99 research outputs found
Capillary-Scale Particle-Beam Liquid Chromatography/Mass Spectrometry Interface: Can Electron Ionization Sustain the Competition?
No full textAnalysis of Thermally Unstable Compounds by a LC/MS Particle Beam Interface with a Modified Ion Source
No full textANAL. CHEM
High-Performance Liquid Chromatography/Mass Spectrometry Methods in Pesticide Analysis
No full textIn this article, a detailed overview of the current high-performance liquid chromatography/mass spectrometry (HPLC/MS) instrumentation, suitable for the determination of a large number of pesticides, is reported. Particular emphasis is given to the role of the mass spectrometric apparatus involved in each interfacing technique with several information regarding the ionization processes, analyzer principles, and system configurations. The article is an up-to-date picture of the current instrumentation available and the reader will find only brief references to some important historical approaches that are no longer in use
Profiling of non-esterified fatty acids in human plasma using liquid chromatography-electron ionization mass spectrometry
No full textThis paper focuses on the development of a
novel approach to analyze underivatized fatty acids in
human plasma. The method is based on liquid–liquid
extraction followed by reversed phase liquid chromatography
coupled to direct-electron ionization mass spectrometry
(LC-Direct-EI-MS). The assay is validated. Calibrations
show satisfactory linearity and precision in the investigated
range of linearity. Recoveries span from 75% to 104%. The
method limits of detection, varying from 0.53 to 5.35 μM,
are satisfactory for the quantitation of non-esterified fatty
acids (NEFAs) in plasma at physiological levels. The
method has been successfully applied to the NEFAs
profiling of plasma samples from healthy adult volunteers
and subjects affected by diabetes mellitus. Compared with
published protocols based on gas chromatography–mass
spectrometry and liquid chromatography coupled to electrospray
ionization mass spectrometry, this method does not
require derivatization and does not show matrix effects,
thus simplifying sample preparation procedure and reducing
the total time of analysis to approximately 90 min. In
addition, Direct-EI-MS allows the acquisition of highquality
NIST library-matchable EI spectra, allowing an
easy-to-obtain identification of the target NEFAs
Enhanced detection sensitivity by large volume injection in reversed-phase micro-high-performance liquid chromatography
No full text
Determination of Acidic and Basic/Neutral Pesticides in Water with a New Microliter Flow Rate LC/MS Particle Beam Interface
No full textLarge-Volume Injection of Acidic Pesticides by Reversed -Phase Micro-High-Performance Liquid Chromatography
No full text- …
