1,721,128 research outputs found

    Heavy metals determination by biosensors based on enzyme immobilised by electropolymerisation

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    The work describes the original application of biosensors based on enzyme immobilised by electropolymerisation to heavy metal determination. An inhibition detection scheme has been employed for detecting Hg2+ by an established glucose biosensor based on glucose oxidase immobilised in poly-o-phenylenediamine. The investigated enzymatic inhibition appears reversible and mixed, in agreement with data for the enzyme in solution. A low response time (<2 min) and a rapid recovery of response by EDTA seem the most interesting characteristics of the proposed biosensor at the present stage of development, along with the well known easy preparation of this kind of biosensors. The occurrence of a high response also for Cu2+ opens the possibility to apply the biosensor in total toxic metal content determination

    Itinerario Rosa, Tavola Rotonda sul tema «Quando la tecnologia imita la natura: anche fra macchina e corpo l’integrazione nasce dal dialogo» I progressi della biosensoristica applicata alla medicina.

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    La manifestazione è organizzata ogni anno dal Comune di Lecce per la valorizzazione il ruolo sociale e culturale della donna. https://www.comune.lecce.it/news/2017/02/27/tutti-gli-eventi-in-programma-per-itinerario-rosa La tavola rotonda è stata inserita dalla settimana della cultura scientifica dell'Università del Salent

    Screen-printed glucose oxidase-based biosensor for inhibitive detection of heavy metal ions in a flow injection system

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    A screen-printed electrode amperometric biosensor based on glucose oxidase was successfully used for the inhibitive detection of a wide set of heavy metal ions (Hg2+, Ag+, Cu2+, Cd2+, Co2+, Ni2+) in a flow-injection system. The present work represents a successful way of combining advantages coming from Flow Injection Analysis (FIA) with ones deriving from the use of screen-printing technology in the development of an enzyme inhibition based biosensor. One of the most novel and attractive features of the developed system is the easy and rapid response recovery that does not require any treatment with regenerating reagents. Moreover, sensor response was satisfactory in terms of linear range, limit of detection (LOD), sensitivity and standard deviation. In particular, Hg2+, Cu2+, Cd2+, Co2+, Ni2+ have been successfully revealed at ppm concentration while LOD for Ag+ is in the ppb range. Also the application of the system in glucose detection was satisfactory. Glucose concentrations in the range 0.5–50 mM were detected and sensor response to glucose 10 mM revealed to be stable for 4 hours analysis after being subject to 20 subsequent injections
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