47,505 research outputs found
Translational research methods: Basics of renal molecular biology
No full textMolecular biology has developed between years 1940s and 1960s as a branch of science that spans from genetics to cell biology and biochemistry with the goal of understanding the interactions by which DNA, RNA, and protein synthesis operate into cells [1]. Since then, numerous technological innovations pushed the field forward, allowing recombinant DNA technology to be applied to a wide variety of biologic problems and to enter the clinical practice. The discovery of restriction nucleases and DNA ligases, development of DNA libraries, DNA cloning procedures, nucleic acid hybridization techniques, and the polymerase chain reaction (PCR) together with transgenic animals have all represented successive steps for a successful delineation of the role of genes in cellular physiology and pathophysiology. This molecular revolution has affected all of the sciences, including medical and clinical research, and has culminated in the completion of the human genome project [2, 3]. In the past 15 years, we have witnessed tremendous technological advances for high-throughput analysis of DNA, RNA, and protein in a comprehensive and cost-effective manner, with great promises to translate the analysis of the central dogma of biology into precision diagnosis and treatment for both rare and common diseases (Fig. 1). The recent advances in RNA biology identified numerous noncoding functional regions of the genome, which “escape” the central dogma but that can be analyzed and studied using a similar framework
Authors' Reply: "On the Importance of Considering Glycosylation when Evaluating Biologic Therapies"
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2de maps in the discovery of human autoimmune kidney diseases: The case of membranous glomerulonephritis
No full textThe latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples
No full textAlbumin heterogeneity in low-abundance fluids. the case of urine and cerebro-spinal fluid
No full textComparative study of thermal stability of healthy and focal segmental glomerulosclerosis plasma albumin
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