81 research outputs found
Analysis of LRXs an their downstream components in cell growth regulation in Arabidopsis thaliana
No full textInfluence of surface defects in ZnO thin films on its biosensing response characteristic
No full textAl and Fe co-doped transparent conducting ZnO thin film for mediator-less biosensing application
No full textHighly c-axis oriented Al and Fe co-doped ZnO (ZAF) thin film is prepared by pulsed laser deposition. Fe introduces redox centre along with shallow donor level while Al doping enhances conductivity of ZnO, thus removing the requirement of both mediator and bottom conducting layer in bioelectrode. Model enzyme (glucose oxidase), was immobilized on surface of ZAF matrix. Cyclic voltammetry and photometric assay show that prepared bio-electrode is sensitive to glucose concentration with enhanced response of 0.18 μAmM-1cm-2 and low Km ∼ 2.01 mM. The results illustrate that ZAF is an attractive matrix for realization of miniaturized mediator-less solid state biosensor
A Modified Algorithm of Phase-Smoothed Pseudorange Based on Doppler Frequency Shift
No full textPhase-smoothed pseudorange algorithm is the commonly used pseudorange smoothing algorithm in navigation receivers. This paper analyses characteristics of weight factors of traditional smoothing algorithms and correlations in epochs. Proposes a modified smoothing algorithm based on Doppler frequency shift. This paper compares precisions of M-order, Hatch and this modified smoothing algorithm using original data from BeiDou receivers. Results show the modified algorithm obviously improves precisions of traditional smoothing algorithms, which can offer great reference for designation of pseudorange algorithm of navigation receivers.EICPCI-S(ISTP)[email protected]; [email protected]; [email protected]; [email protected]
A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application
No full textA novel hybrid matrix of zinc oxide-methylene blue (ZnO-MB) has been successfully developed for biosensing application. The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the composite. Glucose oxidase (GOx) was chosen as the model enzyme and effectively immobilized on the surface of hybrid ZnO-MB nanocomposite matrix. Electrochemical measurements were employed to study biosensing response of the GOx/ZnO-MB/ITO bioelectrode as a function of glucose concentration. The low oxidation potential (−0.23 V) of the hybrid bioelectrode, in a mediatorless electrolyte, makes it resistant against interference from other bio-molecules. The low value of Michaelis-Menten constant (2.65 mM) indicates that immobilized GOx retains its enzymatic activity significantly on the surface of nanocomposite hybrid matrix that results in an enhanced affinity towards its substrate (glucose). The ZnO-MB nanocomposite hybrid matrix, exhibiting enhanced sensing response (0.2 μAmM−1cm−2) with long shelf-life (>10 weeks), has potential for the realization of an integrated biosensing device
LRX- and FER-dependent extracellular sensing coordinates vacuolar size for cytosol homeostasis
No full textCellular elongation requires the defined coordination of intra- and extracellular processes. The vacuole is the biggest plant organelle and its dimension has a role in limiting cell expansion (Löfke et al., 2015; Scheuring et al., 2016). We reveal that the increase in vacuolar occupancy enables cellular elongation with relatively little enlargement of the cytosole. It remains, however, completely unknown how the vacuolar size is coordinated with other growth-relevant processes. Intriguingly, we show that extracellular constraints impact on the intracellular expansion of the vacuole. The underlying cell wall sensing mechanism requires the interaction of the extracellular leucine-rich repeat extensin (LRX) with the receptor-like kinase Feronia (FER). Our data suggests that LRX links the plasma membrane localised FER with the cell wall, allowing this module to jointly sense and convey extracellular signals to the underlying cell. This mechanism coordinates cell wall acidification/loosening with the increase in vacuolar size, contributing cytosol homeostasis during plant cell expansion.</jats:p
Temperature dependent optical properties of (002) oriented ZnO thin film using surface plasmon resonance
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