6,683 research outputs found
Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery
No full textCopyright @ 2014 Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells
Experimental evidence for a second coordination sphere water molecule in the hydration structure of YbDTPA - Insights for a re-assessment of the relaxivity data of GdDTPA
No full textThe low temperature (-100 °C) X-ray structure of the complex K2[Yb(DTPA)(H2O)] has been determined. The metal ion is at the center of a tricapped trigonal prism and is nine-coordinate, binding to the three nitrogens and five oxygens of the ligand and one water molecule. From the structure obtained, three well-defined hydration shells can be observed consisting of: i) one coordinated water molecule; ii) several water molecules in the outer coordination sphere of the Yb(III) ion and iii) one water molecule surprisingly close to the metal center and hydrogen-bonded to proximate carboxylate groups. A variable-field and temperature NMRD study of the corresponding Gd complex has been performed and the data interpreted by taking into account the presence of second-sphere water molecule(s). The results of the analysis are in excellent agreement with the X-ray structural data
MU-HYDRIDE GEOMETRY AND DYNAMICS IN THE PROTIC ACID ADDUCTS OF TRIOSMIUM IMIDOYL CLUSTERS
No full textPHOSPHINE ADDITION AND SUBSTITUTION-REACTIONS ON UNUSUALLY REACTIVE TRIOSMIUM CLUSTERS - (μ-H)(μ3-η2-C=NCH2CH2CH2)OS3(CO)9 AND (μ-H)(μ3-η2-CH3CH2C=NCH2CH2CH3)OS3(CO)9
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Physical dance performance: An investigation into the development of a performance technique based on the integration of certain Korean dance technique and contemporary Western styles of dance and physical theatre
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University, 04/10/2001.This development of a performer practice that integrates elements of traditional Korean dance technique and Western forms of physical theatre and contemporary dance is based on an approach to internal understanding and external execution. Central to the work is the concept of body energy, or Ki. This ancient Eastern term is translated into a contemporary practice that enables a performer to engage mental and physical training. Breath and the use of breath in performing are the principal means of achieving this level of engagement
Les marqueurs de prolifération en oncologie vétérinaire : applications à l'étude pronostique du mastocytome cutané canin
No full textLe mastocytome cutané représente un type tumoral dominant de la cancérologie du chien mais demeure l'une des tumeurs canines au comportement clinique les plus imprévisibles. L'auteur évalue dans une étude rétrospective portant sur 120 chiens porteurs de mastocytome cutané, la valeur pronostique de la détection immunohistochimique des marqueurs de prolifération PCNA et Ki-67. Il montre que la détermination de l'expression de l'antigène Ki-67 permet de prédire avec une grande fiabilité l'évolution clinique post-chirurgicale de ce type tumoral
Tatari, tautoko, tauawhi - Hei awhina tamariki ki te panui pukapuka: Some preliminary findings
No full textThe Tatari, Tautoko, Tauawhi reading tutoring procedures have been adapted from the procedures known as Pause, Prompt, Praise, first developed in Mangere in 1977. The first author offered the procedures as a koha at a Special Education Service hui at Poho o Rawiri in 1991. The second author took up the koha and obtained the support of kaumatua and kuia at Hairini marae Tauranga Moana, and the support of senior Maori staff of the Special Education Service National Office to produce a Maori language video and training booklet. This began an important bicultural journey through the processes of producing instructional materials and trailing and evaluating them in ways that are biculturally appropriate. This paper reports on that journey and presents some preliminary data on the implementation of Tatari, Tautoko, Tauawhi by seven tuakana - teina pairs in a bi-lingual classroom
SYNTHESIS, STRUCTURE, AND LIGAND DYNAMICS OF TRIOSMIUM IMIDOYL CLUSTERS AND THEIR ISOCYANIDE DERIVATIVES
No full textCARBON NITROGEN CLEAVAGE AND CARBON CARBON COUPLING PROCESSES IN THE REACTIONS OF RU3(CO)12 WITH TERTIARY-AMINES
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