline variants in breast cancer tumor suppressor BRCA2 have been reported to increase predisposition to several cancers including pancreatic tumors. Rare K3326X (rs11571833, c.9976A>T) which introduces a premature stop codon thus truncating the protein, has previously been implicated in familial PDAC, but not in sporadic cases. A frameshift pathogenic mutation c.6503delTT (rs11571658, p.Leu2092Profs) reported to occur in tandem with K3326X in breast and ovarian cancer
families, has also been speculated to influence risk associations due to linkage disequilibrium between both variants.
Method and results: K3326X was genotyped in 2,969 sporadic cases and 4,700 controls using Taqman chemistry and fidelity of genotypes assessed based on concordance of internal replicates and negative controls. K3326X was observed in 1.2% of cases and 0.8% of controls. Odds ratios (ORs) and associated 95% confidence intervals (CIs) were estimated by multivariate unconditional logistic regression with adjustment for age, sex and region of origin. We also performed a stratified analysis based on age at diagnosis to estimate the risk association between K3326X and early-onset pancreatic cancer. To rule out the likely shared effect of the c.6503delTT mutation on sporadic PDAC risk, we also sequenced DNA from carriers of K3326X in this study. We found K3326X to be associated with increased risk of developing sporadic PDAC ((OR = 1.71, 95% CI = 1.18 - 2.49), P = 0.005). This risk was considerably higher among cases aged 50 years and younger (OR = 2.13, 95% CI = 1.10 - 4.11, P = 0.03). Furthermore, carriers of K3326X did not bear the c.6503delTT mutation thus confirming that the observed risk effect was not influenced by the latter.
Conclusion: These robust associations implicate K3326X in the etiology of sporadic and early-onset PDAC, and therefore warrant replication as well as functional studies to elucidate the role of K3326X in DNA repair mechanisms.
Citation Format: Ofure M. Obazee, Gabriele Capurso, Angelo Andriulli, Pavel Soucek, Ewa Małecka- Panas, Juozas Kupcinskas, Rudolf Kaaks, Maria Gazouli, Thilo Hackert, Aldo Scarpa, Giulia M. Cavestro, Claudio Pasquali, Hermann Brenner, Daniele Campa, Raffaele Pezzilli, Andrea Mambrini, Beatrice Mohelnikova- Duchonova, Ugo Boggi, Jakob Izbicki, Pavel Vodicka, Elzbieta Iskierka-Jazdzewska, Federico Canzian. Rare BRCA2 K3326X increases susceptibility to sporadic pancreatic ductal adenocarcinoma: a PANDoRA study [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3411. doi:10.1158/1538-7445.AM2017-3411</jats:p
sj-pdf-1-ueg-10.1177_2050640620934911 - Supplemental material for European Guideline on IgG4-related digestive disease – UEG and SGF evidence-based recommendations
No full textSupplemental material, sj-pdf-1-ueg-10.1177_2050640620934911 for European Guideline on IgG4-related digestive disease – UEG and SGF evidence-based recommendations by J-Matthias Löhr, Ulrich Beuers, Miroslav Vujasinovic, Domenico Alvaro, Jens Brøndum Frøkjær, Frank Buttgereit, Gabriele Capurso, Emma L Culver, Enrique de-Madaria, Emanuel Della-Torre, Sönke Detlefsen, Enrique Dominguez-Muñoz, Piotr Czubkowski, Nils Ewald, Luca Frulloni, Natalya Gubergrits, Deniz Guney Duman, Thilo Hackert, Julio Iglesias-Garcia, Nikolaos Kartalis, Andrea Laghi, Frank Lammert, Fredrik Lindgren, Alexey Okhlobystin, Grzegorz Oracz, Andrea Parniczky, Raffaella Maria Pozzi Mucelli, Vinciane Rebours, Jonas Rosendahl, Nicolas Schleinitz, Alexander Schneider, Eric FH van Bommel, Caroline Sophie Verbeke, Marie Pierre Vullierme, Heiko Witt and the UEG guideline working group in United European Gastroenterology Journa
Pancreatoduodenectomy with colon resection for pancreatic cancer: a systematic review
No full textBackground: Radical resection of advanced pancreatic cancer may occasionally require a simultaneous colon resection. The risks and benefits of this combined procedure are largely unknown. This systematic review aimed to assess short and long term outcome after pancreatoduodenectomy with colon resection (PD-colon) for pancreatic ductal adenocarcinoma (PDAC). Methods: A systematic literature search was performed in PubMed, Embase, and the Cochrane Library for studies published between 1994 and 2017 concerning PD-colon for PDAC. Results: After screening 2038 articles, 5 articles with a total of 181 patients undergoing PD-colon were eligible for inclusion. Included studies showed a relatively low risk of bias. The pooled complication rate was 73% (95% CI 61–84) including a pooled colonic anastomotic leak rate of 5.5%. Pooled mortality was 10% (95% CI 6–15). Pooled mean survival (data from 86 patients) was 18 months (95% CI 13–23) with pooled 3- and 5-year survival of 31% (95% CI 20–72) and 19% (95% CI 6–38). Conclusion: Based on the available data, PD-colon for PDAC seems to be associated with an increased morbidity and mortality but with survival comparable with standard PD in selected patients. Future large series are needed to allow for better patient selection for PD-colon
International consensus on definition and criteria of borderline resectable pancreatic ductal adenocarcinoma 2017
No full textThis statement was developed to promote international consensus on the definition of borderline resectable pancreatic ductal adenocarcinoma (BR-PDAC) which was adopted by the National Comprehensive Cancer Network (NCCN) in 2006, but which has changed yearly and become more complicated. Based on a symposium held during the 20th meeting of the International Association of Pancreatology (IAP) in Sendai, Japan, in 2016, the presenters sought consensus on issues related to BR-PDAC. We defined patients with BR-PDAC according to the three distinct dimensions: anatomical (A), biological (B), and conditional (C). Anatomic factors include tumor contact with the superior mesenteric artery and/or celiac artery of less than 180 degrees without showing stenosis or deformity, tumor contact with the common hepatic artery without showing tumor contact with the proper hepatic artery and/or celiac artery, and tumor contact with the superior mesenteric vein and/or portal vein including bilateral narrowing or occlusion without extending beyond the inferior border of the duodenum. Biological factors include potentially resectable disease based on anatomic criteria but with clinical findings suspicious for (but unproven) distant metastases or regional lymph nodes metastases diagnosed by biopsy or positron emission tomography-computed tomography. This also includes a serum carbohydrate antigen (CA) 19-9 level more than 500 units/ml. Conditional factors include the patients with potentially resectable disease based on anatomic and biologic criteria and with Eastern Cooperative Oncology Group (ECOG) performance status of 2 or more. The definition of BR-PDAC requires one or more positive dimensions (e.g. A, B, C, AB, AC, BC or ABC). The present definition acknowledges that resectability is not just about the anatomic relationship between the tumor and vessels, but that biological and conditional dimensions are also important. The aim in presenting this consensus definition is also to highlight issues which remain controversial and require further research. (C) 2017 IAP and EPC. Published by Elsevier B.V
Prevalence of relevant early complications during the first 24 h on a normal ward in patients following PACU care after medium and major surgery: a monocentric retrospective observational study
No full text<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Purpose</jats:title>
<jats:p>Even today, it remains a challenge for healthcare professionals to decide whether a clinically stable patient who is recovering from uncomplicated medium or major surgery would benefit from a postoperative intensive care unit (ICU) admission, or whether they would be at least as adequately cared for by a few hours of monitoring in the post-operative care unit (PACU).</jats:p>
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<jats:title>Methods</jats:title>
<jats:p>In this monocentric retrospective observational study, all adult patients who (I) underwent medium or major surgery between 1 January 1 2014 and 31 December 2018 at the Heidelberg University Surgical Center, and (II) were monitored for 1–12 h in the PACU, and then (III) transferred to a normal ward (NW) immediately thereafter were included. At the end of the PACU stay, each patient was cleared by both a surgeon and an anesthesiologist to be transferred to a NW. The first objective of this study was to determine the prevalence of relevant early complications (RECs) within the first 24 h on a normal ward. The secondary objective was to determine the prevalence of RECs in the subgroup of included patients who underwent partial pancreaticoduodenectomy.</jats:p>
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<jats:title>Results</jats:title>
<jats:p>A total of 10,273 patients were included in this study. The prevalence of RECs was 0.50% (confidence interval [CI] 0.40–0.60%), with the median length of stay in the PACU before the patient’s first transfer to a NW being 285 min (interquartile range 210–360 min). In the subgroup of patients who underwent partial pancreaticoduodenectomy (<jats:italic>n</jats:italic> = 740), REC prevalence was 1.1% (CI = 0.55–2.12%).</jats:p>
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<jats:title>Conclusion</jats:title>
<jats:p>Based on a medical case-by-case assessment, it is possible to select patients who after a PACU stay of only up to 12 h have a low risk of emergency readmission to an ICU within the 24 h following the transfer to the NW. Continued research will be needed to further improve transfer decisions in such low-risk subgroups.</jats:p>
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Special Collections 940.421 K96m.
No full textForeword by the author: The considerations by which I was induced to attempt a history of the Marne campaign, from the mobilization of the army to the retreat after the Battle of the Marne, are set forth in the Introduction.
The task at the present time is difficult. Omissions and mistakes are unavoidable in the description of a battle waged by millions of troops along a line extending from the upper Moselle through Verdun to Paris.
Many of those who took part in the war have kindly assisted me by contributions, especially Generals von Marwitz, Sixt von Armin, von Bergmann, von Stocken, Baron von Hammerstein-Gesmold, Sydow, Grautoff, Colonels Auer von Herrenkirchen, Lindenborn and von Caprivi, Lt. Col. Wetzell, Majors von Schutz, Bührmann, Köppen, Thilo, von Platen and von Voss, as also Captain Konig.
I would be grateful to any readers who send in additional material or suggest corrections.
Berlin, Steglitz, November 1920. von Kuh
Correction to: Prevalence of relevant early complications during the first 24 h on a normal ward in patients following PACU care after medium and major surgery: a monocentric retrospective observational study
No full textThe developmental cell biology of Trypanosoma brucei
No full textTrypanosoma brucei provides an excellent system for studies of many aspects of cell biology, including cell structure and morphology, organelle positioning, cell division and protein trafficking. However, the trypanosome has a complex life cycle in which it must adapt either to the mammalian bloodstream or to different compartments within the tsetse fly. These differentiation events require stage-specific changes to basic cell biological processes and reflect responses to environmental stimuli and programmed differentiation events that must occur within a single cell. The organization of cell structure is fundamental to the trypanosome throughout its life cycle. Modulations of the overall cell morphology and positioning of the specialized mitochondrial genome, flagellum and associated basal body provide the classical descriptions of the different life cycle stages of the parasite. The dependency relationships that govern these morphological changes are now beginning to be understood and their molecular basis identified. The overall picture emerging is of a highly organized cell in which the rules established for cell division and morphogenesis in organisms such as yeast and mammalian cells do not necessarily apply. Therefore, understanding the developmental cell biology of the African trypanosome is providing insight into both fundamentally conserved and fundamentally different aspects of the organization of the eukaryotic cell
Primary cilia (PC) in healthy pancreas and chronic pancreatitis (CP).
No full text(A) Intralobular duct (*) with epithelial PC. Towards larger ducts (#), only basal bodies are stained, the axonem is not developed. (B) PC-rich acinar region around an intralobular duct (*). (C) No significant expression of PC in the interlobular duct (#) while epithelial cells in smaller intralobular ducts (*) show PC. (D) Little development of PC in pancreatitis ducts. In CP, an increase of stromal PC length and number is observed in comparison to healthy pancreatic stroma around ducts (see also (G) and (H)). (E) In fibrotic areas of CP tissue, length and number of stromal PC is further increased (see also (G) and (H). (F) Tubular complexes also contain many PC. (G) Fraction of stromal PC carrying cells is higher in CP tissue than in normal pancreatic tissue (donor) in all areas assessed (Kruskal-Wallis test: p ppp (H) Stromal PC are significantly longer in the stroma of CP compared to normal pancreas (Kruskal-Wallis test p p p p < 0.05 vs. donor). Acetylated α-tubuline: red. γ-tubuline: green. DAPI: blue. Mean ± SEM.</p
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