216 research outputs found
Methodological aspects of the SAVE data set
No full textThis paper describes the general design of the SAVE survey: the design of the questionnaire, inter-viewer and interviewee motivation, and the sampling designs of the various subsamples collected in 2001 and 2003. It discusses the representativeness of the data, explains the construction of weights, and provides probit regressions to analyse potential selectivity problems. The paper finishes by discussing implications for the use of the SAVE data in various estimation procedures.
Clinical-scale isolation of ‘minimally manipulated’ cytomegalovirus-specific donor lymphocytes for the treatment of refractory cytomegalovirus disease
No full textAbstractBackground aimsReactivation of cytomegalovirus (CMV) after hematopoietic stem cell transplantation remains a major cause of morbidity despite improved antiviral drug therapies. Selective restoration of CMV immunity by adoptive transfer of CMV-specific T cells is the only alternative approach that has been shown to be effective and non-toxic. We describe the results of clinical-scale isolations of CMV-specific donor lymphocytes with the use of a major histocompatibility (MHC) class I peptide streptamer-based isolation method that yields minimally manipulated cytotoxic T cells of high purity.MethodsEnrichment of CMV-specific cytotoxic T lymphocytes (CTLs) was performed by labeling 1 × 1010 leukocytes from a non-mobilized mononuclear cell (MNC) apheresis with MHC class I streptamers and magnetic beads. Thereafter, positively labeled CMV-specific CTLs were isolated through the use of CliniMACS (magnetic-activated cell sorting), and MHC streptamers were released through the use of d-biotin. The purity of enriched CMV-specific CTLs was determined on the basis of MHC streptamer staining and fluorescence-activated cell sorting.ResultsA total of 22 processes were performed with the use of five different MHC class I streptamers. The median frequency of CMV-specific CTLs in the starting apheresis product was 0.41% among CD3+ T cells. The isolation process yielded a total of 7.77 × 106 CMV-specific CTLs, with a median purity of 90.2%. Selection reagents were effectively removed from the final cell product; the CMV-specific CTLs displayed excellent viability and cytotoxicity and were stable for at least 72 h at 4°C after MNC collection.ConclusionsClinical-scale isolation of “minimally manipulated” CMV-specific donor CTLs through the use of MHC class I streptamers is feasible and yields functional CTLs at clinically relevant dosages
The simultaneous isolation of multiple high and low frequent T-cell populations from donor peripheral blood mononuclear cells using the major histocompatibility complex I-Streptamer isolation technology
No full textImmunobiology of allogeneic stem cell transplantation and immunotherapy of hematological disease
Lothar Bickel : (8. Mai 1902 - 23. April 1951).
No full textThe author describes his friendship with Lothar "Elieser" Bickel in the Zionist youth group "Hashomer Hazair", where he met him in 1919 in Czernowitz, Bukowina. Discussion of Jewish-national and social problems and studies of Hebrew. Elieser's growing interest in philosophical and socialist themes. His brother Schlomoh Bickel was a leader of the worker's movement Poale Zion. Influence of the ethic seminary by Dr. Kettner and criticism on Zionist ideals. Elieser Bickel became acquainted with the philosopher Constantin Brunner and grew to become one of his most talented students. In 1922 Elieser enrolled at the Medical School in Bucharest, where he experienced virulent anti-Semitism at the university. Disintegration of Dr. Kettner's seminary in Czernowitz. Circle around Elieser Bickel who promoted the growing importance of Brunner's philosophy. In 1926 Elieser graduated. After completing his military service he decided to move to Berlin in 1927. Czernowitz philosophy circle in Berlin and friendship with Constantin Brunner. Lectures and studies of philosophy. Work as a physician in Berlin and Prenzlau. In 1931 journey to Spain. After Hitler's takeover in 1933 he moved back to Bucharest, where Lothar Bickel became one of the most renowned gynecologists. He continued his philosophic interests and specialized in the ethic of Spinoza and Kant. Death of Constantin Brunner in 1937. Acquaintance with Maedi Moscovici. They married in 1939 in Czernowitz. Military service and growing danger of approaching Germans. Precarious situation of the Jewish population. Armistice and continuation of his philosophic work. In 1950 Lothar Bickel emigrated to Canada. He died in Toronto in 1951.Lothar (Elieser) Bickel was born 1902 in Kisselew, a village in Northern Bukowina. He grew up in an orthodox house. His father was the owner of a small pub. Elieser was sent to his pious grandparents in Kolomea, where he received a pronounced Jewish education. He was sent to the high school (Gymnasium) and excelled as a student. After World War I and the collapse of the Austro-Hungarian Monarchy Kisselow became part of Romania. In 1918 Elieser continued his schooling at the Jewish Gymnasium in Czernowitz, where he was part of the Zionist youth group Hashomer Hazair. Elieser became a radical assimilationist under the influence of the philosopher Constantin Brunner. He studied medicine in Bucharest and settled as a physician in Berlin and Prenzlau. In 1933 Lothar Bickel returned to Bucharest. He emigrated to Canada in 1950, where he died one year later. Bickel wrote four philosophical books, which were published posthumously.Ball, SchlomohBrunner, LotteKasch, MagdalenaSemmel, KarlSonntag, LeoSternschuss, Mosche (Sterian)Stier, GabrielBucharestBerlinKolomeaAssimilationEducation, Jewish, 1871-1918Education, higher, 1918-1933FriendshipLiteratureMedicineMilitary servicePoale ZionYouth grou
Stanley's character polynomials and coloured factorisations in the symmetric group
No full textIn Stanley [R.P. Stanley, Irreducible symmetric group characters of rectangular shape, Sém. Lothar. Combin. 50 (2003) B50d, 11 p.] the author introduces polynomials which help evaluate symmetric group characters and conjectures that the coefficients of the polynomials are positive. In [R.P. Stanley, A conjectured combinatorial interpretation of the normalised irreducible character values of the symmetric group, math.CO/0606467, 2006] the same author gives a conjectured combinatorial interpretation for the coefficients of the polynomials. Here, we prove the conjecture for the terms of highest degree
Proposal to improve external communication of the Lothar Import SAS company before local and international costumers
No full textEsta tesis es un proyecto de investigación y desarrollo en cuanto a la comunicación externa de la empresa Lothar Import S.A.S, donde se crearon nuevos canales de comunicación como las redes sociales y su vez la traducción y mejoría de la página web. La metodología de este trabajo se hizo con el acompañamiento del gerente y la experiencia personal del autor de la tesis adquirida a lo largo del tiempo.This thesis is a research and development project in regard to the external communication of the Lothar Import S.A. S company, where it created new channels of communication, such as social networks and in turn the translation and improvement of the website. The methodology of this work was done with the accompaniment of the manager and the personal experience of the author of the thesis adquired over time.Profesional en Lenguas Moderna
Proposal to improve external communication of the Lothar Import SAS company before local and international costumers
No full textEsta tesis es un proyecto de investigación y desarrollo en cuanto a la comunicación externa de la empresa Lothar Import S.A.S, donde se crearon nuevos canales de comunicación como las redes sociales y su vez la traducción y mejoría de la página web. La metodología de este trabajo se hizo con el acompañamiento del gerente y la experiencia personal del autor de la tesis adquirida a lo largo del tiempo.This thesis is a research and development project in regard to the external communication of the Lothar Import S.A. S company, where it created new channels of communication, such as social networks and in turn the translation and improvement of the website. The methodology of this work was done with the accompaniment of the manager and the personal experience of the author of the thesis adquired over time.Profesional en Lenguas Moderna
Overconfident but yet well-calibrated and underconfident: A research note on judgmental miscalibration and flawed self-assessment*
No full textThe present paper addresses the question whether overconfidence is an individually stable phenomenon. A within-subjects design was used to investigate whether judgmental miscalibration also reflects tendency to make flawed self-assessments. While the former notion refers to the tendency of individuals to put unrealistic beliefs in their judgments, the latter concerns the tendency of individuals to make inaccurate evaluations of their abilities and performance. On the whole, the paper finds little support that those two tendencies should be related. Depending on the employed measurement, the participants were found to be simultaneously overconfident, well-calibrated, and underconfident.
The Uncontrolled Social Utility Hypothesis Revisited
No full textThe experiment disentangles communication and social effect in face−to−face communication. The results question the previous interpretation of communication effects in ultimatum bargaining, and suggest that separate processes, both of a strategic and of an affective−social nature induce cooperative outcomes.
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