68 research outputs found
Development of a plant-based vaccine against malaria
No full textMalaria is the most prevalent tropical human disease reported worldwide, caused by protozoan parasites. Half of the world's population is at risk of malaria, and more than 200 million new cases are reported annually. Currently, there are no licensed vaccines available for use. Therefore, there is a vital need for developing an effective and reliable anti-malaria vaccine ideally protecting different parasitic infection stages comprising different antigens that generate appropriate cell-mediated antibody responses of the parasite presentation. Plant-based vaccines serve as novel platforms for developing safe, reliable, and affordable treatments.
In this study, a Malchloroplast candidate vaccine is designed, comprised of segments of AMA-1 and MSP-1 proteins along with the GK1 peptide form Teania solium as adjuvant, and expressed in tobacco chloroplasts. Transplastomic tobacco lines have been generated using biolistic transformation, and these are confirmed to carry the synthetic gene construct. The synthetic GK1 peptide is confirmed to be expressed using RT-PCR and Western blots, and detected by RP-HPLC at levels of up to 6 µg g-1 dry weight of tobacco leaf tissue. The plant-derived Malchloroplast candidate vaccine components have been recognized by antibodies in Plasmodium falciparum Malaria patients, and has elicited specific antibodies in subcutaneously immunized BALB/c mice.
Additionally, a peptide-based vaccine, Mvac, targeting the MSP1 and AMA1 antigens was evaluated in combination with different adjuvants in an oral and subcutaneous immunization scheme applied to BALB/c mice. Adjuvants tested were plant DNA, pectin, β-subunit of cholera toxin and the GK1 peptide from T. solium. Neither plant DNA nor pectin enhanced the humoral response induced against the Mvac components. While, GK1 peptide had exerted adjuvant effects in terms of the systemic IgG responses induced against the AMA1 peptide, although pectin enhanced the IgA intestinal secretion against both MSP1 and AMA1 antigens.
Overall our findings suggest that a multi-component plant-based vaccine against malaria expressing AMA1 and MSP1 antigens, and the GK1 peptide has the potential to serve as a viable and promising low-cost vaccine. As well as oral administration of a vaccine with GK1 peptide has a promising immunogenic effects, proposing that a plant-based vaccine against Malaria administered orally can be effective.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-12-01The student, Evelia Milan Noris, accepted the attached license on 2015-12-01 at 17:54.The student, Evelia Milan Noris, submitted this Dissertation for approval on 2015-12-01 at 18:06.This Dissertation was approved for publication on 2015-12-03 at 08:51.DSpace SAF Submission Ingestion Package generated from Vireo submission #8903 on 2016-03-02 at 14:13:43Made available in DSpace on 2016-03-02T21:06:54Z (GMT). No. of bitstreams: 2
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Modelling and Assessment of Restoration in Electrical Power Systems with High Penetration of Power Electronic Converters
No full textPower outages can damage severely critical infrastructures such as telecommunication networks, financial services, water supplies and hospitals and completely shutting down production at companies. While most power blackouts usually last from some minutes to few hours, some can last days or even weeks. Furthermore, it is likely that these kinds of events would become more recurrent due to the stranded expansion of aged Electrical Power System (EPS) infrastructures in Europe for coping with the increased societal and environmental pressure for massive deployment of adopting Renewable Energy Source (RES)-based generation, which is known for having a variable characteristic. It is expected that RES will become the dominant factor in the power grid and will gradually replace Conventional Synchronous Generation Units (CGU), and it is known that Offshore Wind Farms (OffWF) are more suitable for large-scale generation applications. However, RES, including Wind Farms (WF), are not well aligned to work with EPS that were designed fifty - to - sixty years ago, due to reduced inertia, reduced short-circuit power, and limited control capabilities that imply the integration of RES. Moreover, more grids will contain more renewables, and thus more risks of outages could arise as one fault can trigger another one as a domino effect, deriving in widespread disruptions, including regional blackouts. In this context, the contemporary control systems that regulate WF inject power to the grid via Power-Electronic Interfaces (PEI), and their schemes are designed to not interfere actively with the safe and secure regulation of a large-scaled EPS. On the contrary, they are just limited to inject a predetermined power injection setpoint with a current-injection control method that assumes all the time this power has load demand to go (grid-following control). However, this is not the case when unplanned islanding or an outage arises in an EPS, as the WF currently do not possess a control system that regulates the frequency of an island. This MSc Thesis Project presents the design, implementation and testing of a control system attached to type-4 Wind Turbines (WT) that can manage and tightly ensure the load and generation balance during any circumstance, including a massive blackout. This control method can successfully regulate voltage, reactive power and frequency, which can be adapted automatically to the real-time conditions of the grid. The scheme takes the grid-following control approach as a starting point, which was modified in order to have Grid-Forming and Black-Start capabilities. The proposed new control approach for grid-forming and black-start design was implemented in DIgSILENT PowerFactory 2018, where a total of seventeen large-scaled type-4 WF are located into a three-area EPS. The Permanent-Magnet Synchronous Generator (PMSG) WF containing the proposed grid-forming control system are accompanied by Hydro, Nuclear and Thermal Plants, accounting CGU. Additionally, the three-area EPS also contains two HVDC Transmission Systems composed each by two Voltage-Source Converters (VSC), which also have similar grid-forming and black-start capabilities, and seven Battery-Energy Storage Systems (BESS), which give auxiliary power to the Black-Start Units and frequency support to the areas with loss of generation. The WF, BESS and the HVDC stations are interfaced via modelled Modular Multi-level Converter (MMC) controlled voltage sources. The proposed grid-forming and black-start capabilities of the three-area EPS were tested with several EMT simulations reproducing severe short-circuit faults followed by a loss-of-generation scenario, the blocking of the HVDC converter stations and a massive relay protection program, resulting in the full outage and isolation of the area responsible for the largest power supply in the three-area EPS. After the blackout, the three-area EPS performed a Restoration plan, from the generation resetting and the reconnection of lines, transformers, etc., to the final (cold) load pick-up stage. In order to evaluate the advantage of using utility-scaled WF with grid-forming controllers to execute a conjunct Black-Start and Restoration plan, two operational scenarios were performed: one with 90% wind power share and another without any participation of wind power. As a consequence of the implementation of the proposed grid-forming control systems, the simulation results endorse that an EPS with 90% wind power share can steer a Black-Start and Restoration operation when required.Electrical Engineering | Electrical Power Engineerin
RTDS/RSCAD Type-3 Doubly-Fed Induction Wind Turbine Generator Model: Internship Report
No full textIn the future, it is expected that the European Power Grid will be integrated with several sources of renewable energy producers (mainly wind/solar), alternating their maximum/minimum production periods per year. All involved parts of the European Power System (generation and consumption units) will increasingly be connected to the network utilizing power electronic devices. This will lead to technical challenges due to a dramatic reduction of rotational inertia in the system (specifically, due to the phase-out of conventional generation units with large synchronous machines) to guarantee, despite these potential inertia issues, stability at 50 Hz. The MIGRATE Project has the goal of developing solutions to ensure grid stability, control and security and quality of supply. In this manner, a Type-3 Doubly-Fed Induction Generator (DFIG) Wind Turbine (WT) Model was implemented in the Real-Time Digital System (RTDS) Power System Simulator to help the German Transmission System Operator (TSO) TenneT GmbH to analyze such phenomena with a real-time simulation model. The wind generator model provides a representation of a complex electro-mechanical system and portraits the controls, electrical and mechanical dynamics of the wind generators for conducting Electromagnetic Transient (EMT) simulations. First, it was learned how to set up simple power systems models in RTDS. Then, a translation/resemblance of an already-existing wind generator model elaborated in Manitoba HVDC Research Centre's PSCAD software tool platform was done into the RTDS domain. The final task was to validate the RTDS models, by comparing the time responses obtained in both PSCAD and RTDS. Overall, the expected goal of the internship, which was to synthesize all the acquired data from the PSCAD models to translate and migrate such models into RSCAD models ready for the use in the RTDS, was achieved.https://www.h2020-migrate.eu
OPINIA PUBLICĂ ON-LINE – DERIVATĂ A NOILOR MEDIA
No full textÎn articol este tratat fenomenul opiniei publice în condiţiile ascensiunii noilor media. Pornind de la viziunile exprimate în lucrările unor cercetători din domeniu, ca Patrick Champagne, Pippa Noris, Camelia Beciu, Paul Dobrescu, Rémy Rieffel ş.a., autorul abordează conceptul opiniei publice, formulează definiţia opiniei publice on-line şi evidenţiază etapele formării acesteia: informarea cetăţenilor prin intermediul diverselor forme ale new media, realizarea deliberării publice în spaţiul public virtual, procesul consultării publice on-line. De asemenea, sunt relevate rolul liderilor de opinie în procesul de difuzare a informaţiei publicului în mediul on-line şi importanţa noilor tehnologii informaţionale pentru desfăşurarea unui dialog direct şi transparent între liderii politici şi cetăţeni.PUBLIC OPINION ON-LINE – ARISING OUT OF NEW MEDIA The article is based on the analysis of several scientific papers signed by Patrick Champagne, Pippa Noris, Camelia Beciu, Paul Dobrescu, Rémy Rieffel, etc. The approach of the concept of public opinion allows the author to formulate the definition of online public opinion and described the three basic stages of its formation and expression: information to citizens via certain forms of new media, realization of public deliberations in digital public space and the process of online public consultation. Also, was mentioned the role of opinion leaders in the process of disseminating information to the public in the online environment and the importance of new technologies for the establishment of direct and transparent dialogue between political leaders and citizens.</p
Trends and Issues of Ethnoscience Research from 2008 to 2023: A Bibliometric Analysis
No full textThis paper aims to analyze research trends on ethnoscience using bibliometric analysis from 2008-2023. The research sample consisted of 153 documents obtained from the Scopus database. The results of the study show that the distribution of publication frequency reaches its peak in 2021 with 32 articles identified. The distribution of research themes consists of 4 primary clusters and 35 secondary clusters. The ethnoscience research area is dominated by social science research (30.2%). The country with the best documents shows that Indonesia is ranked first as the most productive country in publishing on ethnoscience with 74 identified documents. The United States released second place with 28 documents, third Brazil with 10 documents, fourth Canada with 9 documents, and fifth France, Germany, Italy and the Russian Federation with 5 documents each. Institutions that contributed the most came from Indonesia, Universitas Negeri Semarang 22 papers 33.66%, University of Alberta 9 papers 13.77%, Universitas Negeri Surabaya 7 papers 10.71, Universitas Negeri Padang 7 papers 7.65%. The best author with the highest number of citations is Dahdouh. Meanwhile, if we look at the number of documents published by the author, Sudarmin has 10 documents with a contribution of 15.3%.
 
Adaptation of younger preschool children in a pre-school educational institution
No full textBakalaura darba tēma ir „Jaunākā pirmsskolas vecuma bērnu adaptācija pirmsskolas izglītības iestādē.” Darba autore to izvēlējās, lai izpētītu, kā veicināt jaunākā pirmsskolas vecuma bērnu adaptāciju pirmsskolā. Bakalaura darba pētījuma mērķis ir izpētīt jaunākā pirmsskolas vecuma bērnu veiksmīgas adaptācijas nosacījumus pirmsskolas izglītības iestādē. Autore izvirzīja pētījuma hipotēzi - jaunākā pirmsskolas vecuma bērnu adaptācija noris veiksmīgāk, ja pirmsskolas izglītības iestādē tiek rīkoti adaptāciju veicinoši pasākumi un adaptācijas procesā tiek iesaistīti vecāki. Lai sasniegtu izvirzīto mērķi un pierādītu hipotēzi, tika izvirzīti uzdevumi iepazīties un analizēt teorētisko literatūru par adaptācijas procesu pirmsskolā, iepazīties ar 1,5 - 3 gadīgo bērnu vecumposma īpatnībām, apkopot un raksturot galvenos sekmīgas adaptācijas priekšnosacījumus pirmsskolas izglītības iestādē, veikt pētījumu un apkopot un analizēt pētījumā iegūtos datus. Pētījuma gaitā bakalaura darba autore nonāca pie secinājuma, ka jaunākā pirmsskolas vecuma bērnu adaptācija noris veiksmīgāk, ja pirmsskolas izglītības iestādē tiek rīkoti bērnu adaptāciju veicinoši pasākumi un adaptācijas procesā tiek iesaistīti vecākiAnotation The subject of the bachelor's work is “Adapting children of the youngest preschool age to a preschool education institution.” The author chose this to explore how to encourage the adaptation of children of the youngest preschool age to preprimary school. The aim of the bachelor's work study is to explore the conditions for the successful adaptation of children of the youngest preprimary age in a preprimary education institute. The author put forward a study hypothesis: the latest adaptation of children of preschool age is more successful when adaptation enhancing measures are organized in a preprimary education institution and parents are involved in the adaptation process. In order to achieve the objective and demonstrate the hypothesis, the objectives were to familiarize and analyze theoretical literature on the preschool adaptation process, to familiarise themselves with the characteristics of the age of 1.5 - 3year children, to collect and describe the key conditions for successful adaptation in the preschool education institution, to carry out a study and to collect and analyze the data obtained from the study. In the course of the study, the bachelor's author came to the conclusion that the latest adaptation of children of preschool age is more successful when child adaptation measures are organized in a pre-primary education institution and parents are involved in the adaptation process
Problems of interrogation of minor victim and their solutions
No full textDarba tēma: Nepilngadīgā cietušā pratināšanas problēmas un risinājumi. Bakalaura darba mērķis ir noskaidrot nepilngadīgā cietušā jēdziena tvērumu šī darba ietvaros un tā procesuālo statusu kriminālprocesā, kā arī izpētīt vispārējos aspektus, kas tiek attiecināti uz nepilngadīgā cietušā pratināšanu, kuros nav saskatāmas problēmas un pēc tam padziļinātāk izpētīt šīs problēmas. Pirms darba veikšanas autore uzskatīja, ka nepilngadīgā cietušā pratināšanā vislielākās problēmas ir ar speciālo telpu nodrošināšanu, nepieciešamo zināšanu apguvi. Šī hipotēze daļēji apstiprinājās saistībā ar to, ka šobrīd noris dažādi pasākumi, lai tiktu pilnveidots šis institūts. Rezultātā autore sniedz secinājumus un nepieciešamos risinājumus, lai nepilngadīgā cietušā pratināšana būtu vēl efektīvāka.Subject: Problems of interrogation of minor victim and their solutions. The purpose of the Bachelor's work is to clarify the scope of the concept of a victim of a minor within the scope of this work and its procedural status in criminal proceedings, as well as to explore the general aspects that apply to the interrogation of a victim of a minor, in which there are no obvious problems and then to examine these problems in greater depth. Prior to the performance of the work, the author considered that the greatest problems in the interrogation of the victim of a minor are the provision of special premises, acquisition of the necessary knowledge. This hypothesis author confirmed in part by the fact that various measures are currently being taken to develop this institute. As a result, the author provides conclusions and the necessary solutions to make interrogation of the victim of a minor even more effective
Cualificaciones y competencias profesionales del trabajo social en Ecuador:comparación con otros países de su entorno
No full textTesis inédita de la Universidad Complutense de Madrid, Facultad de Trabajo Social, Departamento de Trabajo Social y Servicios Sociales, leída el 22-11-2019Respecto a la decisión de desarrollar este tema, la autora destaca las transformaciones de las relaciones sociales por el desarrollo de la globalización que a pasos agigantados provoca avances en la ciencia, saberes, comunicación, tecnología lo que promueve que los poderes del estado, actores sociales, instituciones se preocupen por estar a la vanguardia de la innovación para proponer cambios que generen sobre todo en la educación la reestructuración de la oferta académica a nivel de los diferentes niveles de educación en especial en la educación superior, se implementen propuestas curriculares acordes con la innovación científica y tecnológica para el logro de resultados de aprendizaje que promuevan la calidad en la formación de los profesionales. Otro de los aspectos que hacen pertinente el trabajo investigativo son los postulados de la carta magna del Ecuador (2008) que reorienta y prioriza la intervención del Estado en los programas sociales, en el mejoramiento de la calidad educativa de todo el Sistema Nacional de Educación, en la capacitación, innovación, evaluación y desempeño de los docentes, en la formación de los profesionales que egresan de las diversas universidades del país, en la propuesta y diseño de los currículos con el enfoque general de competencias, en la oportunidad de becas para la innovación de conocimientos en las mejores universidades a nivel nacional, regional y mundial...Regarding the decision to develop this theme, the author highlights transformations of social relations for the development of globalization which rapidly leads to advances in science, technology, knowledge, communication that promotes that the powers of the State, social actors, institutions worry about being at the forefront of innovation to propose changes that generate primarily in the education restructuring of the academic offerings at the level of the different levels of education in particular in higher education, proposed curricular chords with scientific and technological innovation for the achievement of learning outcomes that promote quality in the training of professionals.Another aspect that makes relevant the research work are the tenets of the Constitution of Ecuador (2008) that reorients and prioritizes the intervention of the State in social programmers, the improvement of the educational quality of the entire national system education, training, innovation, evaluation and performance of teachers, in the training of professionals who graduate from different universities of the country, in the proposal and design of curricula with the general approach of competences, in the opportunity scholarships for the innovation of knowledge at the best universities to national, regional and global levels...Depto. de Trabajo Social y Servicios SocialesFac. de Trabajo SocialTRUEunpu
Utilización de elementos captanieblas, destinados al acondicionamiento pasivo térmico en el Nuevo Aeropuerto Internacional de Huanchaco
No full textRESUMEN
El presente estudio tiene como planteamiento general la relación de las variables Elementos captanieblas y el Acondicionamiento pasivo térmico, orientadas al diseño de una envolvente en un Nuevo Aeropuerto Internacional de Huanchaco.
El autor desarrolla el informe en cinco capítulos:
El capítulo 1, comprende la situación actual del lugar de emplazamiento del aeropuerto, fundamentando la necesidad de su construcción en la ciudad. Asimismo, abarca las causas del desarrollo de las variables planteadas y el respectivo marco teórico de cada una. De esta manera se establece una relación entre el equipamiento y las variables para plantear los objetivos.
En el capítulo 2, se formula la hipótesis, se definen las variables y aquellos términos propios del sector aeroportuario, asimismo según las bases teóricas se establecen las dimensiones e indicadores de las variables en un cuadro de operacionalización. Dentro de las dimensiones se encuentran el diseño y eficiencia, sistema constructivo, estrategia de masa térmica, estrategias de enfriamiento pasivo y estrategias de calentamiento pasivo.
El capítulo 3, se expone el tipo de diseño de investigación y los casos pertinentes a las dimensiones e indicadores: a) Caso 1: Torre de Niebla para el Desarrollo Agrícola o Coastal Fog Tower. b) Caso 2: Casa Atrapanieblas o Fogcatcher House. c) Caso 3: Artefacto macrodiamante. d) Caso 4: Estación de base temporal y permanente. e) Caso 5: Casa del agua f) Caso 6: Espacios abiertos de la Exposición Universal de 1992. g) Caso 7: Aeropuerto Jewel Shangi, Singapur. h) Edificio Secretaría Ministerial de Obras Públicas.
El capítulo 4, se analiza estos casos y en función a ellos se concluyen en lineamientos de diseño para las variables, las cuales serán punto de partida en el planteamiento de diseño arquitectónico.
Finalmente, en el capítulo 5, se desarrolla la aplicación de la investigación de las variables en la infraestructura aeroportuaria.ABSTRACT
The present study has as a general approach the relation of the variables capstanieblas elements and the passive conditioning, oriented to the design of an envelope in a New International Airport of Huanchaco.
The author develops the report in five chapters:
Chapter 1, includes the current situation of the location of the airport, substantiating the need for its construction in the city. It also covers the causes of the development of the variables proposed and the respective theoretical framework of each one. In this way, a relationship is established between the equipment and the variables to set the objectives.
In chapter 2, the hypothesis is formulated, the variables and those terms specific to the airport sector are defined, also according to the theoretical bases, the dimensions and indicators of the variables are established in an operationalization table. Within the dimensions are the design and efficiency, construction system, thermal mass strategy, passive cooling strategies and passive heating strategies.
Chapter 3, the type of research design and cases relevant to the dimensions and indicators are exposed: a) Case 1: Fog Tower for Agricultural Development or Coastal Fog Tower. b) Case 2: Fogcatcher House or Fogcatcher House. c) Case 3: Macrodiamante artifact. d) Case 4: Temporary and permanent base station. e) Case 5: House of water f) Case 6: Open spaces of the 1992 Universal Exhibition. g) Case 7: Jewel Shangi Airport, Singapore. h) Ministry of Public Works Building.
Chapter 4, these cases are analyzed and according to them are concluded in design guidelines for the variables, which will be the starting point in the approach to architectural design.
Finally, in chapter 5, the application of the investigation of the variables in the airport infrastructure is developed
Primed T Cell Responses to Chemokines Are Regulated by the Immunoglobulin-Like Molecule CD31
No full textPMCID: PMC3378580This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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