1,219 research outputs found
The Program for in Vitro Fertilization and Embryo Transfer at National Taiwan University Hospital
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Efficacy of a Two-Marker Test Followed by Ultrasound for Antenatal Screening of Trisomy 18
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Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno-associated virus in zebrafish
No full textRelationship between Fetal Nuchal Translucency and Crown-Rump Length in an Asian Population
No full textObjectives To investigate the relationship between the nuchal translucency (NT) thickness and crown-rump length ( CRL) in normal Asian fetuses during the first trimester. Design A prospective observational study was conducted. Ultrasound measurement of NT and CRL was offered in 879 consecutive Taiwanese fetuses between 9 weeks and 14 weeks of gestation. Regression analysis was used to analyze the relationship between the NT thickness and CRL. Fetal sex was also considered in the analysis. The distribution of multiple of median (MoM) values of the NT measurements with CRL in 10-mm intervals and the 95th centile of MoM were also calculated. Results The present study shows that NT measurements increase with increasing CRL. A fixed cut-off point through the first trimester is not appropriate. The NT thickness has no relationship with fetal sex. Expressing the NT thickness by MoM values provides a simple method for clinical practice. Conclusions The present study offers normative data of the fetal NT thickness in an Asian population, which may improve the performance of NT measurement during the first-trimester as a screening tool for chromosomal aberrations or other congenital abnormalities in the first trimester
Clinicopathologic Correlation of up-Regulated Genes Identified Using Cdna Microarray and Real-Time Reverse Transcription-Pcr in Human Colorectal Cancer
No full textPurpose: We hypothesize that changes in the transcription of up-regulated genes are biologically meaningful and may be linked to variations in tumor behavior and clinical features . This study aimed to find individual up-regulated genes responsible for clinicopathologic variations in human colorectal cancer. Experimental Design: Genes up-regulated concurrently in four microarray experiments were taken as candidate genes; 20 candidate genes were verified using real -time reverse transcription-PCR in these four experiments, along with 27 new samples. The presence or absence of up - regulation of these genes was correlated with 10 clinicopathologic variables from 31 patients. The mRNA transcript levels of these 20 candidate genes in the 31 paired samples were also correlated with each other to disclose any expression relationship. Results: Forty percent (8/ 20) of the candidate genes were verified by real- time reverse transcription-PCR to have a tumor/normal expression ratio > 2. Up- regulation of THY1 and PHLAD1 was associated with the presence of anemia in colon cancer patients (P = 0.036 and 0.009, respectively). Up- regulation of HNRPA1 was more significant in cancer growing in the right- sided colon than the left side (P = 0.027). Up- regulated GPX2 was related to a higher degree of tumor differentiation (P = 0.019). c-MYC was significantly over- expressed in specimens from male compared with female colon cancer patients (P = 0.012). GRO1 was significantly up- regulated in patients younger than 65 years old (P = 0.010) and was found to be frequently over-expressed when cancers were less invasive. In addition, we found that normalized transcript levels of HNRPA1 were tightly associated with that of c-MYC (r = 0.948). Conclusions: Validation of microarray data using another independent laboratory approach is mandatory and statistical correlation between gene expression status and the patient's clinical features may reveal individual genes relevant to tumor behavior and clinicopathologic variations in human colorectal cancer
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