3 research outputs found
Phylogeographic Relationship of Honey Bee Dicistroviruses
No full textPhylogeographic Relationship of Honey Bee Dicistroviruse
Investigation of Changes in Expression Levels of Detoxification Genes and Mortality in Pieris brassicae Larvae Exposed to Nepeta meyeri
No full textThe plant extracts have been researched for the last thirty years to improve alternatives to chemical pesticides and to determine their insecticidal effects. It is also known that plant-based biopesticides are generally more specific and innocuous to other organisms. To date, it has been determined that many plant extracts have biocidal properties. However, research on the discovery of new plant extracts with better biocidal properties continues with great excitement. In this study, the biocidal effects of Nepeta meyeri on the larvae of the large cabbage white, Pieris brassicae (Lepidoptera: Pieridae), an important agricultural pest, were investigated. Three several doses (0.75, 1, and 1.25 mg/mL) of N. meyeri extract were given to the third instar larvae using the droplet feeding method. The larvae were taken to the living environment in the climate cabinet (26 & DEG;C, 65% humidity, 16 : 8 light : dark) and the mortality rates of the larvae were recorded. Mortality rates of larvae at 60 hours for 0.75, 1, and 1.25 mg/mL doses of the plant extract were 72, 77, and 92%, respectively. Also, expression changes in detoxification genes (cytochrome p450 and UDP-Glucuronosyltransferase) of randomly selected live larvae at 0, 12, 24, 36, 48, and 60 hours were detected by qRT-PCR. In this study, as a result of feeding the larvae with the highest dose of 1.25 mg/mL N. meyeri extract, the level of increase in the detoxification genes p450 and udp genes was at the highest level at the 12 hour. The results demonstrate that N. meyeri extract (1.25 mg/mL) was determined to be a promising botanical extract for the control of P. brassicae larvae
Genome sequence analysis of a Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) isolated from Heliothis peltigera in Turkey.
No full textThe entire genome of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) was sequenced, and compared to genomes of other existing isolates. HearNPV-TR genome is 130.691 base pairs with a 38.9% G+C content and has 137 open reading frames (ORFs) of ≥ 150 nucleotides. Five homologous repeated sequences (hrs) and two baculovirus repeated ORFs (bro-a and bro-b) were identified. Phylogenetic analysis showed that HearNPV-TR is closer to HaSNPV-C1, HaSNPV-G4, HaSNPV-AU and HasNPV. However, there are significant differences in hr3, hr5 regions and in bro-a gene. Pairwise Kimura-2 parameter analysis of 38 core genes sequences of HearNPV-TR and other Helicoverpa NPVs showed that the genetic distances for these sequences were below 0.015 substitutions/site. Genomic differences as revealed by restriction profiles indicated that hr3, hr5 regions and bro-a gene may play a role in the virulence of HearNPV-TR
