17 research outputs found
Moving beyond e-journals
No full textPaul Ayris explains to Elspeth Hyams why scholarly communication has moved beyond the debate on e-journals pricing and open access
Conference report: OAI7 — Raising the Bar for Current Developments in Scholarly Communication, A Personal View
No full textThe purpose of this paper is to give an overview of the presentations and sessions at the OAI7 Workshop in the University of Geneva, 22–24 June 2011. The theme of the Workshop was, as always, Innovations in Scholarly Communication. The Workshop was attended by a record number of 267 delegates. Thirty-four posters were submitted for the Poster session. There were 6 tutorials, 6 plenary sessions and 6 technical sessions. The paper identifies some of the highlights of the Workshop and updates two previous review articles by Professor Raf Dekeyser and the author on the history of the OAI meetings
Apigenin alleviates neuroinflammation in a mouse model of Parkinson’s disease
No full textPurpose of the study: The aim of this study is to evaluate the effect of apigenin on inflammatory response in brain tissue in Parkinson’s mouse model. Materials and methods: Parkinson’s disease model was induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Sixty 8-10-weeks-old male C57BL/6 mice were randomly divided into four groups control, Parkinson, prophylaxis, and treatment. Control (0.9% NaCl 0.5 ml, 10 days, i.p.), Parkinson (25 mg/kg MPTP, 5 days, i.p.), prophylaxis (50 mg/kg apigenin, 5 days + 25 mg/kg MPTP, 5 days, i.p.), and treatment (25 mg/kg MPTP, 5 days + 50 mg/kg apigenin, 5 days). The expressions and protein levels of tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), IL-6, IL-10, and transforming growth factor-beta (TGF-β) were determined using immunohistochemistry and enzyme-linked immunosorbent analysis. Results: Apigenin administration attenuated MPTP-induced histopathological changes in brain tissue. Furthermore, apigenin reversed the changes in expressions and concentrations of TNF-α, IL-1β, IL-6, IL-10, and TGF-β. Conclusion: This study suggests that apigenin could be used as a neuroprotective option to attenuate neuroinflammation in Parkinson’s disease
Epidermal growth factor concentration in milk of healthy water buffaloes (Bubalus bubalis)
No full textEpidermal growth factor (EGF) has biological roles, including embryonic organ development, breast morphogenesis, breast cell proliferation, and mammary development. This study aimed to measure EGF concentration and evaluate its relationship with somatic cell count (SCC) in healthy water buffaloes (Bubalus bubalis) milk. The study material was constituted of 120 milk samples obtained from 30 healthy water buffaloes between the ages of 3-6 years, negative for California mastitis test and SCC less than 3.00 × 105 cells mL-1 milk. In milk serum samples, the EGF concentration was measured using a bovine-specific enzyme-linked immunosorbent assay kit. Epidermal growth factor concentration in the buffalo milk was ranged from 4.30 to 9.80 ng mL-1, with a mean of 8.30 ± 1.50 ng mL-1. Positive correlation between milk SCC values and EGF concentrations was recorded in water buffaloes. Further research is required to evaluate the content of milk EGF in different species of animals because of the EGF effective role in mammary gland and intestinal mucosa
The milk IGF-2 level is positively correlated with milk yield in Anatolian water buffaloes
No full textThe aim of this study was to investigate the relationship between milk insulin-like growth factor-2 (IGF-2) concentration and milk yield in Anatolian water buffaloes. This study was conducted on milk samples from 80 Anatolian water buffaloes. Milk samples collected from buffaloes were evaluated for subclinical mastitis. For this purpose, the California mastitis test and somatic cell count were performed in milk samples taken from four different mammary lobes of buffaloes. The milk IGF-2 level was determined by an enzyme-linked immunosorbent assay kit. Milk IGF-2 concentration ranged from 21.6 ng/ml - 63.2 ng/ml in Anatolian water buffaloes. The IGF-2 concentration in Anatolian water buffaloes milk was 40.1±8.5 ng/ml. Milk IGF-2 was positively correlated with milk yield (r2=0.941, P<0.01). Our results showed that milk IGF-2 concentration was associated with milk yield in Anatolian buffaloes. These findings show that locally synthesized IGF-2 can affect milk yield. This study contributes to the understanding of the composition of buffalo milk, which has an important value in human nutrition. It is recommended to confirm the results of similar measurements in milk from other animal species used for human consumption
The Effect of Seasons on Total Antioxidant Capacity (TAC), Total Oxidant Capacity (TOC) and OSI (Oxidative Stress Index) Levels in Free-Living Blackbirds (Turdus merula) in Nature
No full textNobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model
No full textThe aim of this study is to investigate the potential preventive and therapeutic effects of nobiletin by evaluating the expression of cytokines associated with inflammatory reactions in an autoimmune encephalomyelitis mouse model. A total of 60 male C57BL/6 mice aged between 8 and 10 weeks were used. Mice were divided into six groups (n = 10 mice per group): control, EAE, low-prophylaxis, high-prophylaxis, low-treatment and hightreatment. Experimental autoimmune encephalomyelitis (EAE) was induced by myelin oligodendrocyte glycoprotein (MOG) and pertussis toxin. Nobiletin was administered in low (25 mg/kg) and high (50 mg/kg) doses, intraperitoneally. The prophylactic and therapeutic effects of nobiletin on brain tissue and spinal cord were evaluated by expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN gamma), IL-6, IL-10 and transforming growth factor-beta (TGF-beta) using immunohistochemistry and real-time polymerase chain reaction (RT-PCR). Prophylactic and therapeutic use of nobiletin inhibited EAE-induced increase of TNF-alpha, IL-1 beta and IL-6 activities to alleviate inflammatory response in brain and spinal cord. Moreover, nobiletin supplement dramatically increased the IL-10, TGF-beta and IFN gamma expressions in prophylaxis and treatment groups compared with the EAE group in the brain and spinal cord. The results obtained from this study show that prophylactic and therapeutic nobiletin modulates expressions of proinflammatory and antiinflammatory cytokines in brain and spinal cord dose-dependent manner in EAE model. These data demonstrates that nobiletin has a potential to attenuate inflammation in EAE mouse model. These experimental findings need to be supported by clinical studies
Effects of amitraz plus Parapoxvirus ovis on EGF, VEGF, IGF-1 and IGF-2 in canine generalized demodicosis
No full textObjective. The purpose of the study is to investigate the effect of treatment with amitraz plus-Parapoxvirus ovis (IPPVO) on serum concentrations and skin expressions of insulin-like growth factor (IGF)-1 and -2, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), in dogs suffering from generalised demodicosis (GD). Materials and Methods. GD affected dogs were injected 1 ml IPPVO on days 0, 2 and 9 subcutaneously in addition to amitraz (0.025%) treatment twice weekly for 80 days. IGF-1, IGF-2, EGF and VEGF concentrations in blood serum were measured by canine-specific enzyme-linked immunosorbent assay kit. EGF, VEGF, IGF-1, and IGF-2 expressions in skin biopsy specimens were examined immunohistochemically. Results. After the treatment of the dogs with amitraz plus-IPPVO in GD, we demonstrated a significant reduction in both circulating concentrations and skin expressions of EGF, VEGF, IGF-1, and IGF-2, which have a role in preserving skin integrity and wound healing. Conclusions. Results of this study suggest that IGF-1, IGF-2 EGF, and VEGF have a crucial role in the progression of GD in dogs. It is believed that the findings from this study will contribute to the development of new strategies for the treatment of GD, which is an important health problem for dogs. © The Author(s) 2021. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/), lets others remix, tweak, and build upon your work non-commercially, as long as they credit you and license their new creations under the identical terms.Ondokuz Mayis Üniversites
Efectos de amitraz más Parapoxvirus ovis sobre EGF, VEGF, IGF-1 e IGF-2 en la demodicosis generalizada canina
No full textObjective. The purpose of the study is to investigate the effect of treatment with amitraz plus-Parapoxvirus ovis (IPPVO) on serum concentrations and skin expressions of insulin-like growth factor (IGF)-1 and -2, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), in dogs suffering from generalised demodicosis (GD). Materials and Methods. Generalised demodicosis affected dogs were injected 1 ml IPPVO on days 0, 2 and 9 subcutaneously in addition to amitraz (0.025 %) treatment twice weekly for 80 days. IGF-1, IGF-2, EGF and VEGF concentrations in blood serum were measured by canine-specific enzyme-linked immunosorbent assay kit. EGF, VEGF, IGF-1, and IGF-2 expressions in skin biopsy specimens were examined immunohistochemically. Results. After the treatment of the dogs with amitraz plus-IPPVO in GD, we demonstrated a significant reduction in both circulating concentrations and skin expressions of EGF, VEGF, IGF-1, and IGF-2, which have a role in preserving skin integrity and wound healing. Conclusions. Results of this study suggest that IGF-1, IGF-2 EGF, and VEGF have a crucial role in the progression of GD in dogs. It is believed that the findings from this study will contribute to the development of new strategies for the treatment of GD, which is an important health problem for dogs.Objetivo. El propósito del estudio es investigar el efecto del tratamiento con amitraz más-Parapoxvirus ovis (IPPVO) sobre las concentraciones séricas y las expresiones cutáneas del factor de crecimiento insulínico (IGF) -1 y -2, factor de crecimiento epidérmico (EGF), vascular factor de crecimiento endotelial (VEGF), en perros que padecen demodicosis generalizada (GD). Materiales y métodos. A los perros afectados por GD se les inyectó 1 mL de IPPVO los días 0, 2 y 9 por vía subcutánea además del tratamiento con amitraz (0,025%) dos veces por semana durante 80 días. Las concentraciones de IGF-1, IGF-2, EGF y VEGF en suero sanguíneo se midieron mediante un kit de ensayo inmunoabsorbente ligado a enzimas específico para caninos. Las expresiones de EGF, VEGF, IGF-1 e IGF-2 en muestras de biopsia de piel se examinaron inmunohistoquímicamente. Resultados. Después del tratamiento de los perros con amitraz más-IPPVO en GD, demostramos una reducción significativa tanto en las concentraciones circulantes como en las expresiones cutáneas de EGF, VEGF, IGF-1 e IGF-2, que tienen un papel en la preservación de la integridad de la piel y la herida. curación. Conclusiones. Los resultados de este estudio sugieren que IGF-1, IGF-2 EGF y VEGF tienen un papel crucial en la progresión de la GD en perros. Se cree que los hallazgos de este estudio contribuirán al desarrollo de nuevas estrategias para el tratamiento de la GD, que es un problema de salud importante para los perros
Efectos de amitraz más Parapoxvirus ovis sobre EGF, VEGF, IGF-1 e IGF-2 en la demodicosis generalizada canina
No full textObjective. The purpose of the study is to investigate the effect of treatment with amitraz plus-Parapoxvirus ovis (IPPVO) on serum concentrations and skin expressions of insulin-like growth factor (IGF)-1 and -2, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), in dogs suffering from generalised demodicosis (GD). Materials and Methods. Generalised demodicosis affected dogs were injected 1 ml IPPVO on days 0, 2 and 9 subcutaneously in addition to amitraz (0.025 %) treatment twice weekly for 80 days. IGF-1, IGF-2, EGF and VEGF concentrations in blood serum were measured by canine-specific enzyme-linked immunosorbent assay kit. EGF, VEGF, IGF-1, and IGF-2 expressions in skin biopsy specimens were examined immunohistochemically. Results. After the treatment of the dogs with amitraz plus-IPPVO in GD, we demonstrated a significant reduction in both circulating concentrations and skin expressions of EGF, VEGF, IGF-1, and IGF-2, which have a role in preserving skin integrity and wound healing. Conclusions. Results of this study suggest that IGF-1, IGF-2 EGF, and VEGF have a crucial role in the progression of GD in dogs. It is believed that the findings from this study will contribute to the development of new strategies for the treatment of GD, which is an important health problem for dogs.Objetivo. El propósito del estudio es investigar el efecto del tratamiento con amitraz más-Parapoxvirus ovis (IPPVO) sobre las concentraciones séricas y las expresiones cutáneas del factor de crecimiento insulínico (IGF) -1 y -2, factor de crecimiento epidérmico (EGF), vascular factor de crecimiento endotelial (VEGF), en perros que padecen demodicosis generalizada (GD). Materiales y métodos. A los perros afectados por GD se les inyectó 1 mL de IPPVO los días 0, 2 y 9 por vía subcutánea además del tratamiento con amitraz (0,025%) dos veces por semana durante 80 días. Las concentraciones de IGF-1, IGF-2, EGF y VEGF en suero sanguíneo se midieron mediante un kit de ensayo inmunoabsorbente ligado a enzimas específico para caninos. Las expresiones de EGF, VEGF, IGF-1 e IGF-2 en muestras de biopsia de piel se examinaron inmunohistoquímicamente. Resultados. Después del tratamiento de los perros con amitraz más-IPPVO en GD, demostramos una reducción significativa tanto en las concentraciones circulantes como en las expresiones cutáneas de EGF, VEGF, IGF-1 e IGF-2, que tienen un papel en la preservación de la integridad de la piel y la herida. curación. Conclusiones. Los resultados de este estudio sugieren que IGF-1, IGF-2 EGF y VEGF tienen un papel crucial en la progresión de la GD en perros. Se cree que los hallazgos de este estudio contribuirán al desarrollo de nuevas estrategias para el tratamiento de la GD, que es un problema de salud importante para los perros
