1,720,972 research outputs found
Biodiversità degli endofiti fungini della vite e sviluppo di un metodo molecolare per il loro fingerprinting in piante sane, infette e recovered da Legno nero (LN)
Full text linkNel presente lavoro di tesi si è voluto studiare la comunità endofitica fungina della vite mediante l’utilizzo integrato di metodiche di micologia classica, molecolari e di “fingerprinting”. Questo studio ha voluto inoltre stabilire una possibile correlazione tra la comunità endofitica fungina caratterizzata e la remissione spontanea dei sintomi riscontrata in viti infette dal fitoplasma del Legno Ner
CHARACTERIZATION OF FUNGAL ENDOPHYTIC COMMUNITY OF THE GRAPEVINE BY CULTURE-DEPENDENT AND CULTURE-INDEPENDENT METHODS.
No full textDevelopment of a molecular approach to describe the diversity of fungal endophytes in either phytoplasma infected, recovered or healthy grapevines
No full textACTIVITY OF THE ENDOPHYTIC FUNGUS EPICOCCUM NIGRUM LINK AGAINST ‘CANDIDATUS PHYTOPLASMA MALI’ IN CATHARANTHUS ROSEUS (L.) G. DON PLANTS
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DNA-dependent detection of the grapevine fungal endophytes Aureobasidium pullulans and Epicoccum nigrum
No full textAureobasidium pullulans and Epicoccum nigrum are frequently reported as endophytes of various
crops, including grapevine (Vitis vinifera). Because of their potential role as biological control
agents against grapevine pathogens, we examined the occurrence of A. pullulans and E.
nigrum in two grapevine varieties (Merlot and Prosecco) in Italian vineyards where spontaneous
recovery from phytoplasma disease is recurrent. Species-specific primers for A. pullulans and
two genetically distinct strains of E. nigrum were designed in variable regions of ITS1 and ITS2.
Primer specificity was confirmed by polymerase chain reaction using purified DNA from other
fungal endophytes that are usually encountered during isolation attempts from grapevine tissues
and from several other strains of A. pullulans and E. nigrum isolated from other sources. In order
to determine the occurrence of the two endophytes in grapevine plants, DNA was extracted from
shoots of 44 grapevines collected in six vineyards from different localities of northeast Italy.
Both endophytes were detected and their identity was confirmed by restriction fragment length
polymorphism (RFLP) patterns obtained from reference strains. RFLP analyses confirmed the
presence of two E. nigrum strains belonging to different RFLP groups in grapevine. The molecular
methods described allowed a sensitive, specific, and reliable identification of the two endophytes
in grapevine
Laser microdissection of grapevine leaf phloem infected by stolbur reveals site-specific gene responses associated to sucrose transport and metabolism.
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