67 research outputs found
Pseudo-Boolean Programming for Bivalent Optimization
No full textTitle: Pseudo-Boolean Programming for Bivalent Optimization, Author: M. Natesan, Location: ThodeThis thesis introduces an effective computational algorithm making
use of Boolean algebra for solving bivalent optimization problems with
linear and nonlinear constraints. This method is a combination of the
algorithm suggested by Hammer and the branch and bound method. The whole
system of constraints is replaced by a single Boolean resolvent function
and the solutions of this resolvent are found by branch and bound method
which are found to be the feasible solutions of the system of constraints.
Some practical applications are also discussed.ThesisMaster of Engineering (ME
A finite element superconvergence approximations for singularly perturbed system of convection-diffusion problems
No full textSpeeches of The Honourable Mr. G. K. Gokhale, CIE, Non-Official Member of the Viceroys Legislative Council with an Introduction and a Frontispiece
No full textQUORUM QUENCHING POTENTIALS OF PROBIOTIC ENTEROCOCCUS DURANS LAB38 AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
No full textObjective: he focus of this study was to explore the nuance strategy to combat the virulence factors of the pathogens by probiotic Enterococcus durans LAB38Methods: Probiotic attributes was determined by bile salt tolerance (0.5%) and Artemia gnotobiotic assay. Quorum sensing (QS) inhibitory activity of the supernatant and ethyl acetate (EA) extract of LAB38 was evaluated by using the indicator strains, includes Chromobacterium violaceum CV026 (mini-Tn5 mutant of ATCC 31532), methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PA). Reporter strains, Vibrio harveyi BB170 (luxN mutant), BB886 (luxP mutant) and Escherichia coli pSB401 (pACYC184- derived) were used for bioluminescence-based target specificity analysis. GC-MS analysis of EA extract was performed using standard protocol. Results: LAB38 has shown bile salt tolerance and positive probiotic effect towards Artemia salina. In addition, 100 µg/ml EA extract has significantly reduced the violacein production (37 ± 1.4%) in CV026, biofilm formation in MRSA (94 ± 0.9 %) and PA (22 ± 0.08%). Further, 200 µg/ml of EA extract has shown inhibition against both autoinducer-1 and autoinducer-2 mediated QS system. Bioluminescence inhibition is directly proportional to the time of exposure. GC-MS result revealed that bromine, sulphur containing molecule and azulene derivative were found in the EA extract.Conclusion This is the first report on probiotic Enterococcus durans for quorum quenching activity. Hence, the bacterium could be used for future therapeutics application. Keywords: Autoinducer, Biofilm, Methicillin resistant Staphylococcus aureus, Quorum quenching, Probiotics, CV026.</jats:p
Evaluation of growth performance of Penaeus monodon (Fabricius) fed diet with partial replacement of fishmeal by Spirulina platensis (Sp) meal
No full textA partial replacement of fish meal with Spirulina platensis (Sp) meal for the diet of juvenile shrimp Penaeus monodon (Fabricius) was analyzed for the growth and survival under laboratory condition. A total of five experimental diets were formulated with partial replacement of fishmeal by Sp meal, Sp-1 (14.3%), Sp-2 (28.6%), Sp-3 (42.8%), Sp-4 (57.14%), and the control diet (Sp meal free diet). The feeding trial was conducted for the period of 60 days. Juveniles fed with Sp meal supplemented diet showed significant (P less than 0.05) improvement of carcass composition (protein ratio and edible flesh). Higher growth and survival were obtained in P. monodon fed with Sp-3 diet, it was significantly (P less than 0.05) greater than the Sp meal free diet. The feeding efficiency (FE), specific growth rate (SGR) and survival (93.3%) of the shrimp was significantly (P less than 0.05) high in the Sp-3 diet. Survival rate was also higher in diet Sp-3 (42.8% substitution), but lower survival and growth rate observed in Sp-4 (57.14% substitution) and control diets. The Physico-chemical quality of the experimental tank water was better in Sp-3, and Sp-2 diet groups. This study recommended that Sp meal as alternative protein substitute of fish meal in shrimp diet.</jats:p
Probiotic effect of Lactobacillus acidophilus against vibriosis in juvenile shrimp (Penaeus monodon)
No full textThe application of effective probiotics in shrimp aquaculture is an excellent alternative for chemicals and antibiotics to prevent disease control. This study was carried out to evaluate the probiotic potential of Lactobacillus acidophilus 04 (home made curd isolate) on pathogenic Vibrio in shrimp Penaeus monodon juveniles. L. acidophilus showed antibacterial activity against Vibrio parahaemolyticus, Vibrio cholerae, Vibrio harveyi and Vibrio alginolyticus. The probiotic effect of L. acidophilus was tested by feeding juvenile shrimp (P. monodon) through feed (supplemented with 105 CFU g-1) for 30 days before and after an immersion challenge with V. alginolyticus at 105 CFU mL-1. Shrimp survival was determined after 10 days of challenge. The treatment with L. acidophilus 04 resulted in 20% final mortality as compared to 86.7% in the control group. Results of the study validated L. acidophilus 04 has potential probiotic principles to control pathogenic V. alginolyticus in shrimp aquaculture.Key words: Lactobacillus acidophilus, probiotic, shrimp, vibriosis
QUORUM QUENCHING POTENTIALS OF PROBIOTIC ENTEROCOCCUS DURANS LAB38 AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
No full textObjective: he focus of this study was to explore the nuance strategy to combat the virulence factors of the pathogens by probiotic Enterococcus durans LAB38Methods: Probiotic attributes was determined by bile salt tolerance (0.5%) and Artemia gnotobiotic assay. Quorum sensing (QS) inhibitory activity of the supernatant and ethyl acetate (EA) extract of LAB38 was evaluated by using the indicator strains, includes Chromobacterium violaceum CV026 (mini-Tn5 mutant of ATCC 31532), methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PA). Reporter strains, Vibrio harveyi BB170 (luxN mutant), BB886 (luxP mutant) and Escherichia coli pSB401 (pACYC184- derived) were used for bioluminescence-based target specificity analysis. GC-MS analysis of EA extract was performed using standard protocol. Results: LAB38 has shown bile salt tolerance and positive probiotic effect towards Artemia salina. In addition, 100 µg/ml EA extract has significantly reduced the violacein production (37 ± 1.4%) in CV026, biofilm formation in MRSA (94 ± 0.9 %) and PA (22 ± 0.08%). Further, 200 µg/ml of EA extract has shown inhibition against both autoinducer-1 and autoinducer-2 mediated QS system. Bioluminescence inhibition is directly proportional to the time of exposure. GC-MS result revealed that bromine, sulphur containing molecule and azulene derivative were found in the EA extract.Conclusion This is the first report on probiotic Enterococcus durans for quorum quenching activity. Hence, the bacterium could be used for future therapeutics application. Keywords: Autoinducer, Biofilm, Methicillin resistant Staphylococcus aureus, Quorum quenching, Probiotics, CV026
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