1,721,065 research outputs found
Histological alterations by a diet containing seeds of Garcinia kola: effect on liver, kidney, and intestine in the rat.
No full textHistological examination of liver, kidney, and duodenum of rats fed a diet containing 10% (w/w) dry powdered seeds of Garcinia kola for 6 weeks revealed cellular changes that were not observed in pair-fed control animals. The main cellular changes observed in the rats fed on Garcinia kola diet included vacuolation of duodenal villous epithelial cells, numerous intracytoplasmic vacuoles in hepatocytes, and mild hydropic degeneration in cells of the renal proximal tubular epithelium. These changes are probably due to the flavonoids contained in G. kola seeds
The immunocytochemical expression of various ECM antigens: a useful tool in the evaluation of biocompatibility of implant biomaterials.
Full text linkThe aim of the present work was to investigate the biocompatibility in vitro of biomedical biomaterials employed in stomathology, in order to assess useful biological parameters, i.e. the correlation between cell proliferation rates and the expression of various antigens of the extracellular matrix (ECM), as well as to obtain useful information for the subsequent “in vivo” investigations.. Since in the study of biocompatibility of dental implants many reports have been performed regarding the aspects of osteointegration processes, few studies have examined the relationships between soft tissues and biomaterials [2]. In particular, we would study the relationship between cell proliferation rates of cultured fibroblasts to the immucytochemical expression of molecules involved in cell adhesion mechanisms to ECM, i.e. fibronectin, chondroitin sulfate and alpha5 beta1 integrin. We observed that cell proliferation was related in particular to the expression degree of fibronectin. As far as the different dental implant surfaces were concerned, we found that fibronectin exhibited a greater immunocytochemical expression in fibroblasts cultures in the presence of smooth surfaces correlated with higher fibroblast proliferation rates, suggesting that smooth surfaces could allow a better adhesion of cells of the soft oral tissues, i.e. gingival connective tissue. We think these results could be interesting, since the integration of implant dental materials requires not only the best osteointegration, but also an optimal adhesion of gingival soft tissues to the apical part of the same dental implant. These findings could also suggest that dental implant surfaces should be manufactured to obtain the best osteointegration in its deeper part, whereas the best fibroblast adhesion in its apical portion
The role of Rank-Ligand (RANK-L) and macrophage colony stimulating factor (M-CSF) in differentiating RAW 264,7 TIB 71 cell line towards the osteoclastic phenotype.
No full textCalcium ethylenediaminetetraacetate toxicity in the rat: ultrastructural effects on skin collagen.
No full textThe effect of calcium ethylenediaminetetraacetate (CaEDTA) i.v. infusion on skin ultrastructure was studied in the rat with the aid of transmission electron microscopy. High magnification electron micrographs of collagen fibrils were analysed with a computer aided image analyser, in terms of the Gaussian distribution of fibril diameter and the distance between cross striations of collagen fibrils (D-spacing). CaEDTA caused marked depletion of collagen fibrils in the skin. The collagen fibrils from CaEDTA treated rats exhibited significant increases in D-spacing (about 30%) and diameter (about 40%) compared to saline treated controls. These findings are consistent with earlier published biochemical data indicating that CaEDTA enhances collagen degradation in the rat
A combined approach with rituximab plus anti-TRAIL-R agonistic antibodies for the treatment of haematological malignancies.
No full textMolecular targeted therapies have changed the landscape of cancer research. Agonistic monoclonal antibodies (MoAbs) targeting TRAIL-death receptors (TRAIL-Rs) have been developed and currently used in clinical trials. Binding of such antibodies to TRAIL-R1 and TRAIL-R2 results in death inducing signalling complex (DISC) formation and induction of apoptosis, which represents a natural mechanism of cell growth control and an ideal target for drug development. These novel fully humanized compounds have been associated with conventional chemotherapy in the treatment of advanced solid malignancies, including different types of lymphoma. Here we outline the rationale and potential of a new molecular-based strategy combining agonistic anti-TRAIL-death receptor monoclonal antibodies plus the pioneer of the new biological frontiers of cancer therapy: rituximab
Experimental urethane anaesthesia prevents digoxin intoxication: electrocardiographic and histological study in rabbit.
No full textOsteoclast differentiation from monocyte-macrophage cell line:histochemical and ultrastructural study.
No full textIn vitro models of osteoclastogenesis: morphology and growth characteristics of two RAW 264.7 clones.
No full textSelective distribution of multiple protein kinase C isoforms in mouse cerebellar cortex.
No full textAn immunohistochemical study concerning the distribution of protein kinase C isoforms, a lipid-regulated serine/threonine kinase essential for signal transduction, was performed in mice cerebellar cortex, with particular emphasis on the localization of -iota and -lambda isozymes. By the means of immunoblotting analyses we detected the presence of 11 PKC subspecies in whole cerebellar extracts. Immunoreactivity on cryostat sections revealed, using polyclonal and monoclonal antibodies, that a few isoforms were widely but discretely distributed in all three cortical layers (molecular, granular and Purkinje cells) whereas other isozymes were present in a limited neuronal compartment. Overall, the distribution of several isoforms was in agreement with data obtained by other authors using rat cerebellum. As far as -iota and -lambda isozymes were concerned, we found them abundantly expressed in endothelial cells. Moreover, protein kinase C-lambda was also present in the body of Purkinje cell, conceivably associated with a 200-kDa neurofilament component. In all, these results hint at the possibility that in the cerebellar cortex at least some protein kinase C isoforms are involved in functions other than signal transduction at the synaptic level
Morphological analysis of metastatic potential and antimetastatic drug effects in mice bearing two lines of Lewis lung carcinoma.
No full textTwo lines of Lewis lung carcinoma with a different potential to metastasize spontaneously to the lungs have been examined for their cytological and histological characteristics. Metastatic potential appears to be related with parenchymal organization of the primary tumours, since large haemorrhagic areas containing detached tumour cells and the absence of endothelialized capillaries are observed only in the line with high metastatic potential. At the same time, the cytological characteristics of the cells of the two tumour lines are similar, and do not seem to be related with metastatic potential. After treatment with the selective antimetastatic drugs ICRF-159 and DM-COOK, and with DTIC, the histological appearance of the line with high metastatic potential becomes similar to that of the line with low metastatic potential. These data seem to indicate that the early phases of tumour spread occurring in the primary tumour are of relevance for metastatic potential and control by antimetastatic drugs, and suggest that for DTIC such antimetastatic action may participate to its clinical antitumour effects
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