1,720,997 research outputs found
Detection of Cucumber Mosaic Virus in Peanut ( Arachis hypogaea L.) in Argentina
Full text linkPeanut or groundnut (Arachis hypogaea L.) is an important oilseed crop in Argentina. Virus-like symptoms were observed in this crop during the 2000 growing season. Infected plants contained isometric particles c. 30 nm in diameter and tissue extracts reacted with a polyclonal antiserum to cucumber mosaic virus (CMV). A restriction profile typical of subgroup II was obtained for MspI cleavage of cDNA produced by using specific primers to CMV capsid protein (CP). These results were also confirmed by nucleotide sequence data on the CP gene. This is the first report of CMV subgroup II infecting peanut.Fil: de Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Lenardon, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentin
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
Full text linkA full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: de Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentin
Filodinámica del virus de la mancha anillada de la papaya en carica papaya L en Cuba
Full text linkPapaya is broadly cultivated in tropical and subtropical regions. However, they are affected by the Papaya ringspot virus (PRSV), which is the most economically important virus affecting papaya worldwide. This study aimed to determine the phylodynamics of PRSV on papaya in Cuba. For this purpose, orchardand garden papaya crops grown in 47 Cuban municipalities were surveyed from 2008 to 2013, revealing thewidespread distribution of PRSV in Cuba. Phylodynamic analyses performed with the coat protein partial geneof all Cuban PRSV-P isolates (34 sequences) and 107 sequences of isolates from the American continent and theCaribbean islands showed a most recent common ancestor in 1942 (HPD95 % = 1911- 1967). The substitutionrate was estimated to be 7.7 × 10-4 substitutions per site per year (HPD95 % = 4.6 × 10-4-1.1 × 10-3), whichis equivalent to those detected in other RNA viruses. Demographic reconstruction of PRSV showed that viraldiversity increased in the 1985-1990 period, which coincides with the implementation of extensive productionpractices. Moreover, in Cuba viral dispersion introductions were observed to occurre from Mexico and otherunknown ancestral localization. The spatio-temporal diffusion analysis proposed Mexico as an ancestral area forthe origin of diversification in the American continent and suggests new dispersion events between Americanand Caribbean isolates. The observed widespread distribution, clear geographic grouping of Cuban isolates, virusgrowth and genetic diversity provide strong evidence of the PRSV dispersion patterns.Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Torres, Carolina Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Portal, Orelvis. Universidad Central Marta Abreu de Las Villas; CubaTercer Seminario Internacional de Sanidad AgropecuariaVaraderoCubaCentro Nacional de Sanidad Agropecuari
Biological and molecular characterization of an isolate of Pelargonium zonate spot virus infecting sunflower in Argentina
Full text linkSunflower (Helianthus annuus) plants showing chlorotic concentric rings and line patterns on the leaves were observed in field crops near Paraná city (Entre Ríos, Argentina). Virus-enriched preparations examined with a trasmission electron microscope contained quasi-spherical particles ca. 33 nm in diameter. Symptomatic sunflower samples were serologically negative for six known members of the family Bromoviridae when tested with commercial antisera, but in later tests gave a positive reaction with a Pelargonium zonate spot virus (PZSV) antiserum. A virus was mechanically transmitted to 16 plant species belonging to four families. Its complete genomic sequence, obtained by pyrosequencing, had an organization typical of members of the family Bromoviridae. Three contigs resulting from de novo assembly of deep sequencing reads showed 90.0%, 94.7% and 93.9% nucleotide identity with RNA-1 (GenBank accession No. AJ272327), RNA-2 (AJ272328) and RNA-3 (AJ272329), respectively, of Pelargonium zonate spot virus (PZSV), a member of the genus Anulavirus described in Italy. Therefore, the virus associated with chlorotic concentric rings and line pattern symptoms in sunflower was identified as an isolate of PZSV. To our knowledge, this is the first reference to PZSV infecting sunflower worldwide and the first report of its presence in South America.Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Visintin, G. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: de Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentin
Molecular characterization of Sunflower chlorotic mottle virus: A member of a distinct species in the genus Potyvirus
Full text linkThe complete nucleotide (nt) and deduced amino acid (aa) sequences of the C (common) and CRS (chlorotic ringspot) Argentine strains of SuCMoV have been determined. The SuCMoV-C RNA genome consists of 9,965 nt, whereas indels within the P1 coding region of SuCMoV-CRS make its genomic length 15 nt shorter. Nucleotide and aa sequence identities between the polyproteins of the C and CRS strains of SuCMoV were 92.3 and 95.6%, respectively. Pairwise comparisons between the polyproteins of the C and CRS strains of SuCMoV and the viruses of the Potato virus Y (PVY) subgroup revealed identities of 66.5-66.9% at the nt level and 69.7-69. 8% at the aa level. These results and phylogenetic analyses show that although SuCMoV strains cluster together with the potyviruses belonging to the PVY subgroup, SuCMoV should be considered a member of a distinct species in the genus Potyvirus. © 2010 Springer-Verlag.Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: de Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentin
Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
No full textLuffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Central Marta Abreu de Las Villas; Cuba. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Portal Villafaña, Orelvis. Universidad Central Marta Abreu de Las Villas; Cub
Cucumber mosaic virus infecting ‘Cavendish’ banana in Argentina
Full text linkBanana plants (Musa sp.) showing severe mosaic symptoms in leaves were observed in Laguna Naineck, Formosa, Argentina. Electron microscopy observations of leaf dip preparations revealed isometric particles ca. 30 nm in diameter, which were identified as cucumber mosaic virus (CMV) by enzyme-linked immunosorbent assay (ELISA) test and reverse transcription-polymerase chain reaction (RT-PCR). CMV was mechanically transmitted to healthy Nicotiana glutinosa plants. Sequence analysis of coat protein gene showed highest nucleotides identity with other CMV isolates from United States of America, Serbia, Russia, Democratic Republic of Congo, Iran and Brazil, ranging between 96.9–98.3%. Phylogenetic analysis indicated that the virus isolate named Laguna Naineck (MH716245) belongs to the CMV group IA. To our knowledge, this is the first report of CMV in banana in Argentina. Moreover, constitutes the first Argentine CMV isolate available in the international nucleotide sequence databases.Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jaramillo Zapata, Margarita. Universidad San Pablo-T; ArgentinaFil: Villarreal Filipovich, José. Centro de Validación de Tecnologías Agropecuarias; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Torres, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Portal, Orelvis. Universidad Central Marta Abreu de Las Villas; CubaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin
Genome characterization of an Argentinean isolate of alfalfa leaf curl virus
Full text linkWe investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: de Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodríguez-Pardina, Patricia Elsa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Lenardon, Sergio Luis. Universidad Nacional de Río Cuarto; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin
Effects of Cucumber mosaic virus on yield and yield components of peanut
No full textIn Argentina, peanut (Arachis hypogaea) is naturally infected by Cucumber mosaic virus (CMV), subgroup II. The study involved the evaluation of the effects on yield and yield components in response to CMV infection at different growth stages: 4-6 developed nodes on the main axis (V4-6), 12-16 developed nodes on the main axis (V12-16) and onset of bloom (R1). The results showed that CMV infection significantly reduces peanut seed yield, mainly due to a severe decrease in seed average weight which declined approximately by 30%, 20% and 11% when the virus was inoculated at V4-6, V12-16 and R1, respectively. The number of pods, seeds, and seeds per pod were affected only when the virus was inoculated at V4-6. The percentage of confectionery peanut was also significantly affected when CMV was inoculated at vegetative crop stages. In addition, seed size and weight/volume ratio were greatly affected by the virus since the infected plants produced a higher proportion of small seeds. Larger seeds decreased when the plants were infected at earlier growth stages. Finally, infected plants produced a significantly greater number of immature pods than the healthy ones.Fil: de Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; ArgentinaFil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentin
Development and validation of PCR assays for detection of alfalfa dwarf disease-associated viruses in Australian lucerne pastures
Full text linkIn 2010, a severe disease of lucerne (Medicago sativa L.) named alfalfa dwarf disease (ADD) was reported in Argentina. High throughput sequencing of diseased plants indicated the presence of five viruses, the (−) ssRNA virus, alfalfa dwarf virus (ADV), the (+) RNA viruses, alfalfa mosaic virus (AMV), bean leafroll virus (BLRV) and alfalfa enamovirus 1 (AEV-1) and the ssDNA virus, alfalfa leaf curl virus (ALCV). In this study, we determined which ADD-associated viruses are present in Australian lucerne that showed diverse virus-like symptoms. A duplex RT-PCR was developed for simultaneous detection of ADV and AMV using a cloned non-infectious ADV RNA fragment as positive control. Similarly, the presence of BLRV and AEV-1 was determined by duplex RT-PCR, and ALCV by PCR. Only AMV and BLRV that are endemic to Australia were detected. None of the novel exotic viruses ADV, AEV-1 and ALCV were detected in lucerne samples collected between 2015 and 2017. However, AMV and BLRV were detected in 78% and 70% of tested samples, respectively. Based on analysis of coat protein (CP) nucleotide sequences, Australian BLRV isolates are closely related to each other and to the Argentine Manfredi isolate. Phylogenetic analyses based on CP gene nucleotide sequences confirmed separation of AMV isolates into two subgroups. The majority of AMV isolates, including all those from Argentina and Australia, clustered in subgroup I with isolates from various hosts and geographic origins. All Australian AMV isolates were closely related to AMV isolated from ADD-affected lucerne in Argentina.Fil: Samarfard, S.. The University Of Queensland; AustraliaFil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet. Córdoba. Unidad de Fitopatología y Modernización Agrícola (UFYMA); ArgentinaFil: Sharman, M.. The University Of Queensland; AustraliaFil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Dietzgen, R. G.. The University Of Queensland; Australi
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