189,801 research outputs found
Giemsa-stained impression smears of liver, spleen and brain revealing <i>T</i>. <i>gondii</i> tachyzoites.
No full text(A) Toxoplasma tachyzoites in infected untreated control liver (Giemsa stain X 1000). (B) Toxoplasma tachyzoites in infected treated liver (Giemsa stain X 1000). (C) Toxoplasma tachyzoites in infected untreated control spleen (Giemsa stain X 1000). (D) Toxoplasma tachyzoites in infected treated spleen (Giemsa stain X 1000). (E) Toxoplasma tachyzoites in infected untreated control brain (Giemsa stain X 1000). (F) Toxoplasma tachyzoites in infected treated brain (Giemsa stain X 1000).</p
Giemsa-stained bivalents.
No full textA. In the wild type, kinetochores (arrows) are often seen to project from chromosomes, indicating pulling by spindle microtubuli. B. This was never observed in the mutant when chromosomes were similarly compacted. Bivalents are not strictly arranged in tandem due to the breaking of the nuclear membrane caused by this method. Bar: 10 μm.</p
Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells
No full textTo obtain a useful rat liver cell line for in vitro carcinogenesis, two rat diploid epithelial cell lines were established from a 7-day-old male rat by the repeated colonial clone method. More than 80% of cells from each cell line have maintained normal diploid karyotype for over 30 months in vitro. The diploid cells were identi.
fied as normal diploid karyotype by conventional Giemsa and trypsin. Giemsa techniques. They showed little difference in morphology and growth rate between early and late passages. Without cloning, they tended to be heterogenous in cell morphology, became heteroploid
in chromosome and showed increased growth potential with time. Highly heteroploid cells which were derived from one of the lines produced ascites and solid tumors when inoculated into syngeneic rats intraperitoneally. Histologically, the tumors were diagnosed as poorly
differentiated hepatocarcinomas. One of these diploid epithelial cell lines in early passage contained some activity of tyrosine transaminase and liver type aldolase and .glycokinase. Therefore, it is suggested that these epithelial cell lines represent liver parenchymal cells.</p
Giemsa and C-banding karyotypes.
No full textGiemsa and C-banding karyotypes from Anavilhanas population (a-d)–cytotype A. Giemsa and C-banding karyotypes from Tupé population—cytotype B (e-h) triangles indicating terminal heterochromatic regions. Illustration by Lucas Kías.</p
Comparison of wet-mount, Wright-Giemsa and Gram-stained urine sediment for predicting bacteriuria in dogs and cats
No full textThis study assessed the standard urinalysis technique and sediment stain techniques as predictors of bacterial culture results for canine and feline urine. Canine (n = 111) and feline (n = 79) urine samples were evaluated using unstained wet-mount and air-dried Gram and Wright-Giemsa stained sediment; results were compared to aerobic bacterial culture. Eleven canine and 7 feline urine samples were culture positive. Unstained wet-mount and stained sediment had sensitivities of 89% and 83% and specificities of 91% and 99%, respectively. The specificity of using either stain was higher (P < 0.01) than wet-mount examination for detecting bacteriuria. There were significant differences among 3 technologists in detecting true positives (P < 0.01). Association of sediment and culture results used 112 canine and 81 feline samples. There was a negative association (P < 0.01) between lipid detection and wet-mount identification of bacteria.;Source type: Electronic(1
<i>P. murina</i> stained with rapid Wright-Giemsa.
No full text<p>Clusters of <i>P. murina</i> from a homogenate of an infected mouse lung were dropped on glass slides and stained with a rapid Wright-Giemsa. The black arrow points to a cluster of trophic forms. The white arrow indicates a mature cyst. The yellow arrow indicates an immature cyst with only three nuclei present in this section. The magnification bar represents 10 um. The micrograph was taken with an Olympus BH2 microscope and DP-72 digital camera.</p
Identifying different types of chromatin using Giemsa staining
No full textMixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli. After specific prestaining treatments that induce chromatin disorganization (giving banded or harlequin chromosomes), Giemsa staining produces a differential coloration, with C- and G-bands appearing in purple whereas remaining chromosome regions are blue. Unsubstituted (TT) and bromo-substituted (BT) DNAs also appear purple and blue, respectively. The same occurs in the case of BT and BB chromatids.<p></p>
In addition to discussing the use of Giemsa stain as a suitable method to reveal specific features of chromosome structure, some molecular processes and models are also described to explain Giemsa staining mechanisms of chromatin.<p></p>
Giemsa (top row) and proflavine (bottom row) stained leukocytes.
No full text<p>First row, Giemsa stained neutrophil (A), monocyte (B), lymphocyte (C). Second row, proflavine stained neutrophil (D), monocyte (E), lymphocyte (F). Images were captured at 60X. Fluorescence images were normalized as described in the Methods section. Scale bars = 10μm.</p
Giemsa-stained thin blood smear showing an immature schizont.
No full text<p>Giemsa-stained thin blood smear showing an immature schizont.</p
The appearance of basophilic stippling (objective 1.000x, Giemsa stain).
No full textThe appearance of basophilic stippling (objective 1.000x, Giemsa stain).</p
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