5 research outputs found
Quantitative Estimation of Yeast on Maxillary Denture in Patients with Denture Stomatitis and the Effect of Chlorhexidine Gluconate in Reduction of Yeast
No full textDenture stomatitis is a condition associated with wearing of a denture. The predisposing factor leading to denture stomatitis could be poor oral hygiene, ill-fitting denture and relief areas. Around 30 patients with denture stomatitis were advised to rinse with chlorhexidine gluconate mouthwash for 14 days and were directed to immerse the upper denture in the chlorhexidine solution for 8 hours. The samples were collected by scraping maxillary denture in saline at three intervals, prior to, at the end of 24 hours and after 14 days of treatment, then were inoculated and quantitative estimation of the yeast growth on Sabouraud′s dextrose agar plate was done. It was observed that after a period of 14 days, there was a reduction in the growth of yeast and also improvement in the clinical picture of the oral mucos
Comparative evaluation of debris removal from root canal wall by using EndoVac and conventional needle irrigation: An in vitro study
No full textContext: Microbial control is of paramount importance in Clinical Endodontics. Therefore, cleaning and disinfection of root canals are essential to achieve endodontic success. Aims: The purpose of this study is to compare the efficacy of EndoVac irrigation system and conventional needle (30 gauges side venting needle) irrigation for removal of debris from the root canal walls at coronal, middle and apical third by using the scanning electron microscopy (SEM). Settings and Design: An in vitro randomized control trial study. Materials and Methods: A total of 20 mandibular premolars with completely formed roots were selected and randomly divided into two groups - Group 1: Irrigation with the Conventional system and Group 2: EndoVac irrigation. After access opening and working length determination biomechanical preparation completed up to a rotary protaper F4 file. Groupwise irrigation with sodium hypochlorite and ethylenediaminetetraacetic acid was done with each canal in between instrumentation. Then, the teeth were sectioned in buccolingual direction and the halves were sputter-coated with gold palladium and coronal, middle and apical third were examined by SEM at x2000 magnification. Statistical Analysis: Mann-Whitney test for comparison between methods, Kruskal-Wallis test for comparison among thirds and Miller test for individual comparisons. Results: The apical, middle and cervical root canal thirds were evaluated and the results were analyzed statistically by the Mann-Whitney test for comparison between methods, Kruskal-Wallis test for comparison among thirds and Miller test for individual comparisons. Conclusions: EndoVac group resulted in significantly less debris at apical third compared with the conventional needle irrigation group. There was no statistical significant difference found in debris removal at coronal and middle third of root canal wall between the EndoVac group and conventional needle irrigation group
Colour Stability of Two Different Denture Base Resins and the Efficacy of Two Denture Cleansing Agents after Tea and Coffee Staining: An In-vitro Study
No full textIntroduction: In dentistry, colour is one of the most important dimensions of aesthetics. Due to the diverse food habits in India, stains accumulate on dentures. Various denture cleansing agents such as Fittydent and Clinsodent are widely used, emphasising the importance of patient oral hygiene.
Aim: To evaluate the colour stability of heat-cure activated acrylic denture base resins {Dental Products of India (DPI) and Trevalon} after staining with tea and coffee, subsequently treated with Clinsodent, Fittydent, and distilled water (control group) as cleansing agents.
Materials and Methods: This in-vitro study was conducted at Swargiya Dadasaheb Kalmegh Smruti Dental College and Hospital, Nagpur, in the Department of Prosthodontics, Crown and Bridge, and Implantology in collaboration with Indorama, Nagpur, Maharashtra, India, over a period of approximately 90 days (August-October 2017). Total 120 samples were prepared in-vitro study using custom-made brass metal discs measuring 10 mm by 2 mm. Trevalon and DPI heat-cure acrylic denture base resins were used for sample preparation. Baseline colour stability values were established after immersing the samples in distilled water at 37°C for 24 hours. The samples were then stained for eight hours, followed by immersion in different cleansing agents and distilled water for 12 hours. This process was repeated every 24 hours for 60 days, with the staining and cleansing agents replenished daily. Colour measurements were taken at baseline, 15th, 30th, 45th, and 60th days. Statistical analysis involved t-tests and one-way Analysis of Variance (ANOVA).
Results: The DPI denture base resin exhibited a highly significant ΔE (change in energy) at 45 days (p=0.0001), while Trevalon showed significance at 60 days (p=0.0079). The difference in means was statistically significant using t-tests for independent samples, and the variance-measure analysis indicated statistical significance in the means of ΔE. The comparison of the three cleansing agents at 60 days was statistically obtained using one-way ANOVA.
Conclusion: Dental Products of India (DPI) showed more colour variation than Trevalon. Tea showed a higher staining than coffee. Staining intensity increased over time, peaking at 45 days and stabilising thereafter. Clinsodent is better than Fittydent in removing stains
Student apathy for classroom learning and need of repositioning in present andragogy in Indian dental schools
No full textAbstract Background In the world of technology, when today's student is approaching the on-line /distance learning in the open universities and doing on-line self-assessment, the classroom learning is vanishing slowly. Globally, teachers are taking efforts to improve the pedagogy by implementing effective methods to retain the classroom teaching and student attendance. The present study aims at shedding some light on the need of changing the adult education strategies (andragogy), which can effectively improve the student attendance for lectures. Methods It is an observational study, and the conceptual framework of it is based on beliefs, opinions and personal experiences of the respondents. Triangulation method is used for collecting the data. The data is achieved from three groups of concerned population who could provide valid results to support the study. It is collected by interviewing 10 senior faculty members who are/were the 'education experts' in the universities, while the main concerned groups of present educational stream, i.e. 'institution-teachers' and the 'students', were given questionnaires. 570 teacher respondents and 200 student respondents are the main participants of this study. Results As per data, it has been observed that senior faculty (90%) and students (93.25%) feel need of student motivation more than the institutional teachers (52.44%). P-values were obtained using Chi-Square test for testing the significance of difference between agreement and disagreement for a specific question. Conclusions In India, Universities have already sensed the need of 'teacher development programmes'. But teachers in dental colleges, demand more efforts to be taken by universities and managements in this regard and expect better educational policies to give them accessibility to prove themselves.</p
Rare pathogenic variants in WNK3 cause X-linked intellectual disability
No full textThis is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordData availability: All data are available upon request. The sequence variants in WNK3 (NM_004656.3) reported in the paper have been deposited in ClinVar database. Their respective accession numbers (SCV002107163 to SCV002107168) are indicated in Tables 1 and S1.Purpose
WNK3 kinase (PRKWNK3) has been implicated in the development and function of the brain via its regulation of the cation-chloride cotransporters, but the role of WNK3 in human development is unknown.
Method
We ascertained exome or genome sequences of individuals with rare familial or sporadic forms of intellectual disability (ID).
Results
We identified a total of 6 different maternally-inherited, hemizygous, 3 loss-of-function or 3 pathogenic missense variants (p.Pro204Arg, p.Leu300Ser, p.Glu607Val) in WNK3 in 14 male individuals from 6 unrelated families. Affected individuals had identifier with variable presence of epilepsy and structural brain defects. WNK3 variants cosegregated with the disease in 3 different families with multiple affected individuals. This included 1 large family previously diagnosed with X-linked Prieto syndrome. WNK3 pathogenic missense variants localize to the catalytic domain and impede the inhibitory phosphorylation of the neuronal-specific chloride cotransporter KCC2 at threonine 1007, a site critically regulated during the development of synaptic inhibition.
Conclusion
Pathogenic WNK3 variants cause a rare form of human X-linked identifier with variable epilepsy and structural brain abnormalities and implicate impaired phospho-regulation of KCC2 as a pathogenic mechanism.Estonian Research CouncilNational Natural Science Foundation of ChinaRoyal SocietySouth Carolina Department of Disabilities and Special Needs (SCDDSN)National Institute of Neurological Disorders and Stroke (NINDS
