494 research outputs found
'To encounter the unexpected' : Kate Fagan in conversation with Miro Bilbrough
No full textOn 26 March 2021, in a window between lockdowns, author and filmmaker Miro Bilbrough and I met to discuss her free-wheeling memoir, In the Time of the Manaroans (Ultimo Press, 2021). The conversation transcribed here was shared with a wide audience via Zoom as part of the online ‘Room to Listen’ seminar series, hosted in Parramatta by the Writing and Society Research Centre at Western Sydney University. I now invite you to read, listen and absorb Miro’s flair for poetic storying
Miro Remo, The Film Author
Get PDFDizertační práce se komplexně zabývá filmovou tvorbou slovenského režiséra Mira Rema. Výzkum vycházející ze stěžejní úvahy o Removi jakožto tvůrci s osobitým autorským rukopisem stojí na několika opěrných bodech. V jedné z rovin se zaměřuje na témata a postavy, kterými se Remo ve svých filmech zabývá, a s ohledem na to reflektuje, skrze jaké režijní metody, stylové a narativní postupy či prvky filmové řeči je zpřítomňuje filmovému publiku (včetně toho, do jaké míry se opakují či variují). Vedle takto zaměřených analýz jednotlivých děl je pozornost koncentrována i na okolnosti jejich vzniku a dílčí fáze jejich produkce, tzn. na vývoj, výrobu, postprodukci a distribuci. Velký prostor je rovněž věnován kritickému diskurzu a na základě zevrubných rešerší je mapováno, jakým způsobem se projekty Mira Rema diskutovaly v médiích, jaké kauzy vyvolávaly nebo jak rezonovaly u filmové kritiky a v rámci festivalové scény. Výzkum zahrnuje i sérii polostrukturovaných rozhovorů: jak se samotným Mirem Remem, tak i s jeho blízkými spolupracovníky ze všech hlavních štábních profesí ? scenáristou a dramaturgem Jurem Šlaukou, kreativním producentem Markem Kučerou, kameramanem Jarem Vaľkem, střihačem Markem Kráľovským a zvukařem Lukášem Kasprzykem.The thesis deals comprehensively with the film work of the Slovak director Miro Remo. The research is based on the central reflection of Remo as a film author who possesses distinctive handwriting. On one level, it focuses on the themes and characters that Remo deals with. The theses also reflects on the directorial methods, stylistic and narrative approaches or elements of film language through which the director communicates them to the film audience (including the extent to which they are repeated or varied). In addition to such focused analyses of individual works, attention is also given to the circumstances of of their creation as well as the sub-phases of their production, i.e. their development, preproduction, shooting, post-production and distribution. A great deal of the work is also dedicated to critical discourse and ? based on extensive research ? it is mapped how Remo's projects were discussed in the media, what kind of controversies they provoked or how they resonated with film critics or within the festival scene. The research includes a series of semi-structured interviews: with Miro Remo himself, as well as with his close collaborators from several professions ? screenwriter and script consultant Juro Šlauka, creative producer Marek Kučera, DOP Jaro Vaľko, editor Marek Kráľovský and sound designer Lukáš Kasprzyk.Ateliér Audioviz
Vimar Is a Novel Regulator of Mitochondrial Fission through Miro.
No full textAs fundamental processes in mitochondrial dynamics, mitochondrial fusion, fission and transport are regulated by several core components, including Miro. As an atypical Rho-like small GTPase with high molecular mass, the exchange of GDP/GTP in Miro may require assistance from a guanine nucleotide exchange factor (GEF). However, the GEF for Miro has not been identified. While studying mitochondrial morphology in Drosophila, we incidentally observed that the loss of vimar, a gene encoding an atypical GEF, enhanced mitochondrial fission under normal physiological conditions. Because Vimar could co-immunoprecipitate with Miro in vitro, we speculated that Vimar might be the GEF of Miro. In support of this hypothesis, a loss-of-function (LOF) vimar mutant rescued mitochondrial enlargement induced by a gain-of-function (GOF) Miro transgene; whereas a GOF vimar transgene enhanced Miro function. In addition, vimar lost its effect under the expression of a constitutively GTP-bound or GDP-bound Miro mutant background. These results indicate a genetic dependence of vimar on Miro. Moreover, we found that mitochondrial fission played a functional role in high-calcium induced necrosis, and a LOF vimar mutant rescued the mitochondrial fission defect and cell death. This result can also be explained by vimar's function through Miro, because Miro's effect on mitochondrial morphology is altered upon binding with calcium. In addition, a PINK1 mutant, which induced mitochondrial enlargement and had been considered as a Drosophila model of Parkinson's disease (PD), caused fly muscle defects, and the loss of vimar could rescue these defects. Furthermore, we found that the mammalian homolog of Vimar, RAP1GDS1, played a similar role in regulating mitochondrial morphology, suggesting a functional conservation of this GEF member. The Miro/Vimar complex may be a promising drug target for diseases in which mitochondrial fission and fusion are dysfunctional
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