21 research outputs found
Sets with more differences than sums
No full textWe show that a random set of integers with density 0 has almost always more differences than sums
d-GDM: A mobile diagnostic decision support system for gestational diabetes
No full textABSTRACT Objective The aim of the study is to describe a portable and convenient software to facilitate the diagnostics of gestational (GDM) and pre-gestational diabetes (PGDM). Materials and methods An open source software, d-GDM, was developed in Java. The integrated development environment Android Studio was used as the Android operational system. The software for GDM diagnosis uses the criteria endorsed by the International Association of Diabetes and Pregnancy Study Group, modified by the World Health Organization. Results GDM diagnosis criteria is not simple to follow, therefore, errors or inconsistencies in diagnosis are expected and could delay the appropriate treatment. The d-GDM, was developed to assist GDM diagnosis with precision and consistency diagnostic reports. The open source software can be manipulated conveniently. The operator requires information regarding the gestational period and selects the appropriate glycaemic marker options from the menu. During operation, pressing the button “diagnosticar” on the screen will present the diagnosis and information for the follow up. d-GDM is available in Portuguese or English and can be downloaded from the Google PlayStore. A responsive web version of d-GDM is also available. The usefulness and accuracy of d-GDM was verify by field tests involving 22 subjects and 5 mobile phone brands. The approval regards user-friendliness and efficiency were 95% or higher. The GDM diagnosis were 100% correct, in this pilot test. d-GDM is a user-friendly, free software for diagnosis that was developed for mobile devices. It has the potential to contribute and facilitate the diagnosis of gestational diabetes for healthcare professionals.</div
Comparative Proteomics Analysis of the Rice Roots Colonized by <i>Herbaspirillum seropedicae</i> Strain SmR1 Reveals Induction of the Methionine Recycling in the Plant Host
No full textAlthough the use of plant growth-promoting
bacteria in agriculture is a reality, the molecular basis of plant–bacterial
interaction is still poorly understood. We used a proteomic approach
to study the mechanisms of interaction of Herbaspirillum seropedicae SmR1 with rice. Root proteins of rice seedlings inoculated or noninoculated
with H. seropedicae were separated by 2-D electrophoresis.
Differentially expressed proteins were identified by MALDI-TOF/TOF
and MASCOT program. Among the identified proteins of H. seropedicae, the dinitrogenase reductase
NifH and glutamine synthetase GlnA, which participate in nitrogen
fixation and ammonium assimilation, respectively, were the most abundant.
The rice proteins up-regulated included the S-adenosylmethionine
synthetase, methylthioribose kinase, and acireductone
dioxygenase 1, all of which are involved in the methionine recycling. S-Adenosylmethionine synthetase catalyzes the synthesis
of S-adenosylmethionine, an intermediate used in
transmethylation reactions and in ethylene, polyamine, and phytosiderophore
biosynthesis. RT-qPCR analysis also confirmed that the methionine
recycling and phytosiderophore biosynthesis genes were up-regulated,
while ACC oxidase mRNA level was down-regulated in rice roots colonized
by bacteria. In agreement with these results, ethylene production
was reduced approximately three-fold in rice roots colonized by H. seropedicae. The results suggest that H. seropedicae stimulates methionine recycling and phytosiderophore
synthesis and diminishes ethylene synthesis in rice roots
Investigation of Chalcones as Selective Inhibitors of the Breast Cancer Resistance Protein: Critical Role of Methoxylation in both Inhibition Potency and Cytotoxicity
No full textABCG2 plays a major role in anticancer-drug efflux and
related
tumor multidrug resistance. Potent and selective ABCG2 inhibitors
with low cytotoxicity were investigated among a series of 44 chalcones
and analogues (1,3-diarylpropenones), by evaluating their inhibitory
effect on the transport of mitoxantrone, a known ABCG2 substrate.
Six compounds producing complete inhibition with IC50 values
below 0.5 μM and high selectivity for ABCG2 were identified.
The number and position of methoxy substituents appeared to be critical
for both inhibition and cytotoxicity. The best compounds, with potent
inhibition and low toxicity, contained an N-methyl-1-indolyl
(compound 38) or a 6′-hydroxyl-2′,4′-dimethoxy-1-phenyl
(compound 27) moiety (A-ring) and two methoxy groups
at positions 2 and 6 of the 3-phenyl moiety (B-ring). Methoxy substitution
contributed to inhibition at positions 3 and 5, but had a negative
effect at position 4. Finally, methoxy groups at positions 3, 4, and
5 of the B-ring markedly increased cytotoxicity and, therefore, should
be avoided
Substituted Chromones as Highly Potent Nontoxic Inhibitors, Specific for the Breast Cancer Resistance Protein
No full textA series of 13 disubstituted chromones was synthesized.
Two types
of substituents, on each side of the scaffold, contributed to both
the potency of ABCG2 inhibition and the cytotoxicity. The best compound,
5-(4-bromobenzyloxy)-2-(2-(5-methoxyindolyl)ethyl-1-carbonyl)-4H-chromen-4-one (6g), displayed high-affinity
inhibition and low cytotoxicity, giving a markedly high therapeutic
index. The chromone derivative specifically inhibited ABCG2 versus
other multidrug ABC transporters and was not transported. It constitutes
a highly promising candidate for in vivo chemosensitization
of ABCG2-expressing tumors
Gaussian Job Archive for C15H25NO3
No full textArchive of work produced using the resources of the Imperial College High Performance Computing Service
Cytotoxic effects of 1,3,4-thiadiazolium derivatives on hepatocytes.
No full text<p>A. MTT assay (the experimental conditions are described in the Materials and Methods section 2.5.1) The cells were seeded with or without 1,3,4-thiadiazolium derivatives at 25 for 18–24 h. The results were expressed as % of viability in comparison to control. B. LDH release assay (the experimental conditions are described in the Materials and Methods section 2.5.2). Under the same treatment conditions described above, LDH activity was measured in the supernatants. Data represent means of four different experiments in quadruplicate. The results were expressed as % of viability in comparison to control. ** and *** denotes values significantly different from the control or between the different treatments at <i>P</i>< 0.01 and <i>P</i>< 0.0001, respectively.</p
Data_Sheet_1.DOCX
No full textThe ability of bacteria to produce polyhydroxyalkanoates such as poly(3-hydroxybutyrate) (PHB) enables provision of a carbon storage molecule that can be mobilized under demanding physiological conditions. However, the precise function of PHB in cellular metabolism has not been clearly defined. In order to determine the impact of PHB production on global physiology, we have characterized the properties of a ΔphaC1 mutant strain of the diazotrophic bacterium Herbaspirillum seropedicae. The absence of PHB in the mutant strain not only perturbs redox balance and increases oxidative stress, but also influences the activity of the redox-sensing Fnr transcription regulators, resulting in significant changes in expression of the cytochrome c-branch of the electron transport chain. The synthesis of PHB is itself dependent on the Fnr1 and Fnr3 proteins resulting in a cyclic dependency that couples synthesis of PHB with redox regulation. Transcriptional profiling of the ΔphaC1 mutant reveals that the loss of PHB synthesis affects the expression of many genes, including approximately 30% of the Fnr regulon.</p
Table_2.XLSX
No full textThe ability of bacteria to produce polyhydroxyalkanoates such as poly(3-hydroxybutyrate) (PHB) enables provision of a carbon storage molecule that can be mobilized under demanding physiological conditions. However, the precise function of PHB in cellular metabolism has not been clearly defined. In order to determine the impact of PHB production on global physiology, we have characterized the properties of a ΔphaC1 mutant strain of the diazotrophic bacterium Herbaspirillum seropedicae. The absence of PHB in the mutant strain not only perturbs redox balance and increases oxidative stress, but also influences the activity of the redox-sensing Fnr transcription regulators, resulting in significant changes in expression of the cytochrome c-branch of the electron transport chain. The synthesis of PHB is itself dependent on the Fnr1 and Fnr3 proteins resulting in a cyclic dependency that couples synthesis of PHB with redox regulation. Transcriptional profiling of the ΔphaC1 mutant reveals that the loss of PHB synthesis affects the expression of many genes, including approximately 30% of the Fnr regulon.</p
Table_1.XLSX
No full textThe ability of bacteria to produce polyhydroxyalkanoates such as poly(3-hydroxybutyrate) (PHB) enables provision of a carbon storage molecule that can be mobilized under demanding physiological conditions. However, the precise function of PHB in cellular metabolism has not been clearly defined. In order to determine the impact of PHB production on global physiology, we have characterized the properties of a ΔphaC1 mutant strain of the diazotrophic bacterium Herbaspirillum seropedicae. The absence of PHB in the mutant strain not only perturbs redox balance and increases oxidative stress, but also influences the activity of the redox-sensing Fnr transcription regulators, resulting in significant changes in expression of the cytochrome c-branch of the electron transport chain. The synthesis of PHB is itself dependent on the Fnr1 and Fnr3 proteins resulting in a cyclic dependency that couples synthesis of PHB with redox regulation. Transcriptional profiling of the ΔphaC1 mutant reveals that the loss of PHB synthesis affects the expression of many genes, including approximately 30% of the Fnr regulon.</p
