1,721,199 research outputs found
Comparative study on milt quality features od different finfish species
Full text linkThe aim of this research was to study the main sperm characteristics of three different finfish species. Twenty-one gilthead
sea bream (Sparus aurata), 20 brown trout (Salmo trutta, morpha fario) and 15 rainbow trout (Oncorhynchus
mykiss) male broodstocks, farmed under optimal conditions for each species and fed standard diets for broodstocks, were
manually stripped. Brown trout yielded small amounts of sperm (4.5 vs 18.13 ml) that were very concentrated (≅ 8.5 x
109 vs 1.24 x 109 Szoa/ml) with respect to the other species. The duration of spermatozoan motility for gilthead sea
bream sperm was significantly longer (almost 50 min), in comparison to the one-minute motility of Salmonids. Single
fatty acids of brown trout sperm were higher than in the other two species for almost all detected fatty acids. In particular,
eicosapentaenoic acid (EPA) was at least three times more concentrated in brown trout than in rainbow trout or gilthead
sea bream sperm (1238.3 μg/g vs 305.6 and 333.3 μg/g, respectively; P < 0.01). Saturated, polyunsaturated and
total unsaturated fatty acid classes were significantly higher in brown trout sperm than in the other two species - almost
double with respect to gilthead sea bream sperm and more than double in comparison to RT sperm (P < 0.01)
Does dietary insect meal affect the fish immune system? The case of mealworm, Tenebrio molitor on European sea bass, Dicentrarchus labrax
Full text linkFeeding small European sea bass, Dicentrarchus labrax, for 6 weeks with Tenebrio molitor larval meal
showed significant anti-inflammatory responses (ceruloplasmin, myeloperoxidase and nitric oxide).
Serum bacteriolytic activity against a Gram negative bacterium was not significantly affected by dietary
Tenebrio, while both lysozyme antibacterial activity and serum trypsin inhibition usually linked to the
anti-parasite activity of the fish, were significantly enhanced. The latter may be due to the similarities in
the composition of the exoskeleton of parasites and insects that may therefore act as an immunostimulant
potentially increasing the anti-parasitic activity. The addition of exogenous proteases significantly
decreased both trypsin-inhibition and serum bacteriolytic activity probably through direct
inhibition of the proteins responsible for these immune functions. Further investigation involving bacterial
or parasitic challenges will be necessary to assess if the effects of dietary mealworm meal on the
immune system observed in the present study are translated into an improved resistance to diseases
J. Gasco , L. Carozza , R. Fry , J.-D. Vigne , J. Wainwright Le Laouret et la Montagne d'Alaric à la fin de l'Age du Bronze. Un hameau abandonné entre Floure et Monze (Aude)
No full textMordant Claude. J. Gasco , L. Carozza , R. Fry , J.-D. Vigne , J. Wainwright Le Laouret et la Montagne d'Alaric à la fin de l'Age du Bronze. Un hameau abandonné entre Floure et Monze (Aude). In: Bulletin de la Société préhistorique française, tome 94, n°1, 1997. pp. 22-23
Hepatic metabolites S-adenosyl methionine (SAM) and S-adenosyl homocysteine (SAH) as signals of methionine nutritional deficiency in rainbow trout (Oncorhynchus mykiss) fed on diets with partial replacement of fishmeal with insect (Hermetia illucens) meal
No full textInsect meal from Hermetia illucens in the diet of rainbow trout (Oncorhynchus mykiss): effects on growth and the expression of genes related to methionine metabolism.
No full textGrowth performance of common catfish (Ameiurus melas Raf.) fingerlings fed insect meal diets.
No full textIntestinal microbial communities of rainbow trout (Oncorhynchus mykiss) may be improved by feeding a Hermetia illucens meal/low-fishmeal diet.
Full text linkWith demands and reliance on aquaculture still growing, there are various challenges to allow sustainable growth and the shift from fishmeal (FM) to other protein sources in aquafeed formulations is one of the most important. In this regard, interest in the use of insect meal (IM) in aquafeeds has grown rapidly. Accordingly, the aim of the present study was to assess the effects of dietary IM from Hermetia illucens (Hi) larvae included in a low-FM diet on gut microbial
communities of rainbow trout (Oncorhynchus mykiss), in terms of both composition and function of microbiome. A feeding trial was conducted using 192 trout of about 100-g mean initial weight. Fish were fed in quadruplicate (4 tanks/diet) for 131 days with two diets: the control (Ctrl) contained 20% of FM as well as
other protein sources, whereas the Hi diet contained 15% of Hi larvae meal to replace 50% of the FM contained in the Ctrl diet. High-throughput sequencing
of 16S rRNA gene was used to identify the major feed and gut bacterial taxa, whereas Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis was performed on gut bacterial genomes to identify the major active biological pathways. The inclusion of IM led to an
increase in Firmicutes, mainly represented by Bacilli class and to a drastic reduction of Proteobacteria. Beneficial genera, such as Lactobacillus and Bacillus,
were enriched in the gut of fish fed with the Hi diet, whereas the number of bacteria assigned to the pathogenic Aeromonas genus was drastically reduced in the same fish group. The metagenome functional data provided evidence that dietary IM inclusion can shape the metabolic activity of trout gut microbiota. In particular, intestinal microbiome of fish fed with IM may have the capacity to improve dietary carbohydrate utilization. Therefore, H. illucens meal is a promising protein source for trout nutrition, able to modulate gut microbial community by increasing the abundance of some bacteria taxa that are likely to play a key role in fish health
Effects of dietary insect meal from Hermetia illucens on the expression of genes related to methionine metabolism in rainbow trout (Oncorhynchus mykiss)
No full textEffects of vitamin E and phosphatidylcholine on qualitative and quantitative parameters of rainbow trout (Oncorhynchus mykiss) milt
No full textThe aim of this work was to investigate the effects of vitamin E and phosphatidylcholine on rainbow trout
(Oncorhynchus mykiss) milt. One hundred and twelve rainbow trout (RB) broodstock (2n, 1030± 20g
body weight, male:female ratio = 50:50) were fed four isoproteic and isolipidic diets for 110 days. Diets
were differing for the type of vitamin premix and phosphatidylcholine supplied: Control (vitamin premix
without Vit. E, no phosphatidylcholine); Vit.E (a premix with Vit. E, no phosphatidylcholine); PhC, (vitamin
premix without Vit. E, phosphatidylcholine 2.5%); Vit.E +PhC, (vitamin premix with Vit. E and phosphatidylcholine
2.5%).
Sperm total volume, in sexually mature males (3+; 966±114g body weight), ranged between 18.57ml
(Control) and 34.31ml (Vit. E). Sperm density varied between 1.76x109 Szoa/ml (Control) and 1.16x109
Szoa/ml (Vit. E+PhC), while relative density (related to male body weight) tended to increase with Vit. E
(>50x109 Szoa/ml) and to reduce with Vit. E + PhC (85%
in all treatments, while motility duration was around 2.37min for Vit. E and Control reached only 0.97min.
After overnight storage (+4°C, for 18 hours) motility decreased, 75-80% in gamete motility and 3.39-
56.7% in time motility.
PhC dietary supplements significantly increased arachidonic acid contents of sperm with respect to
Control (>120 vs 73μg/ g), while Vit. E caused a huge increase in C20:3 n-3 (10.25 vs 2.27ppm).
DHA/EPA ratio was significantly lower in Control (>2; p<0.05), while n-3/n-6 ratio was significantly the
highest for Vit. E (9.46 vs <7.3)
Black soldier fly (Hermetia illucens) larvae meal as a dietary protein source for rainbow trout (Oncorhynchus mykiss): effects on allochthonous and autochthonous gut microbial communities.
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