12 research outputs found
Butǐ e rigitkone (sevitkone) siklǎrnesqëri dre rromane ćhavenqëro siklǎripen – eksperiènce wrocławitkone śkòlenθär
No full textThe author presents problems which she encounters in her work as a teacher who assists the education of Roma children in Wrocław. She mainly emphasizes, however, how Romani children, young people and whole families benefit from the work of such a teacher who is an intermediary between the school and Roma community.I avtòra sikavel nesave problème so arakhlǎs pe pesqëro drom kana kerel sas butǐ sar siklǎrni savi del tato vast e rromane ćhaven and-o Wrocław. Joj buteder thovel emfàza maśkar averenθe sar rromane ćhave, ternimàta thaj saste irrǎ (famìlie) xutren laćhipen (hàzna) gasave siklǎrnesθar, savo si maśkarno maśkar-i skòla thaj o rromano khetanipen.Natalia Gancar
Development and validation of UHPLC-ESI-MS/MS bioanalytical method, ADMET profiling, and pharmacokinetic study of bioactive phytoconstituents from Ayurvedic botanical Guduchi (Tinospora cordifolia)
No full textTinospora cordifolia (TC) is known for its immense therapeutic applications in 'Ayurveda', which has created considerable scientific interest in pharmacology. Thus, a targeted and rapid bioanalytical UHPLC-ESI-MS/MS method was developed and validated for the extraction of its bioactive markers from diverse classes of diterpenes as tinosporide (TC1) and phytosterol-20-β-hydroxyecdysone (TC2) and isoquinoline alkaloids-jatrorrhizine (TC3), tetrahydropalmatine (TC4) from the TC stem extract (TCE) in rat plasma by solid phase extraction technique (SPE). The optimum recovery (≥ 90 %) was achieved for TC1–4 and internal standard fluoxymesterone (IS) with the SPE method on the C18 phase. The analytes were subjected to chromatographic analysis on the Agilent C18 Zorbax Eclipse Plus column (4.6 × 100 mm, 3.5 µ) with a gradient program using 0.1 % acetic acid in water (% v/v) and acetonitrile as mobile phase at a flow rate of 0.500 mL/min. The MS/MS quantification and validation were performed on the Shimadzu 8045 tandem mass spectrometer associated with the heated-ESI probe in SRM mode. The precursor to product ion transitions m/z 416.20→375.10 (TC1), 481.40→445.20 (TC2), 339.15→323.05 (TC3), 356.25→192.10 (TC4) and 337.20→91.00 (IS) were used for quantification. Also, in silico ADMET prediction and in vivo pharmacokinetics revealed that TC1–4 was well absorbed from the GI tract and could act as a P-GP substrate. In the pharmacokinetic study, TC1–4 could be detected by this validated bioanalytical method. The TC1 was found bioavailable, having an optimum half-life of > 9.0 h to exhibit therapeutic activity with other TC bioactive markers in vivo. This research is the first comprehensive study on in silico and in vivo pharmacokinetics of TC biomarkers, which may aid in further pre-clinical and clinical trial investigations
Optimization of HPTLC densitometric method for curcuminoids and polyphenolics in an ayurvedic Emblica officinalis and Curcuma longa based Nishamalaki formulation by Box-Behnken design
No full text719-728The present study focuses on developing a simplified, specific, and accurate high performance thin layer chromatographic (HPTLC) method for the quantitative and qualitative determination of ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) in Nishamalaki Ayurvedic formulation. Pre-coated silica gel 60 F254 aluminum-backed plates were used as the stationary phase in the chromatographic technique development, and the optimized mobile phase was toluene: dichloromethane: glacial acetic acid: formic acid (6:4:1.6:0.9% v/v/v/v) with double development in linear ascending mode. The detection wavelength for quantification for ellagic and gallic acid was 280 nm, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) were 430 nm. The optimized mobile phase showed optimum separation between peaks for ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) at RF of 0.12±0.02, 0.21±0.02, 0.55±0.02, 0.69±0.02 and 0.82±0.02 respectively. Chromatographic conditions were optimized using the Box-Behnken design. Various variables, such as, the volume of formic acid and glacial acetic acid, and chamber saturation time, that are likely to impact RF were identified for further optimization. The volume of glacial acetic acid may be regarded as a critical method parameter, which caused the greatest change in the RF value and was the important factor among the three factors. The linear range was 600-1800 ng/band for all markers (r2 greater than 0.98). The limit of detection (LOD) and quantification (LOQ) measured indicated the method’s sensitivity. For all markers, the recovery percentage reveal acceptable accuracy, and the method was repeatable and reproducible from precision measurements with less than a 2% relative standard deviation. The optimized method was precise, specific, accurate, robust and reproducible for quantifying ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) in the quality-control testing of botanical extract along with Nishamalaki ayurvedic formulation
Distinct Morphological and Analytical Features of <i>Spinacia oleracea</i> Differentiating from Conventional Spinach Plants
No full textSpinacia oleracea (S. oleracea), also known as spinach or Indian spinach, is a rich source of carotenoids
and flavonoids with a wide range of health benefits. Besides S. oleracea, many other plants are also known as spinach
or its common versions, which could be misleading in the identification
of S. oleracea in any nutraceuticals and dietary
supplements. Thus, the present study aims to differentiate S. oleracea from other conventionally known spinach plants
by its macroscopic, microscopic, and phytochemical features. Although
these plants were morphologically distinct, analytical investigations
were useful in differentiating S. oleracea from other
spinach plants in dried or powdered forms. In the present study, HPTLC
fingerprinting could efficiently differentiate between these plants
based on the isoflavones present in the leaves of S. oleracea. Followed by a robust and simple HPLC-PDA method that was validated
for 20β- hydroxyecdysone and isoflavones as authenticating markers
with kaempferol from S. oleracea leaves for quality
control. These selected bioactive markers specific to S. oleracea were present in the range of 3.101–1232.820 mg/kg and were
found absent in spinach samples. Essentially, the described set of
methods could be used for species authentication and standardization
of S. oleracea differing from other plants known
as spinach
Bioanalytical Method Development and Validation Study of Neuroprotective Extract of Kashmiri Saffron Using Ultra-Fast Liquid Chromatography-Tandem Mass Spectrometry (UFLC-MS/MS): In Vivo Pharmacokinetics of Apocarotenoids and Carotenoids
No full textKashmir saffron (Crocus sativus L.), also known as Indian saffron, is an important Asian medicinal plant with protective therapeutic applications in brain health. The main bioactive in Kashmir or Indian Saffron (KCS) and its extract (CSE) are apocarotenoids picrocrocin (PIC) and safranal (SAF) with carotenoids, crocetin esters (crocins), and crocetins. The ultra-fast liquid chromatography(UFLC)- photodiode array standardization confirmed the presence of biomarkers PIC, trans-4-GG-crocin (T4C), trans-3-Gg-crocin (T3C), cis-4-GG-crocin (C4C), trans-2-gg-crocin (T2C), trans-crocetin (TCT), and SAF in CSE. This study’s objectives were to develop and validate a sensitive and rapid UFLC-tandem mass spectrometry method for PIC and SAF along T4C and TCT in rat plasma with internal standards (IS). The calibration curves were linear (R2 > 0.990), with the lower limit of quantification (LLOQ) as 10 ng/mL. The UFLC-MS/MS assay-based precision (RSD, <15%) and accuracy (RE, −11.03–9.96) on analytical quality control (QC) levels were well within the acceptance criteria with excellent recoveries (91.18–106.86%) in plasma samples. The method was applied to investigate the in vivo pharmacokinetic parameters after oral administration of 40 mg/kg CSE in the rats (n = 6). The active metabolite TCT and T4C, PIC, SAF were quantified for the first time with T3C, C4C, T2C by this validated bioanalytical method, which will be useful for preclinical/clinical trials of CSE as a potential neuroprotective dietary supplement
Development of validated UHPLC–PDA with ESI–MS-MS method for concurrent estimation of magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine in Berberis aristata
No full textInvestigating 11 Withanosides and Withanolides by UHPLCPDA and Mass Fragmentation Studies from Ashwagandha (Withania somnifera)
No full textPharmacokinetic Study of Withanosides and Withanolides from Withania somnifera Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS)
No full textWithania somnifera is a traditional Indian herb described under the ‘Rasayana’ class in Ayurveda, which gained immense popularity as a dietary supplement in the USA, Europe, Asia, and the Indian domestic market. Despite enormous research on the pharmacological effect of withanosides and withanolides, bioanalytical method development and pharmacokinetics remained challenging and unexplored for these constituents due to isomeric and isobaric characteristics. In current research work, molecular descriptors, pharmacokinetic, and toxicity prediction (ADMET) of these constituents were performed using Molinspiration and admetSAR tools. A rapid, selective, and reproducible bioanalytical method was developed and validated for seven withanosides and withanolides as per USFDA/EMA guidelines, further applied to determine pharmacokinetic parameters of Withania somnifera root extract (WSE) constituents in male Sprague Dawley rats at a dose of 500 mg/kg. Additionally, an ex vivo permeability study was carried out to explore the absorption pattern of withanosides and withanolides from the intestinal lumen. In silico, ADMET revealed oral bioavailability of withanosides and withanolides following Lipinski’s rules of five with significant absorption from the gastrointestinal tract and the ability to cross the blood-brain barrier. Upon oral administration of WSE, Cmax was found to be 13.833 ± 3.727, 124.415 ± 64.932, 57.536 ± 7.523, and 7.283 ± 3.341 ng/mL for withanoside IV, withaferin A, 12-Deoxy-withastramonolide, and withanolide A, respectively, with Tmax of 0.750 ± 0.000, 0.250 ± 0.000, 0.291 ± 0.102, and 0.333 ± 0.129 h. Moreover, at a given dose, withanoside V, withanolide B, and withanone were detected in plasma; however, the concentration of these constituents was found below LLOQ. Thus, these four major withanoside and withanolides were quantified in plasma supported by ex vivo permeation data exhibiting a time-dependent absorption of withanosides and withanolides across the intestinal barrier. These composite findings provide insights to design a clinical trial of WSE as a potent nutraceutical
International Consensus Statement on Allergy and Rhinology: Rhinosinusitis
No full textIsam Alobid, MD, PhD(1) , Nithin D. Adappa, MD(2) , Henry P. Barham, MD(3) , Thiago Bezerra, MD(4) , Nadieska Caballero, MD(5) , Eugene G. Chang, MD(6) , Gaurav Chawdhary, MD(7) , Philip Chen, MD(8) , John P. Dahl, MD, PhD(9) , Anthony Del Signore, MD(10) , Carrie Flanagan, MD(11) , Daniel N. Frank, PhD(12) , Kai Fruth, MD, PhD(13) , Anne Getz, MD(14) , Samuel Greig, MD(15) , Elisa A. Illing, MD(16) , David W. Jang, MD(17) , Yong Gi Jung, MD(18) , Sammy Khalili, MD, MSc(19) , Cristobal Langdon, MD(20) , Kent Lam, MD(21) , Stella Lee, MD(22) , Seth Lieberman, MD(23) , Patricia Loftus, MD(24) , Luis Macias-Valle, MD(25) , R. Peter Manes, MD(26) , Jill Mazza, MD(27) , Leandra Mfuna, MD(28) , David Morrissey, MD(29) , Sue Jean Mun, MD(30) , Jonathan B. Overdevest, MD, PhD(31) , Jayant M. Pinto, MD(32) , Jain Ravi, MD(33) , Douglas Reh, MD(34) , Peta L. Sacks, MD(35) , Michael H. Saste, MD(36) , John Schneider, MD, MA(37) , Ahmad R. Sedaghat, MD, PhD(38) , Zachary M. Soler, MD(39) , Neville Teo, MD(40) , Kota Wada, MD(41) , Kevin Welch, MD(42) , Troy D. Woodard, MD(43) , Alan Workman(44) , Yi Chen Zhao, MD(45) , David Zopf, MD(46) CONTRIBUTING AUTHOR AFFILIATIONS: (1) Universidad de Barcelona; (2) University of Pennsylvania; (3) Louisiana State University Health Sciences Center; (4) Universidade de São Paulo; (5) ENT Specialists of Illinois; (6) University of Arizona; (7) University of Oxford; (8) University of Texas; (9) University of Indiana; (10) Mount Sinai Beth Israel; (11) Emory University; (12) University of Colorado; (13) Wiesbaden, Germany; (14) University of Colorado; (15) University of Alberta; (16) University of Alabama at Birmingham; (17) Duke University; (18) Sungkyunkwan University; (19) University of Pennsylvania; (20) Universidad de Barcelona; (21) Northwestern University; (22) University of Pittsburgh; (23) New York University; (24) Emory University; (25) University of British Columbia; (26) Yale University School of Medicine; (27) Private Practice; (28) Department of Otolaryngology, Hôtel-Dieu Hospital, Centre de Recherche du Centre Hospitalier de l'Université de Montréal; (29) University of Adelaide; (30) Pusan National University; (31) University of California, San Francisco; (32) University of Chicago; (33) University of Auckland; (34) Johns Hopkins University; (35) University of New South Wales, Australia; (36) Stanford University; (37) Washington University; (38) Harvard Medical School; (39) Medical University of South Carolina; (40) Singapore General Hospital; (41) Taho University; (42) Northwestern University; (43) Cleveland Clinic Foundation; (44) University of Pennsylvania; (45) University of Adelaide; (46) University of Michigan.status: Publishe
Quantitative Determination and Characterization of a Kashmir Saffron (Crocus sativus L.)-Based Botanical Supplement Using Single-Laboratory Validation Study by HPLC-PDA with LC–MS/MS and HPTLC Investigations
No full textFood ingredients
hold a higher nutritional value as a botanical
supplement playing a vital role in modifying and maintaining the physiological
conditions that improve human health benefits. The Kashmir saffron
(Crocus sativus L; KCS) obtained from
dried stigmas is known for its aroma precursors and apocarotenoid
derivatives, imparting a wide range of medicinal values and therapeutic
benefits. In the present study, a simultaneous determination of apocarotenoids
and flavonoids in stigma-based botanical supplements was carried out
using analytical investigations. The high-performance thin-layer chromatography-based
qualitative analysis of the raw material (stigmas, stamens, and tepals)
and stigma extract has been carried out to identify apocarotenoids
and flavonoids. The rapid HPLC-PDA method for the simultaneous quantification
of KCS apocarotenoids was robust, precise (R2 > 0.99), and accurate (80–110%)
as
per the single-laboratory validation data. Furthermore, the combined-expanded
uncertainty (95%; K = 2) was calculated and found
as 0.0035–0.007% (<5.0%) as per the EURACHEM guide for this
HPLC analysis. Additionally, an untargeted identification of 36 compounds
in the botanical supplement was based on the elution order, UV–vis
spectra, mass fragmentation pattern, and standards by ESI-MS/MS analysis
with comprehensive chromatographic fingerprinting. Thus, these analytical
approaches enable a composite profile of the stigma-based extract
as a potential supplement for human health benefits
