1,721,132 research outputs found
Expression of neuronal intermediate filament proteins in the rabbit heart conduction tissue: ontogenetic implications
No full textRe: Gold standards for scientists who are conducting animal-based exercise studies.
No full textComment on: J Appl Physiol. 2010 Jan;108(1):219-21
Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.
No full textA novel type of myosin heavy chain (MHC), called 2X, has been recently identified
in type 2 fibers of rat skeletal muscles using an immunochemical approach. In the
present study, the same panel of anti-MHC monoclonal antibodies was used in
immunohistochemistry combined with enzyme histochemistry to identify and compare
type 2X fibers in hindlimb skeletal muscles of rat, mouse, and guinea pig.
Immunohistochemistry shows that 2X MHC is localized in a large subset of type 2
fibers and is co-expressed with 2A or 2B MHC in a small number of fibers. Enzyme
histochemistry shows that type 2X fibers display low myosin ATPase activity after
pre-incubation at pH 4.3 and high activity after paraformaldehyde pre-incubation
at pH 10.4. After pre-incubation at pH 4.6, myosin ATPase shows intermediate and
high activity in rat and mouse 2X fibers, respectively, whereas it is low in
guinea pig 2X fibers. Succinate dehydrogenase displays moderate to high activity
in 2X fibers of all species. Taken together, these staining patterns allow this
novel fiber population to be distinguished from the other type 2 fibers using
only enzyme histochemistry. Nevertheless, the combined use of immuno- and enzyme
histochemistry prevents incorrect fiber typing due to the interspecies
variability of myosin ATPase activity among the correspondent fiber types, and
completely modifies the presently used classification of mouse type 2 fibers
Expression of the glucose regulated protein GRP94 at the cell surface is required for myotube formation
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