1,720,971 research outputs found
Antigenic heterogeneity of human mammary carcinoma cells defined by monoclonal antibodies
No full textDifferential binding to human mammary and non-mammary tumors of monoclonal antibodies reactive with carcinoembryonic antigen
No full textEvidence of enhnancement of the ras oncogene protein product (p21) in a spectrum of human tumors
No full textINT. J. CANCE
Absolute values of ras p21 defined by direct binding liquid competition radioimmunoassays.
No full textSeveral distinct and high-conserved genes comprise the ras gene family of rodents and humans, i.e., rodent Harvey and Kirsten, and human Harvey, Kirsten and neuroblastoma. Transformation, either by a point-mutation resulting in a change in one amino acid of the 21 kDa ras gene product (p21), or by increased expression of ras p21, has been demonstrated to be mediated by members of this gene family. We report here the development of direct binding liquid competition radioimmunoassays for the detection and quantitation of the ras oncogene and proto-oncogene products. Using these radioimmunoassays and ras p21 purified from Escherichia coli containing the full-length T24 mutant human Harvey ras gene protein product as a standard, we have defined the actual amount of ras p21 per micrograms of total cellular protein, or per cell, in various ras transformed and 'normal' mammalian cell lines. One of the radioimmunoassays developed is group-specific, since the antigenic determinant recognized is shared by both the point-mutated and proto-forms of Harvey, Kirsten and neuroblastoma members of the ras gene family, while the second may be termed type-selective, since it recognizes an antigenic determinant localized primarily on the Harvey ras p21. Both radioimmunoassays are interspecies, since they detect a ras p21 antigenic determinant common to cells of human and rodent origin. These studies thus describe the first means for defining absolute values of any oncogene or proto-oncogene protein product. The assays described, when used in combination with specific c-DNA probes to define specific ras proto-oncogenes or point-mutated oncogenes being expressed, will now permit truly quantitative analyses of ras p21 expression in experimental cell culture systems, animal models and human biopsy material
Definition of antigenic heterogeneity and modulation among human mammary carcinoma cell populations using monoclonal antibodies to tumor associated antigens
No full textReactivity of a monoclonal antibody (B.72.3) with fixed sections of human mammary carcinomas.
No full textPotential diagnostic and prognostic applications of monoclonal antibodies to human mammary carcinomas
No full textA monoclonal antibody (B72.3) defines pattern of distribution of a novel tumor associated antigen in human mammary carcinoma cell population
No full textMonoclonal Antibodies define differential ras gene expression in malignant and benign colonic disease
No full textGeneration and characterization of monoclonal antibodies reactive with human primary and metastatic mammary tumor cells.
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