118,068 research outputs found
The crystal structure of a highly thermostable carboxyl esterase from Bacillus coagulans
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
The crystal structure of a new transaminase from the marine bacterium Virgibacillus
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
La taureau sacré de Chedenou
No full textGourlay Y. J. L. La taureau sacré de Chedenou. In: École pratique des hautes études, Section des sciences religieuses. Annuaire. Tome 86, 1977-1978. 1977. pp. 467-469
The crystal structure of Halomonas elongata amino-transferase
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
The crystal structure of secreted antigen BPSL2520
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
The structure of Trehalose-6-phosphatase from Burkholderia pseudomallei
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
The crystal structure of a highly thermostable carboxyl esterase from Bacillus coagulans in complex with glycerol
No full textIl file .pdb può essere aperto con i seguenti programmi: Pymol, coot, word o textedit
Crystal structure of an R-selective transaminase from Thermomyces stellatus
No full texthttps://www.wwpdb.org/pdb?id=pdb_00006xw
Widely applicable background depletion step enables transaminase evolution through solid-phase screening
No full textDirected evolution of transaminases is a widespread technique in the development of highly sought-after biocatalysts for industrial applications. This process, however, is challenged by the limited availability of effective high-throughput protocols to evaluate mutant libraries. Here we report a rapid, reliable, and widely applicable background depletion method for solid-phase screening of transaminase variants, which was successfully applied to a transaminase from Halomonas elongata (HEWT), evolved through rounds of random mutagenesis towards a series of diverse prochiral ketones. This approach enabled the identification of transaminase variants in viable cells with significantly improved activity towards parasubstituted acetophenones (up to 60-fold), as well as tetrahydrothiophen-3-one and related substrates. Rationalisation of the mutants was assisted by determination of the high-resolution wild-type HEWT crystal structure presented herein
- …
