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The 9th International Medicinal Mushrooms Conference - Book of Abstracts: ADVANCES IN MEDICINAL MUSHROOM SCIENCE: BUILDING BRIDGES BETWEEN WESTERN AND EASTERN MEDICINE
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Antioxidant properties, total phenolic and flavonoid content of different extracts of the medicinal mushrooms Pleurotus eryngii, Leccinum scabrum, Leccinum versipelle and Pisolithus tinctorius
Mushrooms consumption is increasing thanks to the presence in their composition of numerous bioactive compounds. As functional foods, mushrooms, represent a valid alternative to other foodstuff, for a balanced healthy nutrition for both humans and animals [1]. Medicinal mushrooms, represent a rich source of antioxidant compounds such as phenolic acids, flavonoids, carotenoids, and polysaccharides. In this survey, four species of medicinal mushrooms, Pisolithus tinctorius, Pleurotus eryngii, Leccinum scabrum, and L. versipelle were compared for their antioxidant activity and for the content of total phenolic compounds and flavonoids. Three different extracts were prepared for each mushroom using hot water (HW), ethanol (EtOH), and ethyl acetate (EA) as solvents for extraction. Each extract was tested for free radical scavenging activity, with different concentrations (from 20 mg/mL to 0.1 mg/mL), using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The radical scavenging activity was calculated as percentage of inhibition (%) measuring the absorbance of the blank at 517 nm and the absorbance of the extract sample. Butylated hydroxyanisole (BHA) was used as a positive control. All mushrooms extracts exhibited antioxidant activity, but P. tinctorius showed the highest DPPH radical scavenging activity for all the tested extracts and at all the concentration, with values ranging from 89.6±1.5 to 55.6±0.7 %, from 91.4±1.1 to 61.4±1.2 % and from 90.9±1.5 to 10.9±1.0% for HW, EtOH and EA, respectively.
Total phenols of extracts were determined according to the Folin-ciocalteu’s assay [2]. The absorbance was measured spectrophotometrically at 760 nm. The content of total phenols was calculated based on the calibration curve of Gallic acid and results were expressed as milligrams of gallic acid equivalents (GAE) per gram of mushroom extract. P. tinctorius exhibited the highest values of total phenolic content for HW and EtOH extract with value of 63.9±0.9, 71.6±0.4 GAE/g, respectively, while
L. scabrum and L. versipelle showed the highest values for AC extract (20.7±0.620.6±0.3 GAE/g, respectively). The quantification of total flavonoid content was conducted following the methodology of Kaewnarin et al., 2008, partially modified [3]. Absorbance was measured at 415 nm and the total flavonoid contents were calculated using a calibration curve of quercetin (QE) equivalents. Results were expressed as mg QE equivalents per gram of mushroom extract. P. eryngii EA extract showed the highest content of flavonoids (65.80±0.85 QE/g), while L. scabrum exhibited the highest values both for HW (17.70±1.13 QE/g) and EtOH (39.85±1.34
QE/g) extracts. Considering the high value of total phenolic compounds, but not of flavonoids, these results suggest that the highest antioxidant activity of P. tinctorius is probably due to other molecules which were not taken into consideration in this investigation, but which need further study in the future. In general, all four tested species exhibited good radical scavenging activity and therefore the possibility of being used as a functional food with high antioxidant properties
Ex situ conservation, enhancement and sustainable use of Pleurotus nebrodensis, a rare species, endemic to Sicily (southern Italy)
Pleurotus nebrodensis (Inzenga) Quél. is one of the rare examples of an endemic mushroom in Italy and at the European level. Previous studies have clarified the taxonomy of this taxon and the characteristics of its natural habitat [1, 2]. To safeguard the species, which is included in the EN (Endangered) category of the International Union for Conservation of Nature (IUCN) Red List of Threatened Species and among the TOP 50 Mediterranean Island Plants, in situ and ex situ conservation
actions have been proposed since 2000. These actions are also among the recent activities in SPOKE 3 “From alpha-diversity to citizen science” of the National Recovery and Resilience Plan and more specifically in Activity 3.2 “Assess and monitor endangered species/communities, as a consequence of climate change and anthropogenic perturbances”. In addition, in 2023, a project involving the cultivation of P. nebrodensis for food, medicinal and phytoiatric purposes was funded by the Region of Sicily under the 2014-2024 RDP Sicily. This project is aimed at ex situ cultivation of the rare mushroom in a protected environment. To date, in fact, this mushroom is only present in nature in small amounts and fruiting is gradually
declining due to the increase in the number of mushroom hunters and changing climatic conditions that are seriously affecting the conservation of the species. The Research Centers (CORIBIA, CORERAS), the Bari “Aldo Moro University and 7 farms are participating in the project. In addition to the direct partnership, the project will be supported by external partners such as GAL ISC Madonie, restaurants and GDO
«A chi diratti antico esempi lascia». La poesia di Garcilaso tra omaggio e sfida dei modelli
"Y si a mudarme a dar un paso pruebo". Discontinuità, intermittenze e durate nella poesia spagnola della modernità
"Decus et ordo": 'norma' e 'forma' nella città e nell'architettura della Roma post-avignonese
Il saggio introduce una panoramica topografica e architettonica della Roma del XV secolo, delle varie iniziative promosse-realizzate dai vari pontefici, a corredo dei luoghi interessati dal "Diluvio", citati dal testo di G.Dati.The essay offers a panoramic view of Rome during the XVth century in relation with the architectural and topographical papacy enterprises for the city: it is a sort of substain to the places advocated by the author G. Dati in his "Diluvio de Roma del MCCCCXCV..."
EPATITI B E C: MA I PAZIENTI CONOSCONO I RISCHI?
Gli infermieri hanno una funzione primaria nell’educazione sanitaria dei pazienti affetti da epatiti emotrasmesse, fondamentali risultano gli interventi di educazione sanitaria finalizzati a migliorare il grado di conoscenze, sia riguardo alle modalità di diffusione sia riguardo le misure profilattiche efficaci a ridurre l’incidenza di tali patologie. Un’indagine conoscitiva, condotta da infermieri italiani presso un ospedale in Spagna, ha permesso di definire, su un campione di 50 pazienti, il grado di conoscenze che i pazienti affetti da epatite B o C hanno, riguardo alle modalità di trasmissione dei virus stessi. I risultati hanno sostanzialmente evidenziato un discreto livello globale di conoscenze riguardo le modalità di trasmissione degli agenti patogeni oggetto di discussione
La stima del danno da incendi sul paesaggio boschivo del Gargano
L’obiettivo generale della ricerca è verificare se, ed in che misura, gli incendi boschivi comportino nella zona del Parco Nazionale del Gargano un peggioramento rilevante della percezione estetico-visiva del paesaggio. In particolare, lo studio mira ad analizzare la percezione sociale del paesaggio garganico e a stimare la “disponibilità a pagare” per scongiurare il danno paesaggistico derivante dallo sviluppo di incendi boschivi.
A tal fine sono stati impostasti due esperimenti di valutazione contingente, rivolti all’analisi delle preferenze dei residenti della provincia di Foggia e dei turisti extra-provinciali. Le due indagini sono state progettate e condotte per rispondere ad esigenze di natura diversa.
L’indagine rivolta alla popolazione residente mira, principalmente, alla stima della disponibilità a pagare monetaria ed all’analisi delle componenti socio-economiche che condizionano il valore monetario attribuito dalla collettività, al fine di fornire elementi di riflessione utili all’Ente Parco Nazionale del Gargano nella definizione di politiche di gestione e di tutela del paesaggio.
Quella rivolta ai turisti, invece, ha soprattutto carattere esplorativo e si rende necessaria in quanto, non essendo disponibili dati sui flussi turistici reali, non si dispone di alcuna informazioni sulle caratteristiche socio-economiche ed attitudinali dei turisti e sulle loro preferenze.
Anche in questo caso sono state raccolte informazioni utili alla stima della disponibilità a pagare per tutelare il paesaggio garganico dal danno derivante dagli incendi boschivi. Tuttavia, la limitatezza del campione intervistato non consente di effettuare le elaborazioni econometriche impiegate per la stima delle preferenze monetarie dei residenti. In ogni caso, i dati raccolti ed i risultati conseguiti appaiono conformi agli obiettivi prefissati
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