1,721,193 research outputs found
Sviluppo di metodologie ed identificazione di parametri biologici indicativi di una ottimale crioconservazione di valvole cardiache.
No full text
Applicazione correlativa del SEM su preparati biologici: alta risoluzione e spettroscopia a dispersione di energia.
No full textHistological and submicroscopic observations on human pineal gland in the course of aging.
No full textIV Colloquium of the European Pineal Study Grou
Electron microscopy localization of NCX1, 2, 3 isoform protein exchangers in neuronal astrocytes.
No full textNa
+
/Ca
2+
exchangers (NCX1, 2 and 3) play relevant role in neural cells, where variations of cytosolic Ca
2+
concentration represent
a pivotal event in many physiological and pathological processes. Astrocytes display a type of excitability based on changes in
intracellular Ca
2+
concentration.
In the present study, electron microscopic immunohistochemistry was applied to investigate the expression of the three NCX1-3
protein isoforms, in astrocytes of cerebral cortex and hippocampus. Results showed that a conspicuous population of astrocytic
cells expressed NCX1–3 in both brain areas. Immunolabeling for NCX1-3 was observed in many glial profiles of various size,
notably in distal astrocytic processes in contiguity of synaptic structures, suggesting the involvement of NCX in shaping astrocytic [Ca]i transients evoked by adjacent synaptic activity. NCX1-3 immunoreactivities (irs) were expressed in astrocytic mitochondria, indicating an important contribution to mitochondrial Ca
2+
regulation in this cell type in situ. In addition, all NCX isoforms were consistently expressed in perivascular astrocytic endfeet, suggesting an important role in regulating the barrier function of blood-brain barrier (BBB). Present immunomorphological work showed that in both brain regions all NCX isoforms were
expressed in astrocytes, thus pointing to a widespread role of the three exchangers in maintaining Ca
2+
homeostasis in glial cells
and suggesting that distinct NCX isoforms may share analogous physiological roles in the brain in vivo
The myotendinous junction plasticity following aerobic exercise.
Full text linkThe myotendinous junction (MTJ) is the site where muscle contractile force is transmitted from the myofibrils across the plasma membrane to the tendon extracellular matrix (ECM), therefore it is a key structure for the locomotor system [1]. In this work, we investigated the relationship between ultrastructural adaptations and the MTJ protein complex modulation after aerobic exercise. In particular, the answer of this anatomical interface to a month of moderate aerobic exercise has been analysed in Sprague-Dawley rats by means of confocal and transmission electron microscopy. Morphological observations confirm the exercise ability to increase the contact area between tissues, increasing the complexity of tendon finger-like processes, which penetrate into the muscle mass. Moreover, these observations suggest a possible MTJ protein complex adaptation after exercise. Confocal images, associated to an immunofluorescence quantification, confirm these ultrastructural observations. Taking together these data reveal that MTJ is a plastic interface. This plasticity can be induced by exercise, which is able to increase the contact area between tissues and to induce a protein synthesis at MTJ level
Il complesso nucleare del poro: struttura e funzione. Microscopia Elettronica
No full textMicroscopia Elettronic
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