200,060 research outputs found
Monitoring Cl- movement in single cells exposed to hypotonic solution
No full textSelf-referencing ion--selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1-3 microm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials (VN) measured in 100 and 10 mM NaCl (deltaVN = 57 +/- 2 mV), but was slightly greater than ideal when comparing 1 and 10 mM NaCl (deltaVN = 70 +/- 3 mV). The response was also linear in the presence of 1 mM glutamate, gluconate, or acetate, 10 microM tamoxifen, or 0.1, 1, or 10 mM HEPES at pH 7.0. The ISE was approximately 3 orders of magnitude more selective for Cl- over glutamate or gluconate but less than 2 orders of magnitude move selective for Clover bicarbonate, acetate, citrate or thiosulfate. As a result this ISE is best described as an anion sensor. The ISE was 'poisoned' by 50 microM 5-nitro-2-(3phenylpropyl-amino)-benzoic acid (NPPB), but not by tamoxifen. An outward anion efflux was recorded from cells challenged with hypotonic (250 +/- 5 mOsm) solution. The increase in efflux peaked 7-8 min before decreasing, consistent with regulatory volume decreases observed in separate experiments using a similar osmotic protocol. This anion efflux was blocked by 10 microM tamoxifen. These results establish the feasibility of using the modulation of electrochemical, anion-selective, electrodes to monitor anions and, in this case, chloride movement during volume regulatory events. The approach provides a real-time measure of anion movement during regulated volume decrease at the single-cell leve
C. M. Garber photograph
No full textProfessor C. M. Garber, ca. 1930-1955. Garber spent many years in the Arctic sharing the life of western Eskimos. He is shown here wearing traditional arctic skins and furs
James R. Garber correspondence, MSS.0557
No full textAbstract: Personal and professional letters to and from Dr. James R. GarberScope and Content Note: The collection contains letters to and from Dr. James R. Garber concerning topics both personal and touching on his profession as a doctor. There are also letters of recommendation (and letters requesting favorable letters of recommendation) for entrance to medical school and internships to various hospitals.Biographical/Historical Note: James M. Garber was a doctor in Birmingham, Alabama, in the first half of the twentieth century. He was also at one time the Chairman of the Board - Medical Director fo the Physicians' National Life Insurance Co. He and his wife, Bessie, had two daughters, Katherine and Mary. Dr. Garber died in 1978
Writer and sculptor John Garber has owned Sequin, or Seguin Island for over a de
No full textWriter and sculptor John Garber has owned Sequin, or Seguin Island for over a decade. He comments on its camps, shores, and trees
Letter from Brigadier General B. L. Holt, Montgomery, Alabama, to Colonel Alex M. Garber, Montgomery, Alabama, August 25, 1908
No full textAn item from the papers of Barrie Lucien Holt. These papers cover Holt's life as a capitalist, planter, financier and Quartermaster General of the Alabama National Guard. Holt was an organizer and majority shareholder in the Prattville Cotton Mills and Banking Company, as well as a partner in D.M. Snow and Company which owned a hardware store and engaged in money-lending
Accn2505_001_036
No full textSeries of letters from 1966 to 1975 between Dwight W. Garber of Perrysville, Ohio, and Stockton, California, and Virgil M. Webb of Salt Lake City, concerning people involved in the Haun\u27s Mill episode and the Mormon Battalion. Includes a copy of Dr. Seymour B. Young\u27s article, "A visit to Missouri," from Improvement Era, vol. 24, no. 2 (December 1920)
Some identities involving second kind Stirling numbers of types B and D
No full textUsing Reiner’s definition of Stirling numbers of the second kind in types B and D, we generalize two well-known identities concerning the classical Stirling numbers of the second kind. The first identity relates them with Eulerian numbers and the second identity interprets them as entries in a transition matrix between the elements of two standard bases of the polynomial ring R[x]. Finally, we generalize these identities to the group of colored permutations G(m,n)
Evidence of Alternative Dietary Syndromes and Nutritional Goals in the Genus Alouatta
No full textHowler monkeys exploit diffi cult-to-digest and potentially toxic food items such as mature leaves and unripe fruits; persist across an extreme range of habitat types, including highly disturbed forests; and have the most widespread geographical distribution of any genus of New World primate. Given evidence of dietary variability in the amount of monthly fruit, leaf, and fl ower consumption, howler monkeys provide an instructive model for examining relationships among foraging strategies, activity budgets, and patterns of habitat utilization. In this chapter we examined evidence for interspecifi c differences in dietary patterns and nutritional ecology within the genus Alouatta and identifi ed three dietary ?syndromes? that are generally consistent with howler monkey phylogeny and biogeography. Specifically, we show that Mesoamerican howler monkeys and A. seniculus are characterized by a balanced leaf and fruit diet, Amazonian species by a fruit enriched diet, and Atlantic Forest and southern howler monkeys by a leaf-enriched diet. Finally, to be able to identify species-specifi c dietary strategies and syndromes across the primate Order, we recommend an approach that includes collecting data on feeding rates and the nutritional composition of the diet.Los monos aulladores consumen alimentos difíciles de digerir y que potencialmente contienen compuestos secundarios tóxicos como hojas maduras y frutos inmaduros; habitan en tipos de ambientes muy variados, incluyendo áreas altamente fragmentadas, y tienen la distribución geográfica más amplia de cualquier otro género de primates neotropicales. Debido a las conspicuas fluctuaciones mensuales en las cantidades de frutos, hojas y flores consumidos, los monos aulladores constituyen un modelo útil para examinar las relaciones entre estrategias de forrajeo, patrones de actividad y de utilización de hábitat en otros primates, incluyendo a los folívoros. En este capítulo examinamos la evidencia de diferencias interespecíficas en los patrones alimentarios y nutricionales dentro del género Alouatta, e identificamos tres “síndromes” alimentarios consistentes con los patrones filogenéticos y biogeográficos. En particular, mostramos que los monos aulladores de Mesoamérica y A. seniculus se caracterizan por tener una dieta balanceada de hojas y frutos, las especies del Amazonas por una dieta en la que predominan los frutos, y aquellas de la Foresta Atlántica y del Sur por una dieta en la que predominan las hojas. Finalmente, subrayamos la importancia de colectar datos sobre las tasas de ingestión de los alimentos y sobre la composición nutricional de la dieta para poder identificar estrategias y síndromes alimentarios especie-específicos en los primates.Fil: Garber, Paul A.. University of Illinois; Estados UnidosFil: Righini, Nicoletta. University of Illinois; Estados UnidosFil: Kowalewski, Miguel Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentin
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