95 research outputs found

    Carbohydrate binding activity in human spermatozoa: localization, specificity, and involvement in sperm-egg fusion

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    Sperm carbohydrate binding activity is involved in gamete recognition. We identified a human sperm protein extracted under reducing conditions, and with a molecular mass of 65 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and which binds D-mannose coupled to albumin (DMA) in presence of cations and a neutral pH. Epifluorescence microscopy showed that fluorescein-DMA binds to dead or permeabilized sperm heads. The DMA-binding activity of human sperm heads was highly specific for a polysaccharide structure containing charged sugar residues. After capacitation, or induction of the acrosome reaction using solubilized zonae pellucidae, fluorescein-DMA was bound respectively to 10.3% (± 3.5%) and to 37.6% (± 2.1%) of viable sperm heads. The sequential analysis of viable spermatozoa for fluorescein-DMA binding and for rhodamine-Pisum sativum agglutinin binding, showed that DMA-binding sites are present in viable acrosome-reacted spermatozoa. Three dimensional analysis of fluorescence and ultrastructural studies showed that DMA-binding sites are mostly restricted to the sub-acrosomal space of the equatorial segment. Incubation of spermatozoa and zona-free hamster eggs in the presence of DMA was associated with a dose-dependent significant reduction in the number of spermatozoa bound to the oolemma, compared with a control, and to a dose-dependent inhibition of oocyte penetration. This effect was highly specific for DMA, suggesting that DMA-binding sites in human spermatozoa are involved in sperm- egg fusion. -------------------------------------------------------------------------------- Reaxys Database Information

    An early-stage statement-level metric for energy characterization of embedded processors

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    This work presents an early stage statement-level metric for energy characterization of embedded processors. Definition and the framework for metric evaluation are provided. In particular, such a metric is based on an existing assembly-level analysis and some profiling activities performed on a given C benchmark, and it is related to the average energy consumption of a generic C statement, for a given target processor. Its evaluation is performed with a one-time effort and, once available, it can be used to rapidly estimate the energy consumption of a given C function for all the considered processors. Two reference embedded processors are then considered in order to show an example of usage of the proposed metric and framework. (C) 2020 The Author(s). Published by Elsevier B.V

    gCube 4.2.1 - FHNManager Client v. 1.2.0

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    <p> The gCube System - FHNManager Client<br> --------------------------------------------------<br> <br> Client library for federated hosting nodes service<br> <br> <br> This software is part of the gCube Framework (https://www.gcube-system.org/): an<br> open-source software toolkit used for building and operating Hybrid Data<br> Infrastructures enabling the dynamic deployment of Virtual Research Environments<br> by favouring the realisation of reuse oriented policies.<br> <br> The projects leading to this software have received funding from a series of <br> European Union programmes including: <br> * the Sixth Framework Programme for Research and Technological Development - <br> DILIGENT (grant no. 004260); <br> * the Seventh Framework Programme for research, technological development and <br> demonstration - D4Science (grant no. 212488), D4Science-II (grant no. <br> 239019),ENVRI (grant no. 283465), EUBrazilOpenBio (grant no. 288754), iMarine <br> (grant no. 283644); <br> * the H2020 research and innovation programme - BlueBRIDGE (grant no. 675680), <br> EGIEngage (grant no. 654142), ENVRIplus (grant no. 654182), Parthenos (grant <br> no. 654119), SoBigData (grant no. 654024);<br> <br> <br> Version<br> --------------------------------------------------<br> <br> 1.2.0-4.2.1-139969 (2017-02-12)<br> <br> Please see the file named "changelog.xml" in this directory for the release notes.<br> <br> <br> Authors and Maintainers<br> --------------------------------------------------<br> <br> * Nunzio Andrea Galante (nunzioandrea.galante-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Paolo Fabriani (paolo.fabriani-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Gabriele Giammatteo (gabriele.giammatteo-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> <br> <br> Download information<br> --------------------------------------------------<br> <br> Source code is available from SVN: <br> https://svn.research-infrastructures.eu/public/d4science/gcube/trunk/vo-management/fhn-manager-client<br> <br> Binaries can be downloaded from the gCube website: <br> https://www.gcube-system.org/<br> <br> <br> Documentation <br> --------------------------------------------------<br> <br> Documentation is available on-line in the gCube Documentation Wiki:<br> Developer Guide - https://wiki.gcube-system.org/gcube/index.php/<br> User Guide - https://wiki.gcube-system.org/gcube/index.php/<br> Admin Guide - https://wiki.gcube-system.org/gcube/index.php/ <br> <br> Support <br> --------------------------------------------------<br> <br> Bugs and support requests can be reported in the gCube issue tracking tool:<br> https://support.d4science.org/projects/gcube/<br> <br> <br> Installation<br> --------------------------------------------------<br> <br> Please see the file named "INSTALL" in this directory.<br> <br> <br> Licensing<br> --------------------------------------------------<br> <br> This software is licensed under the terms you may find in the file named "LICENSE" in this directory.<br> </p&gt

    gCube 4.1.1 - Federated Hosting Node (FHN) Manager Service v. 1.2.0

    No full text
    <p> The gCube System - Federated Hosting Node (FHN) Manager Service<br> --------------------------------------------------<br> <br> The FHNManger service allows to manage the lifecycle (create, destroy, <br> start, stop and monitor) of Virtual Machines that host gCube nodes <br> on external infrastructures<br> <br> <br> This software is part of the gCube Framework (https://www.gcube-system.org/): an<br> open-source software toolkit used for building and operating Hybrid Data<br> Infrastructures enabling the dynamic deployment of Virtual Research Environments<br> by favouring the realisation of reuse oriented policies.<br> <br> The projects leading to this software have received funding from a series of <br> European Union programmes including: <br> * the Sixth Framework Programme for Research and Technological Development - <br> DILIGENT (grant no. 004260); <br> * the Seventh Framework Programme for research, technological development and <br> demonstration - D4Science (grant no. 212488), D4Science-II (grant no. <br> 239019),ENVRI (grant no. 283465), EUBrazilOpenBio (grant no. 288754), iMarine <br> (grant no. 283644); <br> * the H2020 research and innovation programme - BlueBRIDGE (grant no. 675680), <br> EGIEngage (grant no. 654142), ENVRIplus (grant no. 654182), Parthenos (grant <br> no. 654119), SoBigData (grant no. 654024);<br> <br> <br> Version<br> --------------------------------------------------<br> <br> 1.2.0-4.1.1-133716 (2016-12-15)<br> <br> Please see the file named "changelog.xml" in this directory for the release notes.<br> <br> <br> Authors and Maintainers<br> --------------------------------------------------<br> <br> * Nunzio Andrea Galante (nunzioandrea.galante-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Paolo Fabriani (paolo.fabriani-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Gabriele Giammatteo (gabriele.giammatteo-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> <br> <br> Download information<br> --------------------------------------------------<br> <br> Source code is available from SVN: <br> https://svn.research-infrastructures.eu/public/d4science/gcube/trunk/vo-management/fhn-manager-service<br> <br> Binaries can be downloaded from the gCube website: <br> https://www.gcube-system.org/<br> <br> <br> Documentation <br> --------------------------------------------------<br> <br> Documentation is available on-line in the gCube Documentation Wiki:<br> Developer Guide - https://wiki.gcube-system.org/gcube/index.php/<br> User Guide - https://wiki.gcube-system.org/gcube/index.php/<br> Admin Guide - https://wiki.gcube-system.org/gcube/index.php/ <br> <br> Support <br> --------------------------------------------------<br> <br> Bugs and support requests can be reported in the gCube issue tracking tool:<br> https://support.d4science.org/projects/gcube/<br> <br> <br> Installation<br> --------------------------------------------------<br> <br> Please see the file named "INSTALL" in this directory.<br> <br> <br> Licensing<br> --------------------------------------------------<br> <br> This software is licensed under the terms you may find in the file named "LICENSE" in this directory.<br> </p&gt

    gCube 4.4.0 - Federated Hosting Node (FHN) Manager Service v. 1.2.2

    No full text
    <p> The gCube System - Federated Hosting Node (FHN) Manager Service<br> --------------------------------------------------<br> <br> The FHNManger service allows to manage the lifecycle (create, destroy, <br> start, stop and monitor) of Virtual Machines that host gCube nodes <br> on external infrastructures<br> <br> <br> This software is part of the gCube Framework (https://www.gcube-system.org/): an<br> open-source software toolkit used for building and operating Hybrid Data<br> Infrastructures enabling the dynamic deployment of Virtual Research Environments<br> by favouring the realisation of reuse oriented policies.<br> <br> The projects leading to this software have received funding from a series of <br> European Union programmes including: <br> * the Sixth Framework Programme for Research and Technological Development - <br> DILIGENT (grant no. 004260); <br> * the Seventh Framework Programme for research, technological development and <br> demonstration - D4Science (grant no. 212488), D4Science-II (grant no. <br> 239019),ENVRI (grant no. 283465), EUBrazilOpenBio (grant no. 288754), iMarine <br> (grant no. 283644); <br> * the H2020 research and innovation programme - BlueBRIDGE (grant no. 675680), <br> EGIEngage (grant no. 654142), ENVRIplus (grant no. 654182), Parthenos (grant <br> no. 654119), SoBigData (grant no. 654024);<br> <br> <br> Version<br> --------------------------------------------------<br> <br> 1.2.2-4.4.0-141729 (2017-05-01)<br> <br> Please see the file named "changelog.xml" in this directory for the release notes.<br> <br> <br> Authors and Maintainers<br> --------------------------------------------------<br> <br> * Nunzio Andrea Galante (nunzioandrea.galante-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Paolo Fabriani (paolo.fabriani-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Gabriele Giammatteo (gabriele.giammatteo-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> <br> <br> Download information<br> --------------------------------------------------<br> <br> Source code is available from SVN: <br> https://svn.research-infrastructures.eu/public/d4science/gcube/trunk/vo-management/fhn-manager-service<br> <br> Binaries can be downloaded from the gCube website: <br> https://www.gcube-system.org/<br> <br> <br> Documentation <br> --------------------------------------------------<br> <br> Documentation is available on-line in the gCube Documentation Wiki:<br> Developer Guide - https://wiki.gcube-system.org/gcube/index.php/<br> User Guide - https://wiki.gcube-system.org/gcube/index.php/<br> Admin Guide - https://wiki.gcube-system.org/gcube/index.php/ <br> <br> Support <br> --------------------------------------------------<br> <br> Bugs and support requests can be reported in the gCube issue tracking tool:<br> https://support.d4science.org/projects/gcube/<br> <br> <br> Installation<br> --------------------------------------------------<br> <br> Please see the file named "INSTALL" in this directory.<br> <br> <br> Licensing<br> --------------------------------------------------<br> <br> This software is licensed under the terms you may find in the file named "LICENSE" in this directory.<br> </p&gt

    gCube 4.4.0 - FHNManager Client v. 1.2.0

    No full text
    <p> The gCube System - FHNManager Client<br> --------------------------------------------------<br> <br> Client library for federated hosting nodes service<br> <br> <br> This software is part of the gCube Framework (https://www.gcube-system.org/): an<br> open-source software toolkit used for building and operating Hybrid Data<br> Infrastructures enabling the dynamic deployment of Virtual Research Environments<br> by favouring the realisation of reuse oriented policies.<br> <br> The projects leading to this software have received funding from a series of <br> European Union programmes including: <br> * the Sixth Framework Programme for Research and Technological Development - <br> DILIGENT (grant no. 004260); <br> * the Seventh Framework Programme for research, technological development and <br> demonstration - D4Science (grant no. 212488), D4Science-II (grant no. <br> 239019),ENVRI (grant no. 283465), EUBrazilOpenBio (grant no. 288754), iMarine <br> (grant no. 283644); <br> * the H2020 research and innovation programme - BlueBRIDGE (grant no. 675680), <br> EGIEngage (grant no. 654142), ENVRIplus (grant no. 654182), Parthenos (grant <br> no. 654119), SoBigData (grant no. 654024);<br> <br> <br> Version<br> --------------------------------------------------<br> <br> 1.2.0-4.4.0-139969 (2017-05-01)<br> <br> Please see the file named "changelog.xml" in this directory for the release notes.<br> <br> <br> Authors and Maintainers<br> --------------------------------------------------<br> <br> * Nunzio Andrea Galante (nunzioandrea.galante-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Paolo Fabriani (paolo.fabriani-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Gabriele Giammatteo (gabriele.giammatteo-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> <br> <br> Download information<br> --------------------------------------------------<br> <br> Source code is available from SVN: <br> https://svn.research-infrastructures.eu/public/d4science/gcube/trunk/vo-management/fhn-manager-client<br> <br> Binaries can be downloaded from the gCube website: <br> https://www.gcube-system.org/<br> <br> <br> Documentation <br> --------------------------------------------------<br> <br> Documentation is available on-line in the gCube Documentation Wiki:<br> Developer Guide - https://wiki.gcube-system.org/gcube/index.php/<br> User Guide - https://wiki.gcube-system.org/gcube/index.php/<br> Admin Guide - https://wiki.gcube-system.org/gcube/index.php/ <br> <br> Support <br> --------------------------------------------------<br> <br> Bugs and support requests can be reported in the gCube issue tracking tool:<br> https://support.d4science.org/projects/gcube/<br> <br> <br> Installation<br> --------------------------------------------------<br> <br> Please see the file named "INSTALL" in this directory.<br> <br> <br> Licensing<br> --------------------------------------------------<br> <br> This software is licensed under the terms you may find in the file named "LICENSE" in this directory.<br> </p&gt

    gCube 4.5.0 - Federated Hosting Node (FHN) Manager Service v. 1.2.2

    No full text
    <p> The gCube System - Federated Hosting Node (FHN) Manager Service<br> --------------------------------------------------<br> <br> The FHNManger service allows to manage the lifecycle (create, destroy, <br> start, stop and monitor) of Virtual Machines that host gCube nodes <br> on external infrastructures<br> <br> <br> This software is part of the gCube Framework (https://www.gcube-system.org/): an<br> open-source software toolkit used for building and operating Hybrid Data<br> Infrastructures enabling the dynamic deployment of Virtual Research Environments<br> by favouring the realisation of reuse oriented policies.<br> <br> The projects leading to this software have received funding from a series of<br> European Union programmes including:<br> * the Sixth Framework Programme for Research and Technological Development -<br> DILIGENT (grant no. 004260);<br> * the Seventh Framework Programme for research, technological development and<br> demonstration - D4Science (grant no. 212488), D4Science-II (grant no.<br> 239019),ENVRI (grant no. 283465), EUBrazilOpenBio (grant no. 288754), iMarine<br> (grant no. 283644);<br> * the H2020 research and innovation programme - BlueBRIDGE (grant no. 675680),<br> EGIEngage (grant no. 654142), ENVRIplus (grant no. 654182), Parthenos (grant<br> no. 654119), SoBigData (grant no. 654024);<br> <br> <br> Version<br> --------------------------------------------------<br> <br> 1.2.2-4.5.0-141729 (2017-06-11)<br> <br> Please see the file named "changelog.xml" in this directory for the release notes.<br> <br> <br> Authors and Maintainers<br> --------------------------------------------------<br> <br> * Nunzio Andrea Galante (nunzioandrea.galante-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Paolo Fabriani (paolo.fabriani-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> * Gabriele Giammatteo (gabriele.giammatteo-AT-eng.it), Engineering Ingegneria Informatica S.p.A., IT<br> <br> <br> Download information<br> --------------------------------------------------<br> <br> Source code is available from SVN: <br> https://svn.research-infrastructures.eu/public/d4science/gcube/trunk/vo-management/fhn-manager-service<br> <br> Binaries can be downloaded from the gCube website: <br> https://www.gcube-system.org/<br> <br> <br> Documentation <br> --------------------------------------------------<br> <br> Documentation is available on-line in the gCube Documentation Wiki:<br> Developer Guide - https://wiki.gcube-system.org/gcube/index.php/<br> User Guide - https://wiki.gcube-system.org/gcube/index.php/<br> Admin Guide - https://wiki.gcube-system.org/gcube/index.php/ <br> <br> Support <br> --------------------------------------------------<br> <br> Bugs and support requests can be reported in the gCube issue tracking tool:<br> https://support.d4science.org/projects/gcube/<br> <br> <br> Installation<br> --------------------------------------------------<br> <br> Please see the file named "INSTALL" in this directory.<br> <br> <br> Licensing<br> --------------------------------------------------<br> <br> This software is licensed under the terms you may find in the file named "LICENSE" in this directory.<br> </p&gt
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