77 research outputs found
Encapsulation of eukaryotic cells in alginate microparticles: cell signaling by TNF-alpha through capsular structure of cystic fibrosis cells
No full textEntrapment of mammalian cells in natural or synthetic biomaterials represents an important tool for both basic and applied research in tissue engineering. For instance, the encapsulation procedures allow to physically isolate cells from the surrounding environment, after their transplantation maintaining the normal cellular physiology. The first part of the current paper describes different microencapsulation techniques including bulk emulsion technique, vibrating-nozzle procedure, gas driven mono-jet device protocol and microfluidic based approach. In the second part, the application of a microencapsulation procedure to the embedding of IB3-1 cells is also described. IB3-1 is a bronchial epithelial cell line, derived from a cystic fibrosis (CF) patient. Different experimental parameters of the encapsulation process were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of protein secretion, analysing the culture medium by Bio-Plex strategy. The analyzed factors include members of the interleukin family (IL-6), chemokines (IL-8 and MCP-1) and growth factors (G-CSF). The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent
Induction by TNF-a of IL-6 and IL-8 in cystic fibrosis bronchial IB3-1 epithelial cells encapsulated in alginate microbeads.
No full textAbstract: We have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. The applied method is based on generation of monodisperse droplets by a vibrational nozzle. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of secretomic profile, analyzing the culture medium by Bio-Plex strategy. The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells could be induced to pro-inflammatory responses, after treatment with TNF-a. In this experimental set-up, encapsulated and free IB3-1 cells were treated with TNF-a, thereafter the culture media from both cell populations were collected. As expected, TNF-a induced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of interleukin-6 and interleukin-8 occurs also by encapsulated IB3-1 cells
TNF-alpha induced release of pro-inflammatory proteins by cystic fibrosis IB3-1 cells encapsulated in alginate microbeads
No full textThe inflammatory processes activated in cystic fibrosis are complex and need the development of specific systems to possibly study the mechanism of bacterial activation of cystic fibrosis cells, as well as the effect of the secreted chemokines on target cell populations. To this aim, we have developed an ionic alginate microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. This microencapsulation procedure does not alter viability and the secretomic profile of encapsulated IB3-1 cells. Most of the analyzed proteins (members of the interleukin family, chemokines, growth factors and soluble forms of adhesion molecules), using Bio-plex technology, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, encapsulated and free IB3-1 cells were treated with TNF-alpha and after 24 hours the culture media from both cell populations were collected. As expected, TNF-alpha induced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of IL-6 and IL-8 occurs also by encapsulated IB3-1 cells. In conclusion, the encapsulation of secreting cells in alginate microbeads represents a promising strategy for biotechnology applications in tissue engineering and biomedicine
TNF-alpha induced release of IL-8 by IB3-1 cells encapsulated in alginate microbeads
No full textWe have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. The applied method is based on the generation of monodisperse droplets by a vibrational nozzle; for the microbeads hardening, an ionic alginate encapsulation procedure was utilized. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. The produced barium-alginate microbeads posses excellent properties, with respect to morphological parameters as well as narrow size distribution. Results, reproducibly obtained, firmly demonstrate that the microencapsulation procedure do not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of secretomic profile, analysing the culture medium by Bio-Plex strategy. The analyzed factors include members of the interleukin family, chemokines, growth factors and soluble forms of adhesion molecules. The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells, could be induced to pro-inflammatory responses, after treatment with TNF-alpha. In this experimental set-up, encapsulated and free IB3-1 cells were treated, for 24 hours, with TNF-alpha, thereafter the culture media from both cell populations were collected. As expected, TNF-alpha induced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of IL-6 and IL-8 occurs also by encapsulated IB3-1 cells. In conclusion, the described encapsulation procedure represents a promising strategy to utilize encapsulation of secreting cells in alginate microbeads, as a very interesting approach for biotechnology applications in tissue engineering and biomedicine
Human bladder carcinoma cell line 5637 encapsulated in alginate microbeads: release of interleukins, chemokines and growth factors.
No full textWe have developed a microencapsulation procedure for the entrapment and manipulation of the human bladder carcinoma cell line 5637. This cell line is a well-known source of hematopoietic cytokines. For istance 5637 cell line-conditioned medium (5637 CM) is used for the culture of growth factor–dependent hematopoietic primary cells and cell lines. The applied method is based on the generation of monodisperse droplets by a vibrational nozzle; for the microbeads hardening, an ionic alginate encapsulation procedure was utilized. Our results demonstrate that the microencapsulation procedure do not alter the viability of the encapsulated 5637 cells. Moreover, the encapsulated 5637 cells have been characterized for secretomic profile using Bio-Plex system. The obtained results demonstrated that most of the analyzed proteins were secreted by both the free and encapsulated cells with an almost superimposable pattern. In both cases, the most released proteins were G-CSF, VEGF, IL-8, GM-CSF, IP-10, IL-6, PDGF-BB, IL1ra, INF-gamma Finally, we determined whether 5637 CM obtained from encapsulated 5637 cells sustain the proliferation of primary erythroid cell cultures isolated from normal donors. The results demonstrate this 5637 CM stimulates the proliferation of erythroid precursor cells and maintain their capacity of erytho-differentiate and produce Hb. In conclusion, the described encapsulation procedure represents a promising strategy to utilize encapsulation of secreting cells in alginate microbeads, as a very interesting approach for biotechnology applications in tissue engineering and biomedicine
COVID-19 and myotonic dystrophy: Case reports and systematic review
No full textIntroduction: Steinert's disease is a rare genetic disorder characterized by progressive myotonia and multi-organ damage. It is associated with respiratory and cardiological complications often leading patients to exitus. These conditions are also traditional risk factors for severe COVID-19. SARS-CoV-2 has affected people with chronic diseases, but the impact on people with Steinert's disease is poorly defined, with only a few reported and described. More data are needed to understand whether this genetic disease is a risk factor for more serious evolution or death in patients with COVID-19.Methodology: The study describes two cases of patients with SD and COVID-19 and summarizes available evidence of the clinical outcome of COVID-19 in patients with Steinert's disease, by performing a systematic review of the literature (following PRISMA statements and performing PROSPERO registration).Results: Overall, 5 cases were retrieved from the literature review, with a median age of 47 years, of whom 4 had advanced SD and unfortunately died. By contrast, the 2 patients from our clinical practice and 1 from literature had a good clinical outcomes. Mortality ranged from 57% (all cases) to 80% (only literature review).Conclusions: There is a high mortality rate in patients with both Steinert's disease and COVID-19. It highlights the importance of strengthening prevention strategies, especially vaccination. All SD with SARS-CoV-2 infection/COVID-19 patients should be identified early and treated to avoid complications. It is still unknown which treatment regimen is best to use in those patients. Studies on a greater number of patients are necessary to provide clinicians with further evidence
Limb Salvage and Survival in Chronic Limb-Threatening Ischemia: The Need for a Fast-Track Team-Based Approach
No full textChronic limb-threatening ischemia (CLTI) represents the end-stage form of peripheral arterial disease (PAD) and is associated with a very poor prognosis and high risk of limb loss and mortality. It can be considered very similar to a terminal cancer disease, reflecting a large impact on quality of life and healthcare costs. The aim of this study is to offer an overview of the relationship between CLTI, limb salvage, and mortality, with a focus on the need of a fast-track team-based management that is a driver to achieve better survival results. This review can be useful to improve management of this growing impact disease, and to promote the standardisation of care and communication between specialist and non-specialist healthcare professionals
The risk for type B aortic dissection in Marfan syndrome
No full textMarfan syndrome is the most prevalent connective tissue disorder, with an autosomal dominant inheritance with variable penetrance. This paper aims to summarize epidemiology and treatment for type B dissection in Marfan patients
A Lagrangian sub-grid model for the dispersion of clouds of tracers
No full textTurbulence models are expected to satisfy the conflicting requirements of accuracy and computational efficiency. Here we discuss a new model that was recently developed in order to accurately and efficiently describe the dynamic of a clouds of tracers particles in Large Eddy Simulations of homogeneous and isotropic turbulent flows. The models incorporates the multi-scale nature of time and space turbulent velocity correlations that are essential in order to correctly reproduce the relative dispersion of multi-particle clouds. The model can be seen as an off-grid solver for the Eulerian velocity field at the positions of a given number of Lagrangian tracers that self-consistently move with it. Extensions to non homogeneous and isotropic turbulence as well as to the dynamics of particles will be discussed
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