96 research outputs found
Design, synthesis and molecular docking of new N-4-piperazinyl ciprofloxacin-triazole hybrids with potential antimicrobial activity
No full textNew N-4-piperazinyl ciprofloxacin-triazole hybrids 6a-o were prepared and characterized. The in vitro antimycobacterial activity revealed that compound 6a experienced promising antimycobacterial activity against Mycobactrium smegmatis compared with the reference isoniazide (INH). Additionally, compound 6a exhibited broad spectrum antibacterial activity against all the tested strains either Gram-positive or Gram-negative bacteria compared with the reference ciprofloxacin. Also, compounds 6g and 6i displayed considerable antifungal activity compared with the reference ketoconazole. DNA cleavage assay of the highly active compounds 6c and 6h showed a good correlation between the Mycobactrium cleaved DNA gyrase assay and their in vitro antimycobactrial activity. Moreover, molecular modeling studies were done for the designed ciprofloxacin derivatives to predict their binding modes towards Topoisomerase II enzyme (PDB: 5bs8)
Applications of well logging techniques to evaluate the groundwater aquifers in the area between southwest Bani Sweif and west Asyoute governorate, upper Egypt
No full textApplications of well logging techniques to evaluate the groundwateraquifers in the area between southwest Bani Sweif and west Asyoute governorate, upper Egypt Safi Eldein.M. Metwally 1, Shimaa. M. Elska 1, *, Fardous. M. Zarif 1, Abdallah. F. Saad 21 Dept. of geophysical exploration, Desert Research Center, Cairo, Egypt 2Dept of Physics., Faculty of Science, Zagazig University, Zagazig, Egypt *Corresponding author: [email protected] AbstractThe relevance of detecting aquifer characterization and aquifer potential has risen with the application of well logging technique as the demand for water has increased. Apart from pumping data, 16 geophysical well logs (resistivity, gamma ray, self-potential, and nuclear logs) are utilized to achieve the main goal of estimating petrophysical parameters (porosity (∅), effective porosity (∅!""), hydraulic conductivity (k), permeability (K) and shale volume
Impact of Implementing Nursing Guidelines on Knowledge and Habits of Patients Receiving Radioactive Iodine
No full textBackground: Radioactive iodine is widely used in nuclear medicine. The radioactive iodine 131 (I-131) is generally utilized in patient with different thyroid disease. Aim of the study: Is to evaluate impact of implementing nursing guidelines on knowledge and habits of patients receiving radioactive iodine. Research design: interventional research design (Pre / post test) was utilized in this study. Setting: This study was conducted in nuclear medicine department at Sohage University Hospital. Sample: 60 patients were included in this study. Tools: Data collected by three tools Interview questionnaire sheet, patient’s knowledge questionnaire sheet and patient habits assessment sheet. Results: there were vital distinction between patients knowledge and habits post implementing nursing guidelines with P value (0.001*). Conclusion: providing nursing guidelines were largely effective on improving patients knowledge and correcting habits. Recommendation: Nurses should be aware by guidelines that given to patients after receiving radioactive iodine and inform patients about them, research should be applied on widely geographical area. Keywords: Nursing Guidelines, knowledge and habits, Radioactive iodine. DOI: 10.7176/JHMN/61-07 Publication date: April 30th 201
Role of endogenous irisin, a novel myokine, in cognitive functions and insulin sensitivity in exercised diabetic rats
No full textEffect of selenium on nutritive value of purslane (Portulaca oleracea L.)
No full textPurslane (Portulaca oleracea) one of the auxiliary plants was traditionally consumed in many parts of the world for its nutritional and medicinal benefits. The nutrient components of purslane such as total protein, total carbohydrates and mineral content such as macro elements (Na, K, Ca and Mg) and micro elements (Fe, Cu, Pb and Zn) were estimated at different concentrations of selenium which treated in soil where the plant cultivated. The protein and carbohydrate contents of leaves as well as protein of stems increase with increasing the selenium concentration, while protein and carbohydrate of roots as well as carbohydrate of stems decrease with increasing Se concentration. The mineral content was also affected by Se concentration, Fe, Cu and Zn of leaves decreased with increasing Se concentration, while K, Ca, Mg and Na are directly proportional with Se concentration. In stems, Zn only is inversely proportional with Se concentration. In roots, Fe, Cu, Mg and K are inversely proportional with Se concentration, while Na, Ca and Zn are directly proportional. The findings of this study revealed that carbohydrates, protein and mineral contents of purslane can be affected and controlled by selenium concentration.
DOI: http://dx.doi.org/10.5281/zenodo.128341
Risk factors for Autistic Spectrum Disorders at Assiut City
No full textAutism is a neurological disorder characterized by qualitative impairments in social interaction, qualitative impairments in communication, and restricted repetitive, stereotyped patterns of behavior, interests, and activities. Autistic Spectrum Disorders are relatively common without known etiology can be found in 80-90% of cases. The aim of this study is to recognize the risk factors for Autistic Spectrum Disorders at Assiut City. The study was descriptive research design; it was conducted in all autism centers at Assiut city which includes 7 centers and included 47 parents who agree to participate in this study selected by convenient sample. The study included a structured interview sheet; this sheet divided into two parts Socio demographic characteristics, and parent’s knowledge about risk factors of Autism. The main findings of the study are: male represent a higher prevalence (72.3%) rate of autism than females (27.7%), and autistic children were more prevalent among families living in urban areas (80.9%) than rural (19.1%). The present study clears that (63.8%) of the fathers and 61.7% of the mothers had university level, the majority (85.1%) of autistic parents’ children hadn’t consanguinity degree while only (14.9%) from them had consanguinity from first degree (cousin), the vast majorities (97.9%) of studied children haven’t family history of autism; the present study found that all mothers of studied children hadn't any history of rubella, diabetes, thyroid and infectious diseases while only (4.3%) were exposed to eclampsia. The study recommended that, increase of public awareness about the risk factors of autism and its prevention by community leaders, mass media, and others especially at rural area. The curriculum of medicine and nursing faculties should include the Autistic Spectrum Disorder (risk factors, diagnosis and different treatment methods). Further studies should be conducted and gained a lot of attention for Autistic children and their parents. Key wards: Autism - Autism Spectrum Disorders – Risk factor
Characterization, expression analysis and RNAi-mediated knockdown of two aquaporin genes in the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae)
No full textAquaporins (AQPs) are integral membrane proteins that can transport water and small molecules across cell membranes in organisms. In a previous report, we identified an AQP (SlAQP1) gene of the cotton leafworm, Spodoptera littoralis. In the current study, we identify and characterize two more SlAQP genes and their developmental and tissues expression. Predicted amino acid sequence and phylogenetic analysis revealed that SlAQP2 is a glycerol channel belonging to the insect specific Eglp subfamily, whereas SlAQP3 is a water specific channel belonging to the Prip subfamily. SlAQP2 expression was detected mainly in the first four larval instars and adult males, whereas SlAQP3 was detected in all developmental stages. SlAQP2 gene expression was mainly detected in larval midgut and Malpighian tubules and adult male testes, whereas SlAQP3 was detected in all the tissues tested. RNAi mediated knockdown of each gene separately resulted in deleterious effects including larval and pupal mortality, deformed pupae and adults and prolonged development. Results were confirmed using qRT-PCR, which revealed downregulation of both genes after injection of larvae with gene specific dsRNA. Our results confirm the presence of Prip and Eglp AQPs in S. littoralis and suggest that Eglp has a role in male reproductive ability
ISOLATION, IDENTIFICATION, MOLECULAR, AND HISTOPATHOLOGICAL INVESTIGATIONS OF TWO PATHOGENIC ENTEROCOCCUS SPECIES FROM TILAPIA IN EGYPTIAN FARMS
No full textAbstract: The rapid increase in global aquaculture have exposed many diseases in aquaculture field, enterococci are one of them. Tilapia is one of the most important and economic fish species in the world. Hundred and thirty diseased farmed Nile tilapia (Oreochromis niloticus) had been investigated for Enterococci collected from different farms in Abbassa, Sahl Elhusseineya, Altal alkabir, Bahr Albaqar, Al Manzalah, Ismailia, Port said during both spring and summer seasons. Two species were identified by automated identification and antibiotic sensitivity testing system (ID&AST) as E. faecalis and E. faecium. In vitro antibiotic susceptibility testing for these two species showed high susceptibility to ampicillin, penicillin, streptomycin, and gentamycin while, E. faecalis was sensitive to vancomycin but E. faecium was resistant to it. These species were confirmed by 16s rRNA as Enterococcus. The two species showed sensitivity and resistance to many antibiotics. Ninety fish with an average weight 50-250 g were exposed to pathogenicity test. Fish were randomly divided into three groups, each contain thirty fish then divided into triplicate (10 fish per replicate). Fish of the first and second groups were intraperitoneally injected with 0.2 ml of bacterial suspension of the 2 species, with comparison of a control group they showed high pathogenicity level (77% for E. faecium and 73% for E. faecalis), also the histopathology revealed vascular congestion and infiltration with inflammatory cells in examined organs tissues (brain, liver, kidney, spleen and heart). In conclusion, E. faecalis and E. faecium were the most common subspecies of Enterococci showing high pathogenicity for Nile tilapia fish in Egypt.Keywords: Identification, Tilapia, Enterococci, Pathogenicity, Histopathology
Loop-mediated isothermal amplification for diagnosis of 18 World Organization for Animal Health (OIE) notifiable viral diseases of ruminants, swine and poultry
No full text- …
