19 research outputs found
Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
No full textThis study was aimed to determine the prevalence of Salmonella spp. in foods of animal origin sold at retail stores over the period 2005–2011 in San Luis, Argentina. Characterization of isolates was performed by biochemical and serological tests, antimicrobial susceptibility assays, detection of invA invasion gene by PCR and comparison of genomic profiles by XbaI DNA restriction and PFGE. Twenty seven Salmonella strains were detected in 27 (6.32%) of 427 samples of foods analysed. Sixteen S. Enteritidis and one S. Montevideo strains from chicken meat (17 positive samples/115 total samples), six S. Anatum strains from pork sausages (6/90), two S. Typhimurium strains from liquid egg (2/60) and two S. Montevideo strains from chicken giblets (2/62) were isolated. No Salmonella strains were recovered from chicken carcasses (0/100). Salmonella strains were susceptible to antimicrobials commonly used for clinical treatment. All isolates carried the invA gene. DNA restriction and PFGE analysis revealed similar genomic profiles within each Salmonella serovar regardless of the food type, sampling year, or retail store where samples were purchased, suggesting the possibility of circulation and transmission of clones of limited diversity in our region.Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lazarte Otero, Valeria Sabrina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
An overview of Yersinia enterocolitica and related species in samples of different origin from San Luis, Argentina
No full textThis study is aimed at offering an overview of the prevalence of Yersinia enterocolitica and related species in San Luis, Argentina, from samples of diverse origin received in our laboratory between 1984 and 2014, and providing an analysis of the distribution of Yersinia isolates according to their isolation sources, highlighting bioserotypes and potential reservoirs and vehicles of transmission to humans. From a total of 4572 samples of human, animal, food and environmental origins analyzed by traditional culture methods and molecular techniques, 229 (5%) samples were Yersinia positive. The highest frequency of Yersinia isolates was observed in environmental specimens (14.3%), followed by animal (9.2%), food (5%) and human (0.6%) samples. A total of 255 Yersinia isolates were characterized, including 183 Y. enterocolitica and 72 isolates of other Yersinia species. Biotype 1A associated to several serotypes was identified in Y. enterocolitica isolates from environment (100%), animals (95.5%), foods (71.7%) and human samples (40%); bioserotype 2/O:9 was identified in isolates from foods (25.5%), and biotype 3 was associated with strains from humans (60%), animals (4.5%) and foods (2.8%). This biotype included three strains O:3 and six strains O:5. The data highlight animals and foods as the main Y. enterocolitica sources in our region.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentin
Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
No full textYersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. This study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of Y. enterocolitica strains of different bioserotypes (B/O) isolated from foods and human samples in San Luis, Argentina. Strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, and pulsed-field gel electrophoresis (PFGE). Yersinia enterocolitica was detected in 9.2% of 380 samples, with a distribution of 10.6% (30/284) for food products and 5.2% (5/96) for human samples. Regarding the pathogenic potential, B1A strains of different serotypes were virF– ail–, of which 72.0% (13/18) were ystB+ with virulence-related phenotypic characteristics. Among B2/O:9 isolates, 75.0% (9/12) exhibited the genotype virF+ ail+ ystB– along with phenotypic traits associated with virulence; the same genotype was observed in 80.0% (4/5) of B3/O:3 and B3/O:5 strains. By PFGE, it was possible to separate Y. enterocolitica biotypes into 4 clonal groups (A to D) with 23 genomic types, generating a discriminatory index of 0.96. All isolates were susceptible to antimicrobials used for clinical treatment. This study highlights the presence of pathogenic bioserotypes and the high genomic diversity of the Y. enterocolitica strains isolated in our region.Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Soria, José Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentin
Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
No full textThe prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Escudero, María Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentin
DETECTION AND SURVIVAL OF YERSINIA ENTEROCOLITICA IN GOAT CHEESE PRODUCED IN SAN LUIS, ARGENTINA
No full textDetection limits and the survival of Yersinia enterocolitica in goat cheese were determined by culture and by nested polymerase chain reaction (PCR). Thirty goat cheese samples inoculated with 104 to 101 cfu/g Y. enterocolitica O:9 or O:3 strains were enriched for 0, 3 and 18h in trypticase soy broth (TSB), modified Rappaport broth and a formulated in our laboratory broth (FLB). The lowest detection limits were 1×103cfu/g by culture on Mac Conkey agar after 3h TSB and FLB enrichments, and 1×102cfu/g by nested PCR at 3h from all enrichment broths. Y. enterocolitica survival was studied in 20 goat cheese samples contaminated at levels of 1×106cfu/g and stored at 4° and 22C for 120 days. Y. enterocolitica was detected during 7 and 30 days at 22C and 4C, respectively. Total and fecal coliforms were recovered from microflora of goat cheese, but indigenous Y. enterocolitica was not detected.Fil: Lazarte Otero, Valeria Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentin
Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
No full textYersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42-41,43 (8), B4 O:3 (3) and B1A O:7,8-8-8,19 (2). Autoagglutination (AA), Ca2+-dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA- inv- yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI-PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications: The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.Fil: Mastrodonato, Anna Chiara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Vidal, Roberto. Universidad de Chile; ChileFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentin
Reactivation of a ceparium of Yersinia enterocolitica that had been conserved by two different methods for more than three decades
No full textThe cepariums are genetic resources that preserve microorganisms, guaranteeing the availability of biological material for teaching and scientific research activities. Preservation must guarantee its viability in an inactive pure homogeneous state, under conditions that ensure microscopic, macroscopic, biochemical, physiological, and genetic stability, that is, without phenotypic variations or mutations with respect to the original conditions. The World Federation of Culture Collections recommends creating a duplicate of the collection and storing it in a different location, in case such collections may be lost due to exposure to hazards such as fire, flood, earthquake or war. In addition, it states that they must be preserved using two different methods to ensure conservation. Criteria for method selection are feasibility, purity, cost, amount of culture, and frequency of use. Ultrafreezed and liofilized are long-term preservation methods, also known as methods of choice. Regardless of the preservation techniques used, a quality control must be carried out that includes the evaluation of viability, purity, biochemical and molecular properties. These evaluations must be carried out at the beginning, after the conservation of the first batch, as well as after certain periods of time. The objective of this work was to reactivate 20 strains Yersinia enterocolitica cepariums conserved in the 1980s under two different preservation methods: lyophilization (LIO) and semi-solid medium (SS). The LIO strains were reactivated in tindalized skim milk and cultured at 25 ºC for 24 h, then were replicated in tryptic soy broth (TSB) + 0.6% yeast extract (STBY) and finally in brain heart broth (BHB). The strains conserved in SS medium were reactivated in STBY, picked up at BHB and finally at TSB at 25 ºC for 24 h, respectively. All strains, after reactivated, were seeded on Mac Conkey agar and biochemically identified to corroborate purity. Subsequently, they were ultrafreezed at -80 ° C in TSB + 20% glycerol in duplicate, and in tryptic soy SS medium in duplicate at 4 °C. Counting was not performed because the strains were very weak, and it was necessary to carry out the replicate cultures in nutritionally rich culture medium in order to prioritize survival over quantification. Of the 20 LIO strains, 5 were reactivated, representing 25% survival; meanwhile, from the strains conserved in SS medium, 14 strains could be reactivated, representing 70% survival. This demonstrates the importance of establishing periodic reactivation protocols and control of viability, in order to preserve every strain from the collection. In our study it was shown that conservation in SS medium gives better results than LIO, for long periods of conservation of Y. enterocolitica strains.Fil: Iriarte, Hebe Jorgelina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaLVII SAIB Meeting; XVI SAMIGE MeetingModalidad virtualArgentinaSociedad Argentina de Investigación en Bioquímica y Biología Molecula
Evaluation of the antimicrobial activity of bacteriocins produced by regional Yersinia strains
No full textBacteriocins are extracellular peptides of ribosomal origin and encoded at the plasmid level. Pathogenic Yersinia enterocoliticastrains belonging to biotypes (B) 1 to 5 are cause of gastrointestinal symptoms and immunological sequelae in humans byconsumption of contaminated foods. Y. frederiksenii, Y. intermedia and strains of Y. enterocolitica B1A do not carry thevirulence markers that characterize pathogenic Y. enterocolitica strains; however, they may produce bacteriocins that inhibitthe growth or destroy these pathogenic strains. To contribute to food safety and the human health, the use of bacteria asbiocontrol agents in food has been proposed since they offer safe advantages to the consumer. The aim of this study was toevaluate the antimicrobial activity of bacteriocins produced by Yersinia strains on pathogenic Y. enterocolitica strains isolatedfrom various foods in our region by the plate titration method. Two Y. intermedia B1 (named 79 and 26), one Y. intermediaB6 (10) and three Y. enterocolitica B1A (66, 89, 90) were tested as bacteriocin-producing strains (BPS), and three Y.enterocolitica strains belonging to B2, B3 and B4 were used as indicator strains (IS) of the antimicrobial effect. The spottechnique was performed on a double layer agar. BPS and IS were cultured in Luria Bertani broth (LB) with shaking at 25°Cfor 18 h, and inocula were adjusted to a concentration corresponding to an ODʎ610 0.2. From BPS, two-fold dilutions weremade in LB, and 10 μl of each one was placed on Petri plates with semisolid agar previously inoculated with IS. Plates wereincubated at 25°C and at 10°C for 18 h. The reciprocal of the highest dilution of BPS that produced total inhibition of IS wasconsidered as the titer and expressed in arbitrary units per ml (AU ml -1). Results represent the average of three differentexperiments. At 25°C, the highest inhibition titers were observed for Y. enterocolitica B1A (90) on Y. enterocolitica B2 andB4, with values of 12,800 + 0 AU ml-1and 10,667 + 3,695 AU ml-1(p > 0.05), respectively. At 10 ° C, the highest inhibitiontiters were shown by Y. intermedia B1 (79) on Y. enterocolitica B2 and B4, with values of 9,262 + 3,695 AU ml-1and 6,400+ 0 AU ml-1 (p > 0.05), respectively. All BPS showed lower titers on Y. enterocolitica B3 than on Y. enterocolitica B2 and B4(p < 0.05) at both temperatures. When comparing the two temperatures, most of titles produced by BPS were higher at 25°Cthan at 10°C (p < 0.05). Results obtained in this study demonstrate the capacity of regional Y. intermedia and Y. enterocoliticaB1A strains to produce significant amounts of bacteriocins with inhibitory effect on pathogenic Y. enterocolitica strains, andhighlight the great potential of these substances as antagonists of pathogenic or spoilage bacteria in food, even at refrigerationtemperatures.Fil: Pascual, Lourdes Inés. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis; ArgentinaFil: Iriarte, Hebe Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Mattar Domínguez, María Aída. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Favier, Gabriela Isabel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaReunión Conjunta SAIB-SAMIGE 2021MendozaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Investigaciones en Bioquímic
Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
No full textY. enterocolitica B1A comprise a heterogeneous group of strains that encompasses a wide variety of serotypes. They have been considered non-pathogenic microorganisms due to the lack of plasmid and chromosomal virulence determinants that characterize pathogenic strains; however, strains of this biotype are commonly reported not only from healthy individuals, but also from patients with gastrointestinal disorders. The comprehension of pathogenic mechanisms of B1A strains should focus on certain chromosomal virulence determinants associated to adhesion and invasion in intestinal cells (myfA and ail genes), production of heat-stable enterotoxin (ystB), some proteases (hreP) and iron chelating receptor (fepA), all of them related to growth and survival in host during infection. The synthesis of insecticidal toxins (tccC) is also considered a virulence determinant in Y. enterocolitica B1A. In this work, virulence-associated genes such as ystB (146 bp), myfA (272 bp), hreP (757 bp), fepA (438 bp) and tccC (1035 bp) were studied in 23 local Y. enterocolitica B1A strains of different origins (animal, food, environmental and human clinical samples) by PCR. Strains belonging to serotypes O:5 and O:7,8-8-8,19 (six isolates each), O:41,42-41,43 (four isolates), O:5-4,32-4,33 (three isolates), O:6,30-6,31 (two isolates), O:12,25-12,26 and NA (non-agglutinable/non-determined serotype) (one isolate each) were analyzed. DNA extraction was performed by the ―boiling‖ technique and the amplification products were revealed by agarose gel electrophoresis. The frequency of detection of these genes in decreasing order was: fepA and ystB (22/23), hreP (21/23), tccC (3/23) and myfA (1/23). Regarding the relationship between genes and serotypes, fepA, ystB and hreP genes were demonstrated in strains of all serotypes, meanwhile tccC was observed in O:41,42-41,43 and O:7,8-8-8,19 strains, and myfA was only detected in O:7,8-8-8,19 strains. The serotype O:7,8-8-8,19 was associated to the presence of all genes. Regarding the relationship between genes and strain sources, ystB, hreP and fepA were demonstrated in chicken samples (3 isolates), porcine products (five isolates), ground meat (six isolates), human clinical samples (three isolates), and wild boar, hake fillet and wastewater (one isolate each). The myfA gene was observed in porcine skin (one isolate) and tccC was present in porcine skin (two isolates) and wild boar (one isolate). Interestingly, human samples belonged to serotypes O:5 (two isolates) and O:7,8-8-8,19 (one isolate) showed to be carriers of most of the studied genes, except myfA and tccC. Our results suggest the existence of alternative virulence mechanisms in Y. enterocolitica B1A and that the pathogenic potential of this biotype might be strain-dependent.Fil: Mastrodonato, Anna Chiara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Escudero María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de Cuy
Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina
No full textThe characterization of phenotypic and genotypic virulence markers of Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of virF, ail, ystA, and myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for ystB gene. Four Y. enterocolitica 2/O:9 (bio/serotype) and two 3/O:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFGE and PCR ribotyping, respectively. In both cases the Y. enterocolitica 2/O:9 strains were separately clustered from 1A and 3/O:5 strains. Meat foods might be vehicles of transmission of pathogenic Y. enterocolitica strains in our region.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia de Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lazarte Otero, Valeria Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentin
