79 research outputs found
Near-clinical applications of laser scanning cytometry
No full textBiological samples from human tissues are characterized by complexity and heterogeneity. The ability to make rapid, reliable, quantitative fluorochromatic measurements on clinical samples allows the development of new and practical assays that could influence diagnosis and treatment in a variety of clinical applications. Laser scanning cytometry (LSC) is a very versatile and adaptable technology that allows for the quantitative analysis of cell samples that are unsuitable for flow cytometry by virtue of their presentation and context. Crucially, it allows the direct visualization of cells and rare events and the correlation of imagery with fluorochromatic measurements. In this chapter, we describe early experiments in the study of cytotoxic drug uptake and resistance in human tumor cells and in the study of sputum cells from asthmatic patients, which harness the specific capabilities of LSC to practical clinical problems.</p
Image analysis enhancement of the laser scanning cytometer
No full textThe laser scanning cytometer offers a range of novel applications and the capacity for direct visual validation of experiments through sample analysis on a microscope slide. Linkage of the instrument to an image analysis system through standard connections and software enhances the capabilities of the instrument in image capture and manipulation. In this technical note, we describe a simple linkage between the LSC and the Kontron KS 100 Image Analysis System, an example of a standard commercial image processing instrument.</p
Erratum: Range-wide population genetic analysis of Seaside Sparrows (Ammospiza maritima) supports at least five distinct population segments that do not align with current subspecies descriptions (The Condor DOI: 10.1093/ornithapp/duab019)
No full textIn the originally published version of this manuscript, the author Stefan Woltmann was erroneously given affiliation 3, Florida Fish and Wildlife Conservation Commission, Lovett E. Williams Jr. Wildlife Research Lab, Gainesville, Florida, USA. Dr. Woltmann s correct information is affiliation 2, Department of Biology and Center of Excellence for Field Biology, Austin Peay State University, Clarksville, Tennessee, USA, and this has been updated in the original article. The publisher regrets this error
Neurogenic pulmonary edema presenting as a pulmonary entity
No full textAcute dyspnea is one of the most common presentations in acute/emergency settings, and acute pulmonary edema remains a leading cause in adults resulting from either cardiogenic or non-cardiogenic etiologies. Neurogenic pulmonary edema (NPE) is one of the less common forms of non-cardiogenic pulmonary edema seen in emergency departments, neurology units, or intensive care units. It usually develops rapidly following significant neurological insult seen in patients with intracranial hemorrhage, traumatic brain injuries, and epileptic seizures. It is less commonly seen after a multitude of other sudden catastrophic neurologic insults. Here, we report a case study of a 32-year-old female with a history of epilepsy since childhood who was admitted to our respiratory admission unit on two separate occasions with acute NPE and type I respiratory failure after a witnessed tonic-clonic seizure episode. Although the clinical features of NPE and the results of investigations can mimic more common cardiorespiratory conditions, an accurate and timely diagnosis is vital for the appropriate emergency management and to improve the patient's outcome.https://www.cureus.com/articles/100066-neurogenic-pulmonary-edema-presenting-as-a-pulmonary-entity#!
Survival following pulmonary rehabilitation in patients with COPD: the effect of program completion and change in incremental shuttle walking test distance
No full textLinzy Houchen-Wolloff,1 Johanna EA Williams,1 Ruth H Green,2 Gerrit Woltmann,2 Michael C Steiner,1,2 Louise Sewell,1 Michael DL Morgan,1,2 Sally J Singh1 1NIHR Leicester Biomedical Research Centre – Respiratory, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, 2Department of Respiratory Medicine, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, UK Rationale: Pulmonary rehabilitation (PR) in patients with COPD has consistently been shown to produce benefits in exercise capacity, symptoms, and health status. The data surrounding survival following PR are less clear. Our aims were to compare the long-term survival in two cohorts of patients referred for PR; those who successfully completed PR, and a comparator group constructed from patients who either did not complete PR or did not start the program. Additionally, we compared survival between those people who were able to achieve a clinically meaningful improvement in exercise capacity (incremental shuttle walking test) following PR with those who were not. Methods: A retrospective longitudinal analysis of clinical service outcomes was conducted to compare the long-term survival in “completers” and “non-completers” of rehabilitation at two hospitals within the University Hospitals of Leicester NHS Trust from January 1, 2000 to February 23, 2012. For “completers”, we also analyzed survival in those meeting (and not meeting) the desired level of change in the incremental shuttle walking test (≥50 m vs <50 m). Results: We present to you the largest dataset on this topic (n=1,515). Survival data were ascertained for 823 (54.3%) patients with COPD who had completed a course of PR and for 692 (45.7%) patients who dropped out. Survival time was significantly greater in “completers” compared to “non-completers” of PR (p<0.001). In addition, PR success (≥50 m change in walking distance) was also associated with improved survival (p<0.05). Conclusion: The data show an association between completion of PR and survival. In addition, PR success (>50 m change in walking distance) was also associated with improved survival. Keywords: survival, COPD, exercis
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What’s Past is Prologue: A Revolutionary Approach to Adaptations Studies
No full textIn this dissertation, I argue that adaptations studies can intersect with more traditional forms of literary theory, and that transformative adaptations are themselves a form of literary criticism. Using a revolutionary approach that interweaves different literary theories – African-American, psychoanalytic, queer, postcolonial, and postmodern – I make an intervention in contemporary scholarship (headed by Linda Hutcheon, Thomas Leitch, Julie Sanders, Cristina Bacchilega, Jack Zipes, and others) about adaptations studies to demonstrate its unlimited intertextual potentialities. Adaptations disrupt canonical hierarchies and create new forms of subjectivity that make possible different forms of empowerment and mastery than is present in their source texts. The transformative process takes place when an author changes a text by adapting it; however, it also occurs between texts, and from author to reader. Transformative indicates the ongoing process of these adaptations. Through revisionism, they affect the fundamental nature of their source text(s) and open authoritative narratives to questioning. The texts addressed in this dissertation are Alice Randall’s The Wind Done Gone; Jorge Luis Borges’ “The House of Asterion”; John Gardner’s Grendel; Malinda Lo’s Ash; Aime Cesaire’s A Tempest; Jean Rhys’s Wide Sargasso Sea; Jamaica Kincaid’s Annie John; and an assortment of parallel novels. These texts enact the transformative process through the rhetorical tools of collaborative originality, intertextual queering, and perspective plurality. They demonstrate the significance of intertextuality and the capabilities of literature as a form of empowerment. Reading the shifting meanings of these texts as adaptations shows the ideological transformations each undergoes and how they encourage an interactive readership. Adaptations that rewrite their source texts in a transformative way create a discursive web that allows for revolutionary approaches to literature and literary analysis
Transmucosal nasal drug delivery : pharmacokinetics and pharmacodynamics of nasally applied esketamine
No full textA Tale of Two Westphalia: The Narrative Evolution of a Historiographical Mythos from Nationalist History to Political Theory, 1808-1948
No full textThis paper examines the evolution of narratives on the Westphalian Treaties (1648) from nationalist German historiography in the nineteenth and early twentieth century to a political theory in the mid-twentieth century. Juxtaposing the narratives popularized by German Historians such as Karl Woltmann, Leopold von Ranke, and Heinrich von Treitschke to that of the Political Scientists Leo Gross and Hans Morgenthau, the Author seeks to explore how, and most importantly why, the narrative evolved. The paper demonstrates that the author’s personal experiences and political ideals, as well as contemporary realities are the primary drivers behind the evolution of a historical narrative, by focusing on shifts in the narrative tone and historical interpretation of Westphalia. The historical narrative thus resembles a kaleidoscopic image intertwining the past it seeks to depict with the authors’ realities. It also illustrates that historical narratives can transform dramatically and quickly once the ideological factors sustaining the narrative no longer resonate with an audience
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