1,721,124 research outputs found

    Microplastics in Lake Champlain Fishes: Characterization and New Techniques

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    https://express.adobe.com/page/2KGtcuwMgDqDr/Microplastics, defined as being <5mm in size, have been recently identified as marine pollutants of significant concern. Concerns have arisen due to their persistence, ubiquity and potential to alter physiology and behavior that reduces reproduction and survival. As this is an emerging threat, the potential damage posed by microplastics to freshwater ecosystems has not yet been fully investigated and best practices for characterization are being developed. The purpose of this study was to learn the process of microplastics characterization in Lake Champlain and pilot the use of Nile red stain for quantification. In fall 2022, I dissected three fish (2 tench (Tinca tinca), 1 rudd (Scardinius erythrophthalmus)), isolated microplastics using wet peroxide oxidation, and characterizing them under the microscope. All particulate in fish were characterized as microfibers of small size (125-355um). Fiber load in tench, a larger fish, was 26% greater than that of rudd. Moving forward we plan to record this process for educational purposes and further develop a Nile Red staining procedure to expedite microplastic quantification.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Biota of Chazy Lake: The Legacy of Invasive Species and other Abiotic Stressors

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    https://docs.google.com/presentation/d/1REEcAsrX9ZnJL4mjY0zpKQSQeZFx2OTAgLouBuf2cXo/mobilepresent?slide=id.pFreshwater systems are threatened with poor water quality and invasive species, affecting their overall health. Chazy Lake is an oligotrophic impounded freshwater lake in Clinton County, New York approximately 1,800 acres in size and surrounded by mountains. Invasive species have become an increasing threat to the lake and include Eurasian watermilfoil (Myriophyllum spicatum), Chinese mystery snail (Cipangopaludina chinensis), and northern pike (Esox lucius). Abiotic stressors, such as road salt, are also major concerns. Over the course of four weeks in fall 2022, we surveyed the lake for fish and turtle community composition. At each site, hoop traps (n=2 minnow, n=1 turtle) were set at two reference (South Inlet, Pump station) and two disturbed (Dam, Seine Bay) sites. We created a Survey123 project to remotely georeference sites, curate images, and answer form questions. Water samples were collected to evaluate abiotic factors such as conductivity and pH. The species richness of the fish community was 5 and included 17 individuals including creek chub (Semotilus atromaculatus), pumpkinseed sunfish (Lepomis gibbosus), rock bass (Ambloplites rupestris), brook stickleback (Culaea inconstans), and yellow perch (Perca flavescens). Our hypothesis was not entirely supported as the majority of our fish were caught at the pump station (n=10) and disturbed Seine Bay (n=7) sites. None of the fish surveyed displayed disease phenotypes and no turtles were observed. Abiotic factors were surprising, as high conductivity (512 uS/cm) levels aligned with the Seine Bay, a site adjacent to a major roadway, while other sites averaged (94 uS/cm). Water chemistry revealed similar pH levels across sites 7.28-7.75. Non-profit organizations are addressing these threats with management efforts including lake drawdowns and seasonal watermilfoil removal. Long-term water quality monitoring has afforded residents opportunities to discuss alternatives and ways to minimize use of road salt. The lack of fish and turtles found in Chazy Lake may be just one sign of on-going threats associated with invasive species and pollution.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Comparing Herpetofauna Microbiome Diversity Across Northern New York

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    https://docs.google.com/presentation/d/1GVvjK5IyBS47D_vAudgo6Phs5C81WOkJfnwg-CkLxCM/mobilepresent?slide=id.pSeveral threats to herpetofaunal species such as habitat loss and the increase of diseases have decreased their global populations. Climate change is projected to shift many species of plants and animals into cooler regions. Within the last century there has been an ~80% decline in species due to habitat loss, climate change, and disease. Most notably is chytrid fungus, Batrachochytrium dendrobatidis, which keratinized the skin of herpetofauna. Microhabitat complexity is linked to their microbiome diversity and fitness. Our study was designed to evaluate the influence of both macro- and microhabitat on herpetofaunal epidermal microbiome. At three sites (Rugar Woods, Paul Smiths Visitor’s Interpretive Center, and Lewis Preserve Wildlife Management Area), we surveyed herpetofauna using hand capture techniques and collected microbial samples by swabbing the skin of individuals. Bacteria were plated to determine morphotype richness and serial dilutions were made in order to isolate the most prominent colonies. Microbial DNA was extracted, followed by a 16s rRNA polymerization chain reaction (PCR), and Sanger sequencing to confirm microbial species. Herpetofauna epithelial microbiome included Serratia sp., Pantoea sp., Pseudomonas sp., and Sphingobacteria. Herpetofaunal richness was the same across all macro-sites (S=3), with red-backed salamanders (Plethodon cinereus) being ubiquitous and spotted salamanders (Ambystoma maculatum), leopard frogs (Lithobates pipiens), spring peepers (Pseudacris crucifer), American toads (Anaxyrus americanus), and garter snakes (Thamnophis sirtalis) being rare. The most common microhabitat under which herpetofauna were found was coarse woody debris (CWD) and in terms of silviculture, single tree cuts and control sites had more animals than did other treatments such as clearcuts. Bacterial morphotype richness was greatest at Lewis Preserve and among red-backed salamanders and leopard frogs. Our bacterial species were common to herpetofaunal microbiomes and many support antifungal activity. Our findings suggested that a minimally managed wildlife management area with mature mixed forest, extensive floodplain, and riparian edge will support a diverse herpetofaunal community with high bacterial morphotype richness, affording greater defense against disease.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Assessing Small Mammal Richness and Abundance Following Wildfires

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    https://docs.google.com/presentation/d/1Er61wClF2qtcQ-mXgALafjSx6yN1767yCaXM3spJNzg/mobilepresent?slide=id.pAs with all disturbance, wildfire transforms the abiotic and biotic features of the landscape. The Altona Flat Rock is a globally rare sandstone pavement pine barrens ecosystem dominated by an overstory of Pinus banksiana (jack pine) and understory of ericaceous shrubs including Vaccinium augustifolium (blueberry). In summer 2018, a wildfire burned approximately 225 hectares of the jack pine barrens, a fire-dependent ecosystem. The small mammal community provides essential ecosystem services as seed predators, dispersers, and as prey for higher trophic levels. We aimed to determine the abundance and diversity of the small mammal community at two sites, specifically 1) the recent 2018 wildfire versus 2) a regenerated forest that burned in 1957. As part of an on-going mark-recapture study at the Flat Rock, small mammal live trapping was conducted over a six-week period in fall 2022. Each individual was uniquely marked with an ear tag and body metrics (length, weight) and gender were collected. Data from the 2022 field season was combined with previous years which showed that the small mammal abundance has declined over time and the reference (1957) site had higher community diversity including insectivores, while the recent burn (2018) had a higher overall abundance of the dominant generalist Peromyscus sp.. Results from this on-going study, can inform more effective management strategies in fire-dependent ecosystems by optimizing small mammal habitat and benefiting the ecosystem as a whole to better support the larger wildlife community and regenerating forest.NASUNY PlattsburghCenter for Earth and Environmental ScienceCenter for Earth and Environmental ScienceN/

    Effects of Landscape Context on Painted Turtle Population Structure in Northern New York

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    https://docs.google.com/presentation/d/1-MjjjiYUvoDDg6FTU3JeG7UE7JhWSUFrwSj2Jm8QRz0/mobilepresent?slide=id.pPainted turtles (Chrysemys picta) are a widely distributed freshwater turtle common in northern New York wetlands. Turtles provide ecosystem services that include redistributing nutrients, dispersing seeds, serving as prey to predators, and modifying wetland habitat. With ongoing threats due to climate change, disease, and losses in wetland habitat, painted turtle population health can reflect ecosystem health. We aimed to evaluate demographic and microbiome trends among painted turtle populations along an urbanization gradient in northern New York. In fall 2022, we set 10 hoop traps for 3 consecutive days at both an urban (Barracks golf course), Plattsburgh, NY and rural (Lake Alice Wildlife Management Area, Chazy, NY site. Following established Ecological Research as Education (TurtlePop 2.0) protocols. Gender and age structure of each individual was determined and each was uniquely marked using carapace scute notching techniques. We also evaluated Salmonella sp. presence on turtle’s at each pond by collecting both carapace and cloacal swabs. Site-specific characteristics were also noted at each pond including abundance of basking sites, pond area, water and air temperatures, pH, and conductivity. The greater abundance of turtles at the urban site could be explained by the presence of plentiful basking logs. Contrary to our hypotheses both urban and rural sites are female and adult skewed, which could be explained by site-specific mesopredator guild differences. Morphologically there were no major size differences noted between the urban and rural populations. Additionally, there were no significant differences in water quality between the pond complexes. Painted turtle carapace and cloacal microbiomes were dominated by Pseudomonas sp. and no salmonella bacteria were detected via Sanger sequencing. More long-term research needs to be done in order to determine the effects of urbanization on painted turtle populations as our findings appear in line with findings of the EREN network, but contrary to those of the larger body of turtle conservation research.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Wingin’ It: A Survey of Bat Populations in Varying Habitats in Northern New York

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    https://docs.google.com/presentation/d/1fpCwF4A4mZQ9rUvKQ_WOC1TGsHF8rpuzCJZjN-0Hy2w/mobilepresent?slide=id.g1971d8d6d1e_2_153Bats are taxonomically and ecologically diverse species who provide many ecosystem services. They are also sensitive to many anthropogenic stressors (e.g., urbanization, water quality, changes in climate), so changes in bat populations have historically been early sentinels of disturbance, facilitating focused conservation efforts. Unfortunately, following the introduction of white-nose syndrome (WNS) in 2006 in New York, the nation has seen variable but drastic declines in bat populations. The relationship between bats and their habitats is not well understood because they are wide-ranging and require multiple critical habitat types, but research is needed in order to meet conservation targets. In fall 2022, we assessed the abundance and diversity of bat species between forested, rural, and urban habitat types in northern New York. Each site was surveyed once using an ultrasonic bat detector thirty minutes after sunset, the most active feeding time for bat species. We hypothesized that bat abundance would be highest in the urban setting, with infrastructure providing increased roosting opportunity. However, highest species abundance and richness were both found at the forested site, likely because it was surveyed the earliest in the season. It was also the route with the most suitable habitat based on prior research suggesting bats need water for feeding and forests for roosting. The forested and rural sites showed 28.6% similarity, the rural and urban sites showed no similarity, and the forested and urban sites had 50% similarity. Understanding the disproportionate value that forest habitats provide for bat populations, we suggest considering conservation efforts which prioritize these ecosystems and link them to important riparian corridors.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Small Mammal Community Response to Wildfire at the Altona Flat Rock Sandstone Pavement Barren

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    The Altona Flat Rock is a sandstone pavement barren, dominated by the fire-dependent species known as Pinus banksiana (Jack Pine). Changes in seed availability, understory structure, and predator presence influence wildlife migration within the barren. Additionally, small mammal abundance often fluctuates cyclical in response to tree masting. In July 2018, a wildfire occurred at the Flat Rock pine barren. We aimed to monitor small mammal response to wildfire over the course of a year. Small mammal traps were set along established transects capturing the fire severity gradient and adjacent reference unburned area. Along those same transects, giving up density surveys (GUDS) were performed to foraging patterns in these varied microhabitats. We predicted greater capture rates and community diversity in the burn immediately post-fire due to access to the abundant serotinous Jack Pine seeds. In fall 2018 immediately following the wildfire, a total of 67 small mammals were captured with 1.5 times more in the unburned than burned area. The small mammal community consisted of Peromyscus spp. comprising 87% of captures and insectivores Sorex cinereus (Masked Shrew) and Blarina brevicauda (Northern Short-tailed Shrew) were absent from the burn. In fall of 2019, a total of 21 small mammals were captured with 3 times more in the burn than in unburned area. Community composition was exclusively Peromyscus spp. Over the course of a year, we noted a significant reduction in captures and a shift in microhabitat usage from unburned (2018) to burn (2019) likely in response to regenerating vegetation ameliorating predation risk. Interestingly, average body mass and total body length were higher in Peromyscus spp. in 2019, perhaps in response to increased seed predation. GUD survey results show seed foraging was 67% greater in 2018. Collaborators monitoring game cameras at the barren noted increased predator use of the unburned and burned areas in winter 2018 and spring 2019, respectively and a significant decline of predators from the area in late summer-fall 2019. A predator decrease in fall 2019 is paralleled with a significant decline in Peromyscus spp. This preliminary research has revealed the complexity of small mammal response to wildfire. Long-term monitoring will likely uncover their connection to resources, microhabitat structure, and predator abundance as regeneration continues.NASUNY PlattsburghCenter for Earth and Environmental ScienceN/

    Microplastic Biomagnification in Invertebrates, Fish, and Cormorants in Lake Champlain

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    Student poster, Center for Earth and Environmental Science, SUNY PlattsburghThe goal of this research was to determine whether microplastics (MP) are uptaken by invertebrates, fish, and Phalacrocorax auritus (double-crested cormorants) resident to Lake Champlain. We did so by quantifying and characterizing (e.g., fragment, fiber, film, foam, pellet) plastic particulate.Wet peroxide oxidation digests were performed on digestive tracts of 506 lake organisms, specifically invertebrates (n = 301), 15 species of fish (n = 190), and Phalacrocorax auritus (double-crested cormorants) (n = 15). Our research indicated that fibers were the were the most common (80.1%) type of particulate found in all organisms, followed by fragments (9.64%), films (6.36%), foam (3.01%), and pellets (Amia calva) contained the greatest average number of plastic particulate (n = 29.67), followed by lake trout (Salvelinus hamaycush) (n = 21.42), and northern pike (Esox lucius) (n = 20.1). Among digested fish, stomachs contained the greatest mean number of MPs (n=5.62), followed by the esophagus (n=5.36) and intestines (n=4.8). These findings suggest biomagnification and/or direct ingestion is occurring in Lake Champlain organisms, as invertebrates, fish, and double-crested cormorants contained on average 0.36, 6.08, and 22.93 microplastic particles

    Characterization of Microplastics using Fourier Transform Infrared Spectroscopy (FTIR)

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    Student poster, Center for Earth and Environmental Science, SUNY PlattsburghFourier transform infrared (FT-IR) is a spectroscopy technique widely used to analyze polymer profiles of particulate at a chemical level. The goal of this study was to assess the polymer composition of microplastics ingested by aquatic organisms from Lake Champlain. Preliminary results suggest fibers are the most prominent particle type in organisms (N = 482). Among these fibers, the most common plastic polymer was polyester [PET] (14.5%), followed by cellulose [20u ave particle size] (11.1%), alpha-cellulose [99.5% pure] (11.0%), and rayon (8.5%). Fragments were the second most prominent particle type (N = 168) and were commonly polyester [PET] (52%), followed by vinal (9%), polypropylene, isotactic (4%), and rayon (4%). Pellets (N = 14) were primarily vinylidene chlorine [200ppm mhdq] (14.2%) and polyethylene, chlorinated 36% chlorine (14.2%), followed by both vinal (7%), and cellulose nitrate (7%). Films (N = 11) were primarily rayon (27%), poly [methylmethacrylate] (27%), followed by poly [1,4-cyclohexanedimethylene terephthalate] (18%), and polypropylene, isotactic (9%). The least common polymer type found were foams (N = 10) comprised of polyethylene, chlorosulfonated (50%), polyethylene, chlorinated 36% chlorine (40%), and alzon [casein] (10%). Overall, polyester [PET] was more abundant as compared to other plastics and derives from synthetic clothing and food and beverage packaging.SUNY Plattsburg

    Bird Window Strike Monitoring at SUNY Plattsburgh

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    Student poster, Center for Earth and Environmental Science, SUNY PlattsburghBird window collisions are a major anthropogenically-derived threat, resulting in 100-1000 million bird deaths annually in the U.S., making it the second largest mortality factor for birds. The relationship between bird window collisions (BWC) and building factors, such as size, window area, proximity to nearest road (as well as traffic intensity on that road), and vegetation density surrounding buildings was studied. Six buildings, with different size and vegetation densities, were selected for this study. Daily carcass searches around each building were performed for 21 days, traffic intensity was determined via observation, and window area and vegetation density were calculated using ImageJ and ArcGIS respectively. Only one indicator of a BWC was found (a feather pile), thus there were not enough data to perform any correlation analyses between the factors mentioned above and BWCs based on the survey of SUNY Plattsburgh campus buildings alone. However, other BWC studies indicate that higher window area increases BWCs most strongly in areas of lesser development. This might be useful in focusing conservation efforts when planning major construction projects
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