39 research outputs found
Effect of Aegle marmelos seed extract on hypoglycemic activity of experimental rats
No full text29-32Aegle marmelos Corr. (Rutaceae) is widely used in Indian Ayurvedic medicine for the treatment of diabetes mellitus.
The aqueous extract of Aegle marmelos seeds was administered orally at different doses (100, 250 & 500 mg/kg) to
normal as well as sub (FBG normal; glucose tolerance abnormal) and mild (FBG 120-250 mg/dl) diabetic rats. Initial
testing was carried with the different doses of the seed extract in normal healthy rats fasted overnight. The animals
were divided into groups. Control rats (group I) were given distilled water only while other groups II, III and IV received
aqueous seed extract suspended in distilled water orally at doses 100, 250 and 500 mg/kg, respectively. The dose of
250 mg/kg was found to be most effective dose and it decreases blood glucose level (BGL) by 35.1 % in normal healthy
rats after 6 h of administration. The hypoglycemic effect of aqueous extract of A. marmelos seeds in sub and mild
diabetic rats was assessed by improvement of glucose tolerance. FBG was checked in overnight fasted rats and were
divided in to several groups. Control groups of sub and mild diabetic animals received distilled water only, whereas
variable doses of 100, 250 and 500 mg/kg of aqueous seed extract were administered orally to rest of the groups of
each, sub and mild diabetic animals. The rats of all the groups were given glucose (3 g/kg) after 90 min of the extract
and drug administration. Blood samples were collected just prior to glucose administration (0 h) and I, 2 and 3 h after
glucose loading. The dose of 250 mg/kg again showed a marked reduction in BGL of 41.2% in sub and 33.2% in mild
diabetic rats in glucose tolerance test after 2 h. The findings from this study suggest that the aqueous extract of these
seeds may be prescribed as adjunct to dietary therapy and drug treatment for controlling diabetes mellitus
Study of glycemic profile of Cajanus cajan leaves in experimental rats
No full text33-36Cajanus cajan K (Millsp) is commonly known as pigeon pea (or red gram). It is an important pulse crop of the tropical
regions. The present study deals with the aqueous extract of Cajanus cajan leaves and evaluation of its glycemic profile in
Streptozotocin induced type-2 diabetic rats. Single oral administration of graded doses of aqueous extract of leaves showed
significant increase in fasting blood glucose levels (FBG) of normal rats by 14.3%. The mild diabetic and sub-diabetic
animals had shown hyperglycemic effect from variable doses of extract. The maximum rise of 17.0, 71.3 and 50.8 % was
observed in BGL from a dose of 1000 mg/kg body weight after administration in normal, sub and mild diabetic rats,
respectively during glucose tolerance test (GTT). The study of leaves was taken into consideration on the basis of earlier
reported hypoglycemic activity of Cajanus cajan seeds. However, the results observed were found just opposite and
therefore it may be useful in controlling hypoglycemia, occasionally caused due to excess of insulin and other
hypoglycemic drugs
Design, development and synthesis of a novel labeled PNA monomer incorporated in DNA-hexamer to act as a hybridization probe by FRET
No full text121-129A novel PNA monomer with adenine nucleobase and a
modified backbone with charged and a methylene
substituted for in the linker
arm has been synthesised. This modified PNA monomer is further linked with
rhodamine via an amide bond, converted to phosphoramidite after linking with a
2-carbon linker. This unit is used for the end coupling at 5'-prime of a hexamer oligonucleotide 5'-TCC ATT-3' in solid phase. The
complementary labeled heptamer has been prepared by coupling the phosphoramidite
of fluorescein with 5'-AAT GGAT-3' as the end coupling step at 5'-prime on solid
phase. The two complementary heptamer oligos are hybridized and the FRET signal
recorded. The Tm of the modified oligomer has been found to be higher as
compared to the normal one. Such a probe can detect single base mismatch by
FRE
Role of Spectral Studies in Detection of Antibacterial Phytoelements and Phytchemicals of Moringa Oleifera
No full textThis paper reports, the Laser Induced Breakdown Spectroscopy (LIBS) studies and structure elucidation of compounds isolated from the fruit extract of Moringa oleifera and also deals with their possible effects on some bacterial strains viz. Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa. The extract was found to be active against all four microorganisms used. Extent of inhibitory effect of extract was assessed at different concentrations of 25, 50, 75 mg/ml by measuring diameter of inhibition zone (DIZ). Our results clearly showed that the 75 mg/ml concentration of the extract had 14, 12 and 18 mm of the DIZ against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa and 14 mm with 50 mg/ml concentration against Escherichia coli. The results were compared with the standard antibiotic ‘ampicillin’ of 1 mg/ml concentration. LIBS was recorded with high power pulsed laser beam from Nd: YAG Laser (Continuum Surelite III-10), focused on the surface of the material, which was in liquid form, to generate plasma on the surface of the sample. LIBS data clearly demonstrate the presence of trace elements, magnesium and iron, in high concentration in the extract. Whereas, from the phytochemical profile reveals the presence of two new compounds, S-ethyl-N-{4-[(a-L-rhamnosyloxy) benzyl]} thiocarbamate and 2-acetoxy {4-[(2',3',4'-tri-O-acetyl-a-L-rhamnosyloxy) benzyl]} acetonitrile as the major constituents. This study is the first report on synergetic effect of the phytoconstituents and certain set of elements present in their defined role in bacterial management against different bacterial strains
Role of Spectral Studies in Detection of Antibacterial Phytoelements and Phytochemicals of Moringa oleifera
No full textThis paper reports, the Laser Induced Breakdown Spectroscopy (LIBS) studies and structure elucidation of compounds isolated from the fruit extract of Moringa oleifera and also deals with their possible effects on some bacterial strains viz. Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa. The extract was found to be active against all four microorganisms used. Extent of inhibitory effect of extract was assessed at different concentrations of 25, 50, 75 mg/ml by measuring diameter of inhibition zone (DIZ). Our results clearly showed that the 75 mg/ml concentration of the extract had 14, 12 and 18 mm of the DIZ against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa and 14 mm with 50 mg/ml concentration against Escherichia coli. The results were compared with the standard antibiotic `ampicillin' of 1 mg/ml concentration. LIBS was recorded with high power pulsed laser beam from Nd: YAG Laser (Continuum Surelite III-10), focused on the surface of the material, which was in liquid form, to generate plasma on the surface of the sample. LIBS data clearly demonstrate the presence of trace elements, magnesium and iron, in high concentration in the extract. Whereas, from the phytochemical profile reveals the presence of two new compounds, S-ethyl-N-{4-[(alpha-L-rhamnosyloxy) benzyl]} thiocarbamate and 2-acetoxy {4-[(2',3',4'-tri-O-acetyl-alpha-L-rhamnosyloxy) benzyl]} acetonitrile as the major constituents. This study is the first report on synergetic effect of the phytoconstituents and certain set of elements present in their defined role in bacterial management against different bacterial strains
