1,333 research outputs found
HPS5-SQ-mediated autophagy envelops mitochondria under oxidative stress and kills invading bacteria.
No full text(A) STEC were fixed and subjected to immunofluorescence analysis to detect LC3 (pink) and mitochondrial fluorescence (red and green). Scale bar: 10 μm. (B) Immunofluorescence of control, HPS5-SQ, and HPS5-SQ+NAC (5 mM for the final 1 h) infected STEC stained for mROS and LC3B. The white triangle refers to HPS5-SQ (blue). Scale bar: 10 μm. (C) STEC were treated with 3-MA for the final 3 h or Baf-A1 for the final 4 h, quantifying the invading HPS5-SQ in the infection of HPS5-SQ for 6 and 12 h. Data shown are means ± SEM; ** P P < 0.01. (one-way ANOVA).</p
HPS5-SQ-mediated autophagy degrades Claudin-1 covering mitochondria in STEC.
No full text(A) The expression of the mCherry-FIS1 and EGFP-Claudin-1 in STEC. Immunofluorescence microscopy images detected the subcellular localization of mitochondria and Claudin-1. Scale bars: 5 μm. (B) Western blot showing Claudin-1 and Parkin expression in HPS5-SQ-infected cells or treated with mitophagy activator: CCCP for 6 and 12 h. (C) Quantification of Claudin-1 and Parkin. Data shown are means ± SEM; * P P < 0.001. (one-way ANOVA). (D) STEC expressing mCherry-FIS1 and EGFP-Claudin-1 were control-treated or infected with HPS5-SQ (MOI of 100) and treated with CCCP for 12 h as a positive control. Immunofluorescence microscopy images detected the Claudin-1 and mitochondria. Scale bar: 5 μm. (E) The expression of the mCherry-FIS1 and EGFP-Claudin-1 in STEC were treated with Baf-A1 for the final 4 h or untreated with Baf-A1, then CCCP was added to STEC pretreated with Baf-A1 for 12 h. Immunofluorescence microscopy images detected the subcellular localization of mitochondria and Claudin-1 in STEC pretreated with Triton-100 for more than 12 h. Scale bars: 5 μm.</p
HPS5-SQ-mediated autophagy involves the decline of porcine respiratory epithelial Claudin-1.
No full text(A) The mROS staining of piglets’ lung tissue section of the autopsy showed the mROS (red) and the nucleus (blue). Scale bars: 1000 μm, 100 μm. (B) Pictures were marked by @ and & in Fig 6A, and immunofluorescence microscopy detected the Claudin-1 (pink) and DAPI (blue) in piglets lung tissue. The picture was marked by ii and iv in Fig 6A, and immunofluorescence microscopy detected the localization of mROS(red), LC3(green), and DAPI (blue) in healthy piglets lung tissue infected with HPS5-SQ. Scale bars: 200 μm, 50 μm, 20 μm.</p
Designing role-based view for object-relational databases
No full textIn a federated database system, a view mechanism is crucial since it is used to define exportable subsets of data ; to perform a virtual restructuring d ataset; and to construct the integrated schema. The view service in federated databa se systems must be capable of retaining as much semantic information as possible. The object-oriented ( 0 - 0 ) model was considered the suitable canonical data model since it meets the original criteria for canonical model selection. However, with the emergence of stronger object-relational (0 -R ) model, the re is a clear argument for using an 0 - R canonical model in the federation. Hence, research should now focus on th e development of semantically powerful view mechanism for th e newer model. Meanwhile, the availability of real 0 -R technologies offers researchers the opportunity to develop different forms of view mechanisms.
The concept of roles has been widely studied in 0 - 0 modelling and development. The role model represents some characteristics that the traditional 0-0 model lacked, such as object migration, multiple occurrences and context-dependent access. While many forms of 0-0 views were designed for the 0-0 canonical model, one option was to extend the 0-0 model to incorporate a role model. In a role model, the real entity is modelled in the form of a role rather than an object. An object represents the permanent properties of an entity is a root object; and an object represents the temporary properties of an entity is a role object.
The contribution of this research is to design a view system that employees the concept of roles for the 0 -R canonical model in a federated database system. In this thesis, an examination of the current 0 -R metamodel is provided first in order to provide an environment for recognising the roleview metadata and measuring the view performance; then a Roleview Definition Language (RDL) is introduced, along with the semantics for defining virtual classes and generating virtua l extents; finally, a working prototype is provided to prove th e role-based view system is implementable and the syntax is semantically correct
Numerical study of dynamic phase transitions in 2-D with a relaxed scheme
No full texthttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000189123800083&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Mathematics, AppliedPhysics, MathematicalCPCI-S(ISTP)
Asia region population projections : 1990-91 edition
No full textAlmost half the worlds population lives in Asia. This proportion is expected to decline to 40 percent by the end of the next century, mainly because of slowing growth in China. Other countries will continue to grow rapidly, and India, which adds more people every year than any other country, is project to surpass China in total population. Recent contraceptive prevalence surveys in several countries in the region show increasing proportions of couples using birth control. Fertility in these countries, mostly in Southeast Asia, has consequently declined rapidly. Population growth rates started to drop in many countries in the region in the past decade, but the momentum built in to the age structures of the populations will ensure continued population growth for many decades. Other countries in the region are lagging in fertility decline, and their populations will continue to grow at high rates. Infant and child mortality are the lowest in countries where fertility has declined.Earth Sciences&GIS,Demographics,Health Indicators,Health Information&Communications Technologies,
HPS5-SQ infection degrades cytoplasm Claudin-1 in STEC.
No full text(A) STEC were infected with control or HPS5-SQ (MOI 100) for 12 h and 24 h in media. STEC were fixed and subjected to immunofluorescence analysis to detect Claudin-1 (green). Scale bar: 10 μm. (B) STEC were control-treated or infected by HPS5-SQ at an MOI of 100, and whole-cell extracts were prepared from control-infected and HPS5-SQ-infected cells at 12 and 24 hpi. (C) Quantification of cytoplasm Claudin-1 and cytomembrane Claudin-1. Data shown are means ± SEM; ** P P P t-tests). (E) STEC were either control treated or infected by HPS5-SQ at an MOI of 100. mRNA levels of Claudin-1 were detected using qRT-PCR.</p
Sequence length distribution in Wm/Wm, Wm/BG and Wm/Sq.
No full text<p>Sequence length distribution in Wm/Wm, Wm/BG and Wm/Sq.</p
Africa region population projections : 1990-91
No full textAs recently as the mid-1970s, the Africa region had a smaller population than the Asia, the Latin American and the Caribbean, or the Europe, Middle East, and North Africa regions. Explosive population growth of more than 3 percent per year, projected to decline only gradually, will make Africa the second largest region by 2005. Its share of the world's population will increase from less than 10 percent now to 20 percent in the middle of the next century and to 25 percent when stationarity is finally reached. Vital rates vary relatively little among the subregions of sub-Saharan Africa. Fertility is uniformly high, with the total fertility rate higher than 6 children per woman. Linked with high fertility are high infant mortality rates, which are above 100 per thousand births for subregions. A few countries - Botswana, Zimbabwe, and Kenya - are leading the way in the African fertility transition. Recent fertility surveys in these countries show an increase in the use of contraceptives and the first evidence of fertility decline. It is assumed in the projections that this trend will spread to other countries. Most African governments now report their country's population growth rates as too high.Demographics,Health Indicators,Health Information&Communications Technologies,,Earth Sciences&GIS
Reads abundance of various classification of small RNAs in Wm/Wm, Wm/BG and Wm/Sq.
No full text<p>Reads abundance of various classification of small RNAs in Wm/Wm, Wm/BG and Wm/Sq.</p
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