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    PRELIMINARY STUDY ON FUMARIC ACID PRODUCTION FROM GRAPE MUST BY CANDIDA HYDROCARBOFUMARICA

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    The production of fumaric acid from grape must by Candida hydrocarbofumarica ATCC 28532 was studied in shake-flask by varying the initial concentrations of nitrogen and reducing sugars in accordance with a statistical experimental design. A fumaric acid production of about 7.5 g/l was achieved after a residence time of 7 days in presence of the initial nitrogen level lower than 1.10 g/l and of the glucose content varied from 51 to 115 g/l. At higher glucose concentrations (140 g/l) and C/N ratio constant to 150, after 14 days the microbial process yielded 13.15 g/l of fumaric acid with a productivity of 0.94 g/l per day and a total organic acid yeld of 28.3%
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