1,721,037 research outputs found
ISOLATION, CHARACTERIZATION AND EXPANSION OF HUMAN LYMPHATIC ENDOTHELIAL CELLS IN VITRO.
No full textELM: a new, simple, and economic assay to measure motility of lymphatic endothelial cells
No full textBackground. Relative few attempts have been made to set up an assay that allows the measurement of lymphatic endothelial cells (LECs) motility. Nowadays the most widely used methods involve adaptation of the Boyden chamber method or the wound scratch assay, both of them showing some limitations due to long and expensive setup and high variability.
Methods and Results. We propose a new, economic, and Easy to setup LEC Motility (ELM) assay that will contribute to the study of lymphangiogenesis. The experimental set-up consists in extending the coating of the flask with extracellular matrix (ECM) proteins also at the area opposite to the cap, where the LECs will be initially seeded at various densities. The day after, the flasks will be inclined at an angle of about 20 degrees in order to cover the entire coated surface. 24 hours later, flasks will be moved to the standard position, and the motility of the cells will be easily observed. Using the ELM assay we were able to compare the motility rate of LECs isolated from different origins, or seeded on different substrates.
Conclusion. We propose the use of a new method to evaluate the motility of LECs: the ELM assay. This cost-effective analysis has several advantages: can be easily setup in any cell biology laboratory, can be carried out rapidly, and allows the monitoring of cellular motility for a long period
Lymph node derived lymphatic endothelial cells: isolation, purification, Characterization and long term culture
No full textRe “Calcification of Thoracic and Abdominal Aneurysms is Associated with Mortality and Morbidity”. Abdominal Aortic Aneurysm Calcification: Are Biochemical Markers a Missing Piece of the Puzzle?
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Increased frequency of Ureplasma urelyticum and Mycoplasma genitalium detection in AIDS patients without urethral symptoms.
No full textWhen fear backfires: Emergency department accesses during the Covid-19 pandemic
No full textAbstract
Introduction
The increase in access to Emergency Departments (ED) worldwide causes inefficiencies, but also signals its importance. The Coronavirus (Covid-19) outbreak allows to study the reactions of patients to the news about the spreading of the infection, which may have generated the fear that ED was no longer safe.
Methods
We study access to ED of a large teaching hospital in Brescia - one of the most hit provinces in Italy by Covid-19 - during the pandemic (from the announcement of the first cases to the explosion of the pandemic, to months after end of the acute phase) to study how patients reacted to the news that ED could no longer be a safe place. We analyse triage code, mode of arrival to ED, and accesses related to chest and abdominal pain, to evaluate who was discouraged most.
Results
Accesses have drastically reduced immediately after the news of the first contagion. During the lockdown accesses and admissions to hospital ward have decreased; this may mean that some patients may have suffered reduced health or increased mortality risks because of this decision. At the end of June accesses to ED and admissions to hospital ward are still lower than usual.
Discussion
Fear of contagion and appeals not to use ED directly by Covid-19 patients may have discouraged access also for pressing health need
Less is more: an ecological and economic point of view on appropriate use of lab testing for COVID patients
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