4,623 research outputs found
Wavelength tunable 10-GHz 3-ps pulse source using a dispersion decreasing fiber-based nonlinear optical loop mirror
No full textWe experimentally demonstrate the use of a dispersion decreasing fiber (DDF)-based nonlinear optical loop mirror (NOLM) for the generation of wavelength tunable soliton-like pulses at a repetition rate of 10 GHz. We compress ~12 ps Gaussian pulses from an electro-absorption modulator (EAM) (followed by 125 m of DCF for preliminary linear dispersion compensation) into 3 ps pedestal-free pulses using both high-order soliton compression and nonlinear switching effects within an 8.5 km DDF-based loop mirror. The output pulses from the DDF-based NOLM show considerable pedestal reduction compared to those obtained by directly compressing the EAM seed pulses via a single passage through the DDF. Wavelength tuning of the compressed pulses over a ~15 nm bandwidth (from 1541 to 1556 nm) is demonstrated without a significant increase in pulse duration or degradation in pulse quality
Gill development in <i>Pseudopaludicola</i>.
No full textDetails of gill morphology and ciliation (left), embryos with gills at full development (middle), and gills regressing (right). (A) Ps. falcipes. (B) Ps. mystacalis. Scale bars 100μm. Note the overall poor development in the genus.</p
Measurements of the branchial sieve of sardine (Sardinops sagax ocellatus) from the west and south coasts of southern Africa
No full textIncludes abstract.Includes bibliographical references (p. 40-46).Branchial sieves of southern African sardine (Sardinops sagax ocellatus) were collected from three geographical regions along the southern African coast: Namibia, the south coast and the west coast of South Africa. Sardine from Namibia represented the northern Benguela upwelling ecosystem while sardine from the west and south coasts of South Africa represented the southern Benguela upwelling ecosystem. Morphometric measurements (gill arch length and gill raker spacing) and counts (number of gill rakers) of branchial sieves on the left side of the first gill arch were taken to determine whether the sardine from these regions have different branchial sieve structures
Player agency in interactive narrative: audience, actor & author
No full textThe question motivating this review paper is, how can
computer-based interactive narrative be used as a constructivist learn-
ing activity? The paper proposes that player agency can be used to
link interactive narrative to learner agency in constructivist theory,
and to classify approaches to interactive narrative. The traditional
question driving research in interactive narrative is, ‘how can an in-
teractive narrative deal with a high degree of player agency, while
maintaining a coherent and well-formed narrative?’ This question
derives from an Aristotelian approach to interactive narrative that,
as the question shows, is inherently antagonistic to player agency.
Within this approach, player agency must be restricted and manip-
ulated to maintain the narrative. Two alternative approaches based
on Brecht’s Epic Theatre and Boal’s Theatre of the Oppressed are
reviewed. If a Boalian approach to interactive narrative is taken the
conflict between narrative and player agency dissolves. The question
that emerges from this approach is quite different from the traditional
question above, and presents a more useful approach to applying in-
teractive narrative as a constructivist learning activity
Variation in susceptibility of laboratory and field strains of three stored-grain insect species to β-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces
No full textCitation: Sehgal, B., . . . & Gill, B. (2013). Variation in susceptibility of laboratory and field strains of three stored‐grain insect species to β‐cyfluthrin and chlorpyrifos‐methyl plus deltamethrin applied to concrete surfaces. Pest Management Science, 70(4), 576-587.
https://doi.org/10.1002/ps.3580The efficacy of commercial formulations of β-cyfluthrin and chlorpyrifosmethyl plus deltamethrin applied to clean, concrete surfaces similar to that of empty bins against field strains of stored-grain insects is unknown. We exposed adults of 16 strains of the red flour beetle, Tribolium castaneum (Herbst); 8 strains of the sawtoothed grain beetle, Oryzaephilus surinamensis (L.); and 2 strains of the lesser grain borer, Rhyzopertha dominica (F.), collected mainly from farm-stored grain in Kansas, USA, to β-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces and determined knockdown and mortality. RESULTS: Knockdown and mortality differences among species and strains to the insecticides tested were significant. Mortality of all species was less than that of knockdown, suggesting recovery when placed on food after insecticide exposure. β-cyfluthrin was effective against R. dominica but ineffective against T. castaneum and O. surinamensis field strains. Chlorpyrifosmethyl plus deltamethrin was only partially effective against field strains of the three species. CONCLUSION: Reduced susceptibility in field strains may be due to inherent formulation deficiency and low levels of tolerance or resistance to β-cyfluthrin. No single insecticide provided adequate control of the three species tested
Network analysis reveals significant joint effects of microplastics and tetracycline on the gut than the gill microbiome of marine medaka
No full textMicroplastics could accumulate and enrich antibiotics in the aquatic environment. Despite this, the joint effects of microplastics and antibiotics on aquatic organisms are not clear. Here, we investigated the changes of microbial interactions in both gill and gut of marine medaka exposed to polystyrene microbeads (PS) and/or tetracycline for 30 days by using co-occurrence network analysis based on 16 S rRNA gene amplicon sequences. We found that the single and combined effects of PS and tetracycline were more profound on the gut than on the gill microbiome. SourceTracker analysis showed that the relative contributions from the gill microbiome to the gut microbiome increased under combined exposure. Moreover, the combined exposure reduced the complexity and stability of the gut microbial network more than those induced by any single exposure, suggesting the synergistic effects of PS and tetracycline on the gut microbiome. The PS and tetracycline combined exposure also caused a shift in the keystone taxa of the gut microbial network. However, no similar pattern was found for gill microbial networks. Furthermore, single and combined exposure to PS and/or tetracycline altered the associations between the gut network taxa and indicator liver metabolites. Altogether, these findings enhanced our understanding of the hazards of the co-occurring environmental microplastics and antibiotics to the fish commensal microbiome
Adverse effects of environmentally relevant concentration of microplastics on gill epithelium permeability in the euryhaline Mediterranean killifish Aphanius fasciatus
No full textEstuaries and lagoons are characterized by fluctuating salinity and significant amounts of microplastics (MPs) and are increasingly subjected to various anthropogenic pressures. We investigated whether the accumulation of MPs in the gills of fish inhabiting these fragile ecosystems alters osmoregulation and, consequently, their ability to tolerate fluctuating salinity. The effects of a 15-day exposure to an environmentally relevant concentration (20 μg/L) of spherical polystyrene microplastics (PS-MPs) with a diameter of 5 μm were assessed in the Mediterranean killifish Aphanius fasciatus, focusing on tissue and gene expression changes related to factors of paracellular and transcellular permeability of the gill epithelium during the transition from seawater to freshwater. Our results revealed that PS-MPs indirectly impaired osmoregulation, particularly in fresh water, through their toxic effects on the gill tissue. Toxicity was evidenced by epithelial lifting, a decrease in the proportion of secondary lamellae available for gas exchange, and upregulation of superoxide dismutase and heat shock protein genes. Furthermore, exposure to PS-MPs directly affected gill epithelial permeability by maintaining relatively high paracellular permeability through the downregulation of claudin 3 and by modifying the expression of the transcellular transporter Na+/K+-ATPase and cystic fibrosis transmembrane conductance regulator in the gill epithelium. Overall, these findings confirm the toxic effects of PS-MPs on gill tissue and demonstrate, for the first time, that environmentally relevant concentrations of MPs adversely affect gill epithelium permeability during decreased salinity acclimation in the euryhaline fish A. fasciatus.This study was supported by: Ministry of Higher Education, Scientific Research and Technology of Tunisia and by ERASMUS+ mobility. The funding sources were not involved in the design of the study, or collection and analysis of data.Peer reviewe
Distraction: an assessment of smartphone usage in health care work settings
No full textPreetinder S Gill,1 Ashwini Kamath,2 Tejkaran S Gill31College of Technology, Eastern Michigan University, Ypsilanti, MI, USA; 2School of Information, University of Texas, Austin, TX, USA; 3College of Engineering, University of Michigan, Ann Arbor, MI, USAAbstract: Smartphone use in health care work settings presents both opportunities and challenges. The benefits could be severely undermined if abuse and overuse are not kept in check. This practice-focused research paper examines the current panorama of health software applications. Findings from existing research are consolidated to elucidate the level and effects of distraction in health care work settings due to smartphone use. A conceptual framework for crafting guidelines to regulate the use of smartphones in health care work settings is then presented. Finally, specific guidelines are delineated to assist in creating policies for the use of smartphones in a health care workplace.Keywords: smartphone, health care, distraction, workplace, mobile apps, health informatic
Charisma and Spirituality in the Early Church: A Study of Messalianism and Pseudo-Macarius
No full textThe thesis is an investigation into the concept of Charisma and Spirituality in the Early Church with particular emphasis upon the writings of Ps-Macarius, and of a group of ascetics known as the Messalians, evident in the late fourth / early fifth centuries. The Macarian writings are
examined to see what they reveal about the experiential pneumatic theology of the Early Church, the relationship between Syrian and Hellenic traditions of Christian Rhetoric, and the relationship between Ps-Macarius and the Cappadocian Circle. The Macarian corpus as a whole is examined to assess its rhetorical influences and style. The rhetoric of the Macarian corpus is seen to illustrate a high degree of sophistication. This study also gives definition to two terms that have become imprecise and diverse in their use: 'enkrateia' (self-control), and
`Syrian Christianity'. By isolating the characteristics of enkratefa the definitive stages of an encratic lifestyle are identified. The breaking down of the term into enkrateia, radical enkrateta and exclusive enkrateta enables a much clearer discussion to take place as to the
nature of the encratic theology of a group or individual. The final element of this study is a consideration of the distinct Macarian imagery that is evident within the corpus. Two images are considered in detail, the 'flight of the soul' and 'sober intoxication'. Overall this study
shows the variety of influences upon Ps-Macarius, and the uniqueness of his expression. The influences upon Ps-Macarius include a context of endemic Syrian spirituality, a radical encratic lifestyle, a Hellenic rhetorical training, and a distinct interpretation of Platonic and Neo-
Platonic images, coupled to the wider Judaic / Mesopotamian influences of his Church. It is shown that Ps-Macarius represents an individual voice that is distinct and recognisable amongst the Fathers of the Church
Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity
No full textL1-CAM (L1 cell-adhesion molecule), or more simply L1, plays an important role in the progression of human carcinoma. Overexpression promotes tumour-cell invasion and motility, growth in nude mice and tumour metastasis. It is feasible that L1-dependent signalling contributes to these effects. However, little is known about its mechanism in tumour cells. We reported previously that L1 is cleaved by ADAM (a disintegrin and metalloprotease) and that the cytoplasmic part is essential for L1 function. Here we analysed more closely the role of proteolytic cleavage in L1-mediated nuclear signalling. Using OVMz carcinoma cells and L1-transfected cells as a model, we found that ADAM10-mediated cleavage of L1 proceeds in lipid raft and non-raft domains. The cleavage product, L1-32, is further processed by PS (presenilin)/gamma-secretase to release L1-ICD, an L1 intracellular domain of 28 kDa. Overexpression of dominantnegative PS1 or use of a specific gamma-secretase inhibitor leads to an accumulation of L1-32. Fluorescence and biochemical analysis revealed a nuclear localization for L1-ICD. Moreover, inhibition of ADAM10 and/or gamma-secretase blocks nuclear translocation of L1-ICD and L1-dependent gene regulation. Overexpression of recombinant L1-ICD mediates gene regulation in a similar manner to full-length L1. Our results establish for the first time that regulated proteolytic processing by ADAM10 and PS/gamma-secretase is essential for the nuclear signalling of L1 in human carcinoma cell lines. Key words: a disintegrin and metalloprotease 10 (ADAM10), L1 cell-adhesion molecule (L1-CAM), nuclear translocation, presenilin (PS)/gamma-secretase activity, raft, signalling
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