20 research outputs found
Hybrid Polymeric Scaffolds Prepared by Micro and Macro Approaches
No full textTurkoglu Sasmazel, Hilal/0000-0002-0254-4541; Ozkan, Ozan/0000-0002-9050-1583Polymeric scaffolds with complex porous structures were fabricated with two different polymers by combining three fabrication methods in three steps, in which, nonwoven poly(e-caprolactone) microfibers were obtained with electrospinning and immersed in solvent cast chitosan solution poured in Petri dish to fabricate hybrid polymers, and finally the combined structure was freeze-dried with two different predrying techniques to obtain macropores in the structure. The resulting hybrid polymeric mats were found to have both microfibers and macroporosity due to the electrospinning as well as freeze-drying processes, which resemble the natural extracellular matrix. The optimized scaffolds that predried in the incubator at 40 degrees C for 5 h and then freeze-dried for 24 h exhibited contact angle value of 68.93 +/- 2.18 degrees with 3.252 +/- 0.783 MPa Young's modulus and 0.260 +/- 0.002 MPa yield strength as well as 1.35-fold cell yield in MRC5 fibroblast cell culture, compared to the commercial tissue culture polystyrene. [GRAPHICS]
Novel Hybrid Scaffolds for the Cultivation of Osteoblast Cells
No full textTurkoglu Sasmazel, Hilal/0000-0002-0254-4541In this study, natural biodegradable polysaccharide, chitosan, and synthetic biodegradable polymer, poly(epsilon-caprolactone) (PCL) were used to prepare 3D, hybrid polymeric tissue scaffolds (PCL/chitosan blend and PCL/chitosan/PCL layer by layer scaffolds) by using the electrospinning technique. The hybrid scaffolds were developed through HA addition to accelerate osteoblast cell growth. Characteristic examinations of the scaffolds were performed by micrometer, SEM, contact angle measurement system, ATR-FTIR, tensile machine and swelling experiments. The thickness of all electrospun scaffolds was determined in the range of 0.010 +/- 0.001-0.012 +/- 0.002 mm. In order to optimize electrospinning processes, suitable bead-free and uniform scaffolds were selected by using SEM images. Blending of PCL with chitosan resulted in better hydrophilicity for the PCL/chitosan scaffolds. The characteristic peaks of PCL and chitosan in the blend and layer by layer nanofibers were observed. The PCL/chitosan/PCL layer by layer structure had higher elastic modulus and tensile strength values than both individual PCL and chitosan structures. The layer by layer scaffolds exhibited the PBS absorption values of 184.2; 197.2% which were higher than those of PCL scaffolds but lower than those of PCL/chitosan blend scaffolds. SaOs-2 osteosarcoma cell culture studies showed that the highest ALP activities belonged to novel PCL/chitosan/PCL layer by layer scaffolds meaning better cell differentiation on the surfaces. (C) 2011 Elsevier B.V. All rights reserved.Turkish Academy of Science (TUBA) L'Oreal; L'OrealThe author is greatly thankful to Turkish Academy of Science (TUBA) & L'Oreal for honoring this study with the award "Young Women in Science" in Materials Science in 2009. Her special thanks also go to L'Oreal for the precious financial support. The author also appreciates the invaluable contribution of AWAC (Academic Writing Advisory Center) to this study in linguistic terms
Functionalization of Nonwoven Pet Fabrics by Water/O<sub>2< Plasma for Biomolecule Mediated Cell Cultivation
No full textTurkoglu Sasmazel, Hilal/0000-0002-0254-4541The main target of this study was to obtain COOH functionalities on the surface of 3D, nonwoven polyethylene terephthalate fabrics (NWPFs) by using low pressure water/O-2 plasma assisted treatment. The plasma treatments were performed in a cylindrical, capacitively coupled RF-plasma-reactor and then following steps were performed: in situ (oxalyl chloride vapors) gas/solid reaction to convert -OH functionalities into COCl groups; and hydrolysis under open laboratory conditions using air moisture for final-COOH functionalities. COOH and OH functionalities on the surfaces were detected quantitatively by fluorescent labeling techniques. The COOH-functionalized samples were biologically activated with insulin or heparin molecules by using spacer polyoxyethylene bis-amine (PEO). Successful immobilization was checked qualitatively using electron spectroscopy for chemical analysis (ESCA). The average amount of immobilized insulin and heparin onto NWPF surfaces were determined as 146.09 and 4.81 nmol.cm(-2), respectively. Our results showed that water/O-2 plasma assisted treatment worked very well for functionalization and biofunctionalization of 3D NWPF disks comparing with wet-chemistry methods. Cell culture experiments indicated that functionalization of NWPF disks and/or nanotopographies on the disk surfaces were effective on adhesion and proliferation of L929 mouse fibroblasts
Development of Electrospun We43 Magnesium Alloy-Like Compound
No full textOzkan, Ozan/0000-0002-9050-1583; Turkoglu Sasmazel, Hilal/0000-0002-0254-4541Metallic structures are conventionally fabricated with high temperature/deformation processes resulting the smallest possible microscopic structures in the order of several hundreds of micrometer. Therefore, to obtain structures with fibers smaller than 100 Am, those are unsuitable. In this study, electrospinning, a fiber fabrication technique commonly used for polymers, was adopted to fabricate a WE43 magnesium alloy-like fibrous structure. The aim is to adopt metallic WE43 alloy to regenerative medicine using tissue engineering approach by mimicking its composition inside of a fibrous structure. The solution required for electrospinning was obtained with water soluble nitrates of elements in WE43 alloy, and PVP or PVA were added to obtain a spinnable viscosity which was pyrolised away during heat treatment. Electrospinning parameters were optimized with naked-eye observations and SEM as 1.5 g salts and 5 wt.% PVA containing solution prepared at 90 degrees C and electrospun under 30 kV from a distance of 12-15 cm with a feeding rate of 5 mu l/min. Then the samples were subjected to a multi-step heat treatment under argon to remove the polymer and calcinate the nitrates into oxides which was designed based on thermal analyses and reaction kinetics calculations as 6 h at 230 degrees C, 8.5 h at 390 degrees C, 5 h at 465 degrees C, 80 h at 500 degrees C and 10 h at 505 degrees C, consecutively. The characterizations conducted in terms of structure, composition and crystallinity with XRD, XPS, EDX and SEM showed that it is possible to obtain MgaYbNdcZrdOx), (empirical) fibers with the same composition as WE43 in sub-millimeter sizes using this approach.Scientific and Technological Research Council of Turkey (TUBITAK) [117M177]The authors would like to thank The Scientific and Technological Research Council of Turkey (TUBITAK) for the scientific and financial support (Project No: 117M177). The authors would also like to acknowledge the contribution of AWAC (Academic Writing Advisory Center) of Atilim University to this study in linguistic terms
Novel poly(ε-caprolactone)/gelatin wound dressings prepared by emulsion electrospinning with controlled release capacity of Ketoprofen anti-inflammatory drug
No full text[EN] In the present study, a single and binary Ketoprofen-loaded mats of ultrathin fibers were developed by electrospinning and their physical properties and drug release capacity was analyzed. The single mat was prepared by solution electrospinning of poly(¿-caprolactone) (PCL) with Ketoprofen at a weight ratio of 5wt%. This Ketoprofen-containing PCL solution was also used as the oil phase in a 7:3 (wt/wt) emulsion with gelatin dissolved in acidified water. The resultant stable oil-in-water (O/W) emulsion of PCL-in-gelatin, also containing Ketoprofen at 5wt%, was electrospun to produce the binary mat. Cross-linking process was performed by means of glutaraldehyde vapor on the electrospun binary mat to prevent dissolution of the hydrophilic gelatin phase. The performed characterization indicated that Ketoprofen was successfully embedded in the single and binary electrospun mats, i.e. PCL and PCL/gelatin, and both mats showed high hydrophobicity but poor thermal resistance. In vitro release studies interestingly revealed that, in comparison to the single PCL electrospun mat, the binary PCL/gelatin mat significantly hindered Ketoprofen burst release and exhibited a sustained release capacity of the drug for up to 4days. In addition, the electrospun Ketoprofen-loaded mats showed enhanced attachment and proliferation of L929 mouse fibroblast cells, presenting the binary mat the highest cell growth yield due to its improved porosity. The here-developed electrospun materials clearly show a great deal of potential as novel wound dressings with an outstanding controlled capacity to release drugs.The authors would like to acknowledge the Spanish Ministry of Economy and Competitiveness (MINECO) project AGL2015-63855-C2-1-R for financial support. The authors would also like to thank the European Cooperation Science & Technology (COST) Action MP1206 for their Short Term Scientific Mission (STSM) at IATA-CSIC, Valencia, and the Academic Writing Advisory Center (AWAC) of Atilim University for English language revision.Basar, AO.; Castro, S.; Torres-Giner, S.; Lagaron, JM.; Turkoglu Sasmazel, H. (2017). Novel poly(e-caprolactone)/gelatin wound dressings prepared by emulsion electrospinning with controlled release capacity of Ketoprofen anti-inflammatory drug. Materials Science and Engineering C: Biomimetic materials, sensors and systems (Online). 81:459-468. https://doi.org/10.1016/j.msec.2017.08.025S4594688
In Vitro Evaluation of Tooth-Colored Yttria Stabilized Zirconia Ceramics
No full textPark, Jongee/0000-0003-1415-6906; Ozturk, Abdullah/0000-0002-1525-1561; Kaplan Akarsu, Melis/0000-0003-3040-8659; Turkoglu Sasmazel, Hilal/0000-0002-0254-4541; Basar, Ahmet Ozan/0000-0001-5301-6944Effects of MoCl3 and NiCl2, originally incorporated as coloring agent, on the cellular response of 3 mol% yttria stabilized zirconia (3Y-TZP) ceramics was investigated. MoCl3 and NiCl2-MoCl3 incorporated, tooth-colored 3Y-TZP ceramics were produced through cold isostatic pressing at 100 MPa followed by pressureless sintering at 1450 degrees C for 2 h. Aging was performed on the sintered ceramics using distilled water in a reactor at 134 degrees C at 2.3 bar pressure for 2 h. The phases developed during different stages of processing were identified by X-ray diffraction (XRD) analysis. In vitro cell culture studies were carried out using L929 fibroblast cell line. The cell viability and proliferation studies revealed that none of the specimens showed cytotoxicity with respect to coloring. Confocal laser scanning microscope (CLSM) analyses suggested that all of the specimens exhibited good in vitro cytocompatibility. Enhancement in cell attachment, adhesion, and proliferation was observed in all specimens via scanning electron microscope (SEM) analysis. Although the coloring process did not improve the proliferation performance of the aged specimens, the incorporation of transition metals enhanced the in vitro performance of 3Y-TZP ceramics.Middle East Technical University [BAP-07-02-2014-007-765]; Atilim University [ADP-1920002]This work was supported by Middle East Technical University [BAP-07-02-2014-007-765] and Atilim University [ADP-1920002]. We also thank the research facilities of VIT
Novel thin films deposited on electrospun PCL scaffolds by atmospheric pressure plasma jet for L929 fibroblast cell cultivation
No full textTurkoglu Sasmazel, Hilal/0000-0002-0254-4541; Arefi-Khonsari, Farzaneh/0000-0001-8123-5431This paper reports on the deposition of PCL homopolymers and poly epsilon-caprolactone-polyethylene glycol (PCL-PEG) copolymers by atmospheric pressure plasma jet (APPJ) onto electrospun PCL scaffolds for improving L929 fibroblast cell growth. Polymer deposited scaffolds showed better stability as well as lower CA as compared to those treated with APPJ in Ar alone used as the carrier gas to introduce the precursors due to the formation of polar groups generated during the plasma treatment, such as -OH and/or -COO. Average fiber and porosity sizes were calculated by using SEM photographs and the ImageJ Launcher Software program and higher values were observed for both PCL and PCL-PEG deposited scaffolds than the untreated electrospun PCL scaffolds. XPS analysis showed that C1s% content decreased for PCL deposited (from 82.4% to 71.0%) and PCL-PEG deposited (from 82.4% to 57.7%) and O1s% composition increased for PCL deposited (from 17.6% to 29.0%) and PCL-PEG deposited (from 17.6% to 42.3%) compared to the untreated one. XPS results proved more incorporation of oxygen moieties on the deposited surfaces than the untreated samples giving rise to more hydrophilic surfaces to the deposited ones. Standard in vitro MTT test, Giemsa staining, fluorescence and CLSM imaging techniques were used for the determination of cell viability, adhesion and proliferation. Cell culture experiments showed that PCL-PEG deposited electrospun PCL scaffolds had the most promising cell adhesion, proliferation and growth among the treated scaffolds. The increased average fiber diameter caused by deposition as well as oxygen containing polar groups formed on the surfaces due to the radicals present in the plasma atmosphere provided higher surface area and functionality, respectively, for cells to attach, yielding better biocompatibility performance.European Cooperation in Science and Technology (COST) Action MP1101 (Short Term Scientific Mission, STSM Turkey-France); Scientific and Technological Research Council of Turkey (TUBITAK) [112M043]The authors would like to acknowledge the European Cooperation in Science and Technology (COST) Action MP1101 (Short Term Scientific Mission, STSM Turkey-France 2015) and The Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 112M043) for their scientific and financial supports. The authors would also like to acknowledge the priceless contribution of AWAC (Academic Writing Advisory Center) of Atilim University to this study in linguistic term
Materials and Processes for Treatment of Microbiological Pollution in Water
No full textClean and safe water is vital for the life and health of human beings. However, there are still millions of people around the world with inadequate clean water sources. Microbiological pollution is one of the most concerned water pollutants and is the crucial cause of waterborne diseases like diarrhea, resulting in about two million deaths annually due to severe dehydration (WHO in Guidelines for drinking-water quality. Incorporating the first addendum, WHO, Geneva, 2017). It is critical to develop methods using advanced materials and process to mitigate contaminants from water resources. Production of safe water usually involves disinfection and decontamination processes. Conventional disinfection process, such as chlorination, is challenged by the formation of disinfection by-products. Furthermore, the presence of emerging pathogenic, that resist conventional water treatment techniques, raised the crucial necessity for emerging materials and techniques for treating water from microbiological pollution (Shannon et al. in Nature 452:301–310, 2008). This chapter describes bacterial, viral, and protozoal microbiological pollution in water supplies and the application of emerging materials and techniques to eliminate such contaminations. © 2021, Springer Nature Switzerland AG
Production of the Novel Fibrous Structure of Poly(ε-caprolactone)/Tri-calcium Phosphate/Hexagonal Boron Nitride Composites for Bone Tissue Engineering
No full textGunduz, Oguzhan/0000-0002-9427-7574; Ozbek, Burak/0000-0003-2133-001X; Ben-Nissan, Besim/0000-0003-0433-3593; Ekren, Nazmi/0000-0003-3530-9262; Gunduz, Oguzhan/0000-0002-4926-6489; Turkoglu Sasmazel, Hilal/0000-0002-0254-4541Nanofibrous composites of the poly(epsilon-caprolactone) (PCL), tricalcium phosphate (TCP), and hexagonal boron nitride (h-BN) with different compositions were manufactured by using an economical and non-complicated method called electrospinning. Produced fibrous structures showed no bead formation and had a clean surface. Characterization of the composites showed that particles were successfully mixed with polymer phase. High cell activity of SaOS-2 cells on the composites was observed with SEM images. In addition, fibrous scaffolds are biocompatible with human bone tissue and are highly degradable.Marmara University; BAPKO project [FEN-C-YLP-250416-0182]This study is funded by the Marmara University with the BAPKO project (FEN-C-YLP-250416-0182)
Poly(ε-caprolactone)/Chitosan Nanostructures for Cell Cultivation
No full textHybridization of synthetic poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop PCL/chitosan core-shell nanostructures for cell cultivation was aimed in this study. Coaxial electrospinning method was used for the fabrication of the nanostructures. The characterizations of the samples were done by X-ray photoelectron spectroscopy (XPS) analyses and mechanical tests. XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers. The cell culture studies, MTT assay and Confocal Laser Scanning Microscopy (CLSM), carried out with L929 ATCC CCL-1 mouse fibroblast cell line, proved the biocompatibility of all materials. The cell viability on the hybrid nanostructures was ~two times better then on tissue culture polystyrene (TCPS) because of its three dimensional (3D) extracellular matrix (ECM)-like structure compared to 2D flat surface of commercially cell compatible TCPS. The performance was ~two times and ~ten times better compared to single PCL and single chitosan, respectively, even though both fabricated similarly by electrospinning as non-woven fibrous structures, because were either too hydrophobic or too hydrophilic to maintain cell attachment points. © Springer Nature B.V. 2020.TUBITAK, (114 M872); Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITA
