1,141 research outputs found
Hey bHLH Proteins Interact with a FBXO45 Containing SCF Ubiquitin Ligase Complex and Induce Its Translocation into the Nucleus
No full textThe Hey protein family, comprising Hey1, Hey2 and HeyL in mammals, conveys Notch signals in many cell types. The helix-loop-helix (HLH) domain as well as the Orange domain, mediate homo- and heterodimerization of these transcription factors. Although distinct interaction partners have been identified so far, their physiological relevance for Hey functions is still largely unclear. Using a tandem affinity purification approach and mass spectrometry analysis we identified members of an ubiquitin E3-ligase complex consisting of FBXO45, PAM and SKP1 as novel Hey1 associated proteins. There is a direct interaction between Hey1 and FBXO45, whereas FBXO45 is needed to mediate indirect Hey1 binding to SKP1. Expression of Hey1 induces translocation of FBXO45 and PAM into the nucleus. Hey1 is a short-lived protein that is degraded by the proteasome, but there is no evidence for FBXO45-dependent ubiquitination of Hey1. On the contrary, Hey1 mediated nuclear translocation of FBXO45 and its associated ubiquitin ligase complex may extend its spectrum to additional nuclear targets triggering their ubiquitination. This suggests a novel mechanism of action for Hey bHLH factors
Love in the First Degree: Manfred, Byron, and Incest
No full textThis is the author accepted manuscript. The final version is freely available from the University of Colorado via the link in this recordNote that the text of the manuscript varies considerably from the final published versionThis essay suggests that Byron’s Manfred contains not an expression of Byron’s guilt about his incest with his half-sister Augusta Leigh, as previous critics have suggested, but rather considerable evidence of his lack of guilt. It argues that the play displays incest and torment, but in fact does not link the two, instead displaying Manfred’s love for Astarte as deeply felt without regrets. The essay then argues that one finds the same combination of deep love and lack of regret in Byron’s remarks about his relationship with his half-sister, as well as in the representations of incest in his other works. It suggests that this acceptance of incest links to Byron’s commitment to rational thinking and personal freedom, and it invites future criticism to explore this connection in more detail
Manfred Macmillan
No full textDecadence meets gothic in Manfred Macmillan (1907), a carefully constructed tale of doppelgangers, magical intrigue, and the rootless scion of a noble house. This annotated, first-ever English translation presents an early queer novel long unavailable except in the original Czech. Author Jiří Karásek ze Lvovic (1871–1951) was a major cultural figure in his native Bohemia and cultivated ties with fellow artists from across Central Europe. In their extensive scholarly introduction, translator Carleton Bulkin and translation scholar Brian James Baer situate the novel within longer histories of gay literature, fascinations with the occult, and the cultural and linguistic politics of so-called peripheral European nations. They persuasively frame Karásek as a queer author and cultural disruptor in the fin de siècle Habsburg space.
Karasék rejected Czech translations of ancient Greek writers that bowdlerized gay themes, and he personally and vigorously defended Oscar Wilde in print, both on the grounds of artistic freedom and of private morality. He also published a cycle of homoerotic poems under the title Sodom, confiscated by the Austrian authorities but republished in 1905 and repeatedly afterward. A colonized subject, a literary decadent, and a sexual outlaw, Karasék’s complex responses to his own marginalization can be traced through his fantastically strange novel trilogy Three Magicians. As the first volume in that series, Manfred Macmillan is a gorgeous, compelling, and important addition to expanding canons of LGBTQI+ literature
Manfred Macmillan
No full textDecadence meets gothic in Manfred Macmillan (1907), a carefully constructed tale of doppelgangers, magical intrigue, and the rootless scion of a noble house. This annotated, first-ever English translation presents an early queer novel long unavailable except in the original Czech. Author Jiří Karásek ze Lvovic (1871–1951) was a major cultural figure in his native Bohemia and cultivated ties with fellow artists from across Central Europe. In their extensive scholarly introduction, translator Carleton Bulkin and translation scholar Brian James Baer situate the novel within longer histories of gay literature, fascinations with the occult, and the cultural and linguistic politics of so-called peripheral European nations. They persuasively frame Karásek as a queer author and cultural disruptor in the fin de siècle Habsburg space.
Karasék rejected Czech translations of ancient Greek writers that bowdlerized gay themes, and he personally and vigorously defended Oscar Wilde in print, both on the grounds of artistic freedom and of private morality. He also published a cycle of homoerotic poems under the title Sodom, confiscated by the Austrian authorities but republished in 1905 and repeatedly afterward. A colonized subject, a literary decadent, and a sexual outlaw, Karasék’s complex responses to his own marginalization can be traced through his fantastically strange novel trilogy Three Magicians. As the first volume in that series, Manfred Macmillan is a gorgeous, compelling, and important addition to expanding canons of LGBTQI+ literature
Introducing the Recent development in input-output analysis
No full textThis is a draft chapter. The final version is available in Recent Development in Input-Output Analysis, edited by Erik Dietzenbacher, Michael L. Lahr, and Manfred Lenzen, published in 2020, Edward Elgar Publishing Ltd. https://doi.org/10.4337/9781786430816.
The material cannot be used for any other purpose without further permission of the publisher, and is for private use only
Czech topics in work of Manfred Böckl
No full textDiese Abschlussarbeit beschäftigt sich mit tschechischen Themen in Werken des bayerischen Schriftstellers Manfred Böckl. Am Anfang werden das Leben und die Schöpfung dieses Autors vorgestellt, danach folgt die Analyse von tschechischen Themen bei ausgewählten Titeln. Die Arbeit ergänzt das Interview mit Manfred Böckl.Tato práce se zabývá českými tématy v dílech bavorského spisovatele Manfreda Böckla. Na začátku je představen život a tvorba tohoto autora, poté následuje analýza českých témat u vybraných literárních titulů. Práci doplňuje rozhovor s Manfredem Böcklem.This thesis is about czech topics in writings of bavarian author Manfred Böckl. In the first part there is described his life and literary output. After thet there is analysis of czech topics in selected literary works. Thesis is filled in with interview with Böckl himself.Katedra cizích jazykůDokončená práce s úspěšnou obhajobo
Survival in patients with high-risk prostate cancer is predicted by miR-221, which regulates proliferation, apoptosis, and invasion of prostate cancer cells by inhibiting IRF2 and SOCS3
No full textA lack of reliably informative biomarkers to distinguish indolent and lethal prostate cancer is one reason this disease is overtreated. miR-221 has been suggested as a biomarker in high-risk prostate cancer, but there is insufficient evidence of its potential utility. Here we report that miR-221 is an independent predictor for cancer-related death, extending and validating earlier findings. By mechanistic investigations we showed that miR-221 regulates cell growth, invasiveness, and apoptosis in prostate cancer at least partially via STAT1/STAT3-mediated activation of the JAK/STAT signaling pathway. miR-221 directly inhibits the expression of SOCS3 and IRF2, two oncogenes that negatively regulate this signaling pathway. miR-221 expression sensitized prostate cancer cells for IFN-γ-mediated growth inhibition. Our findings suggest that miR-221 offers a novel prognostic biomarker and therapeutic target in high-risk prostate cancer
Rohrthrips patrickmuelleri Ulitzka 2019
No full textRohrthrips patrickmuelleri Ulitzka, 2019: 32 Material studied. Holotype female MU-Fos-80/1 (Collection Ulitzka, Offenburg, Germany). Inclusion in Burmese amber, donated to the author by Patrick Müller.Published as part of Ulitzka, Manfred R., 2019, Addendum to MANFRED R. ULITZKA (2019) Five new species of Rohrthrips (Thysanoptera: Rohrthripidae) from Burmese amber, and the evolution of Tubulifera wings. Zootaxa, 4585: 027 - 040., pp. 596 in Zootaxa 4657 (3) on page 596, DOI: 10.11646/zootaxa.4657.3.12, http://zenodo.org/record/337192
A Pioneering Career in Catalysis: Manfred T. Reetz
No full textIn this invited Account, we highlight the enormous scientific breadth of our mentor Professor Manfred T. Reetz. It stretches from the development of organometallic reagents and transition metal catalysts to the adventurous idea of directed evolution of chemo-, stereo-, and regioselective enzymes, which he considered to be most important. We hope to show that Reetz did not consider these research areas to be totally unrelated realms, and attempt to reveal his transdisciplinary way of thinking about methodology development. Since biocatalysis has become crucial for chemical synthesis, we mainly focus on Reetz's contributions in this area. Some personal reflections from some of his former co-workers are also included, which reveal the stimulating atmosphere in the Reetz group in terms of science, career advice, and the importance of ethical considerations. BT/Biocatalysi
Themes of Isolation and Relationship in Selected Novels of Frederick Manfred
No full textThe initial appeal of the novelist Frederick Manfred for this student of American literature grew from the fact that he is a contemporary writer and that he lives in the Midwest, more precisely, in Siouxland. At first his novels seemed primarily entertaining, but interest in his work developed with an awareness of a parallel between Manfred\u27s concepts of the isolation of man and relationship to his fellow man and modern psychological ideas. Throughout the research and study of several novels, insights have crystallized to form a clearer idea of man\u27s search for identity and the results of that search in his life. Most of Manfred\u27s novels portray life in the early settlements on the plains of Siouxland. This is his home and the region he knows best. Many critics believe that he is one of the best novelists to write of-this period and setting. He has the ability to present realistically the thoughts of his characters and to give a feeling of the times he portrayed. Manfred has also expressed universality in the characters and their struggles. For example, the search of No Name, the young Indian lad in Conquering Horse, for his identity and purpose in life is a search every young man experiences. Two themes have emerged from a continued study of the novels and have become the focus for the research in this paper. Manfred shows in the lives of his characters two realities of human experience, an understanding of which seems essential to the healthy development of personality. These are the themes of isolation and relationship. Such a study in five selected novels of Manfred yields insight not only into Manfred\u27s characters but also into the struggle of every man to find himself. Furthermore, as the study has grown, it seems to explain the nature of Manfred as a person, as well as a writer. When the study was completed, the heroes in the novels were familiar but it was the character and thought of Frederick Manfred that had been revealed to this· writer. Particularly meaningful were his beliefs in individualism and relationship with others. The purpose of this thesis is to investigate the themes of isolation and relationships in five novels of Frederick Manfred. This provides a clearer understanding of the modern concept of the individual\u27s search for identity and an insight into the character of the author. The five novels chosen for this paper were selected on the basis of the insight they give into Frederic Manfred\u27s beliefs about man and how he discovers his own identity. Certain aspects of his writing led to the choice of these specific novels
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