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Immunohistochemical localization and identification of orexin A and orexin type 2 receptor in the horse gastrointestinal apparatus.
No full textPrimi risultati di uno studio istochimico dei glicoconiugati presenti nell’intestino di agnello
No full textCarbohydrate histochemistry of lamb duodenum
No full textIn order to elucidate the carbohydrate profile of the mucosa of lamb duodenum, conventional histochemical methods and a panel of 7 labelled lectins were used. In some cases, treatment with sialidase preceeded lectin staining. Carbohydrate histochemistry revealed the presence of sugar residues in the brush border of enterocytes, goblet cells and duodenal glands. All sites contained neutral and acid glycoconjugates. The presence of sulphomucins in goblet and duodenal gland cells was age-dependent. Enterocytes and duodenal gland cells contained abundant amounts of oligosaccharides with terminal sialic acid-galacto-syl(beta1 --> 3)N-acetylgalactosamine, whereas goblet cells contained the penultimate N-acetylgalactosamine residue linked to sialic acid. These findings were not age-dependent, whereas scarce amounts of fucose were found in all sites especially in young animals. The findings obtained in the present study serve as a basis for future pathological studies in lamb and sheep
Characterisation of sugar residues in glycoconjugates of pig mandibular gland by traditional and lectin histochemistry
No full textSugar residues are important components of salivary gland secretion. Traditional histochemical methods and lectin histochemistry were used to characterise glycoconjugates present in the mandibular gland of normal adult pigs. Acinar cells contained abundant quantities of glycoconjugates with the terminal trisaccharide sialic acid - (α2→3, 6) galactosyl (β1→3) N-acetylgalactosamine. Mandibular acinar cells also contained α and βN-acetylgalactosamine and N-acetylglucosamine residues, whereas the demilunar cells contained glycoconjugates with fucose. mannose and N-acetylglucosamine residues In the duct systen a range of sugar residues were localised throughout the cell cytoplasm or limited to the apical surface. These results provide new knowledge concerning the structure of salivary glycoconjugates in normal adult pig and a basis for future pathological studies
Glycoconjugate characterization in the intestine of Umbrina cirrosa by means of lectin histochemistry
No full textGoblet cells in the intestine of shi drum Umbrina cirrosa showed the presence of glycoconjugates particularly rich in fucose and N‐acetylglucosamine residues. They displayed also sialic acid linked to galactosyl(β1→3)N‐acetylgalactosamine and to galactosyl(β1→4)N‐acetylglucosamine. All the nine horseradish peroxidase‐conjugated lectins employed with the only exception of GSA II marked the enterocytes supranuclear region and the cell coat; the cell coat showed a more intense reactivity toward the different lectins, particularly enhanced with the use of fucosyl specific lectins
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) nel seme e nel testicolo do torelli di razza chianina, marchigiana e romagnola: un marker di potenziale fertilità?
No full textHistochemical characteristics of glycoconjugates of the carpal glands in domestic swine and wild swine
No full textHistology and ulstrastructure of the gastroenteric tract of the tilapia ( Tilapia spp), a hybrid teleost
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