1,726 research outputs found
Circulating small non coding RNAs and microparticles as potential markers of atherosclerotic plaque composition in type 1 diabetes
No full textBackground: Small non coding RNAs (sncRNAs) are endogenous short non coding molecules that regulate gene expression at post-translational level and are involved in several physiopathological processes. Circulating sncRNAs could be found free in biological fluids or loaded into extracellular vesicles, such as microparticles (MPs) in order to reach other tissues and amplify their signal. Next-generation sequencing (NGS) has become the main platform for biological research and biomarker discovery in the profiling of sncRNAs.
Aim: The aim of this study was: 1) to set up a protocol using NGS technology for the identification and quantification of circulating sncRNAs involved in atherosclerotic plaque composition in type 1 diabetic patients (T1DM); 2) to characterize the phenotypes of circulating MPs derived from T1DM, associated with the plaque composition to evaluate the impact of these extracellular vesicles as carrier of specific small non coding RNAs, involved in these pathways.
Material and Methods: Total RNA of 61 T1DM patients with fibrous (CFP; n 30) or calcified (CCP; n 31) carotid plaques was extracted from plasma samples, using a kit for biological fluids. For NGS sequencing, 25 CFP and 26 CCP were evaluated. The preparation of libraries was assessed using the Qiagen system. The sncRNA libraries pool was sequenced through the NGS sequencer MiSeq (Illumina), and the analysis performed by two bioinformatics tools (Partek Flow and CLC Genomics Workbench software). MPs derived from plasma of 40 T1DM patients with fibrous (CFP; n 20) or calcified (CCP; n 20) carotid plaques was assessed by centrifugation (40min x 14,000 rpm a 4°C) and characterized using flow cytometry (CytoFLEX, Beckman Coulter).
Results: An unbiased and accurate sncRNome-wide quantification was obtained, detecting already known circulating sncRNAs (miRNAs, n 2632; piRNAs, n 3286; and tsRNAs, n 640). The bioinformatic analysis using two software on the already known 2632 miRNAs showed a different profile in T1DM with CCP compared to T1DM with CFP. Circulating level of several miRNA implicated in vascular remodeling and glucose metabolism were upregulated in patients with CCP, compared to CFP (miR-503-5p, miR-93-5p, miR-106b-5p and let-7d-5p) and downregulated (miR-451a, miR-10a-5p and miR-29b-3p) in patients with CCP, compared to CFP.
We found that MPs released from endothelial cells and Platelets are enhanced in T1DM with vascular calcification (CCP) compare with T1DM with fibrous plaque (CFP); interestingly, a population of MPs derived from a niche of cells positive for CD34 and α-smooth muscle actin (αSMA) is also increased in CCP. Furthermore, the subgroup of MPs positive for calcification marker was significantly enhanced in patients with CCP in comparison to CFP patients with the main contribution given by CD34+ cells, suggesting a key role of these cells in the development of this vascular complication.
Conclusions: In conclusion, our results demonstrate the power of NGS technology to identify a huge amount of circulating sncRNAs and to discover RNA molecules present in human plasma. The identification of new molecular biomarkers with this ultra-high throughput and sensitive technique (NGS) will help to go further insight specific pathophysiological processes, such as atherosclerotic plaque composition in diabetes, allowing a potentially more targeted therapeutic approach. Furthermore, we demonstrate that microparticles exhibit differential markers in the presence of vascular calcification suggesting a potential role as carrier of small molecules to amplify their signal
Lettera di Alessandra
No full textUn ritratto critico dell'opera di Alessandra Carnaroli, autrice fra le più apprezzate delle ultime generazioni della poesia di ricerca. La sezione a lei dedicata, nel numero della rivista, contiene inoltre saggi di Cecilia Bello Minciacchi, Andrea Cortellessa, e Ivan Schiavone; e vari inediti dell'autrice. Il saggio è pubblicato con lo pseudonimo di Tommaso Ottonieri.A critical portrait of the work of Alessandra Carnaroli, author of the most appreciated in the latest generations of italian research poetry. Published under the pseudonym Tommaso Ottonieri
Electrodermal activity and subjective annoyance in speech recognition tasks with noise as stress indicators
No full textObjectives: Noise can cause changes in the activity of the sympathetic nervous system (SNS), which can be monitored through electrodermal activity (EDA). Our aim was to investigate electrodermal activity and annoyance during speech recognition tasks with competitive noise. Design and study sample: Cross-sectional study with forty normal-hearing adults. The EDA was monitored during relaxation in silence and three recognition tasks with monosyllables, in silence and with competitive pink noise at 65 dBA and 75 dBA. After noise conditions, a subjective scale of annoyance was administered. Results: There were significant differences in the latency of the first EDA signal (p < 0.001), number of EDA peaks (p < 0.001) and percentage of correct answers in the speech recognition task (p < 0.001) when comparing the conditions with and without noise. Regarding the subjective scale, there was a significant difference between the two noise conditions, with higher annoyance scores for the condition with the highest noise level (p < 0.001). Conclusion: Our results suggest that the presence of competitive noise (and higher noise levels) negatively impacts the speech recognition task and increases EDA due to greater activation of the SNS, as well as more intense noise levels causing greater annoyance, possibly resulting from higher levels of stress and listening effort
Selected letters of Alessandra Strozzi
No full textThe letters of Alessandra Strozzi provide a vivid and spirited portrayal of life in fifteenth-century Florence. Among the richest autobiographical materials to survive from the Italian Renaissance, the letters reveal a woman who fought stubbornly to preserve her family's property and position in adverse circumstances, and who was an acute observer of Medicean society. Her letters speak of political and social status, of the concept of honor, and of the harshness of life, including the plague and the loss of children. They are also a guide to Alessandra's inner life over a period of twenty-three years, revealing the pain and sorrow, and, more rarely, the joy and triumph, with which she responded to the events unfolding around her.This edition includes translations, in full or in part, of 35 of the 73 extant letters. The selections carry forward the story of Alessandra's life and illustrate the range of attitudes, concerns, and activities which were characteristic of their author
Challenging the author: Gavin Douglas's Eneados
No full textGavin Douglas’s Eneados, a translation into the “Scottis” tongue of Virgil’s Aeneid, completed in 1513 and first published in London in 1553, presents, as well as the translation of the additional thirteenth book by Maphaeus Vegius, original prologues and marginal notes to the text, rubrics and articulate conclusive material. The present paper analyses this complex paratext as evidence of Douglas’s almost philological attention to the original and his preoccupation with a faithful reproduction; it is also suggested that the models for his organization of the commentary might be both medieval (i.e., manuscripts such as Petrarch’s Virgilius Ambrosianus) and early modern, as in the case of editions of classical works: the most apt example being Jodocus Badius Ascensius’ edition of the Aeneid, printed in 1501. The Eneados thus stands on the threshold between manuscript and print, and might have indicated new possibilities of use of the printing medium in Scotland, and of the value of the translation of a classical text, had history not intervened with the Scottish defeat at Flodden Fields in 1513, which put a temporary stop both to the circulation of the Eneados and to the development of Scottish printing
Ganglioneuroma del surrene: descrizione di un caso clinico.
No full textGANGLIONEUROMA DEL SURRENE: DESCRIZIONE DI UN CASO CLINICO.
Delia Proposito, Francesca De Lucia, Alessandra Giannella, Francesca Frangella, Maria Santangelo, Domenico Magagnano, Giuseppe Pappalardo.
Dipartimento di Chirurgia Generale e Specialistica “Paride Stefanini”.
Sapienza Università di Roma.
OBIETTIVI: Il ganglioneuroma (GN) è il tumore più raro e benigno tra le neoplasie neuroblastiche ed insorge nelle ghiandole surrenali nel 29,7% dei casi. Solitamente asintomatico, questo tumore viene rilevato incidentalmente nella maggior parte dei casi. Il tasso di diagnosi errata di GN alla TC ed alla RMN è del 64,7% e i chirurghi sono spesso poco informati su questa rara patologia. Per questo motivo, abbiamo sottolineato le caratteristiche cliniche, biochimiche e radiologiche del GN del surrene, con l’obiettivo di individuare alcune indicazioni per migliorare la diagnosi pre-operatoria. MATERIALI E METODI: Gli Autori riportano un caso di GN in una donna di 42 anni, giunta alla nostra osservazione per dolori addominali aspecifici. Sottoposta a TC è stata evidenziata una voluminosa neoformazione del surrene destro (14,5 x 11,6 cm.) ed alcune lesioni osteoblastiche a livello della colonna, dello sterno e delle anche. La valutazione completa dell’assetto ormonale è risultata negativa ed ha posto diagnosi di un tumore non funzionante del surrene. Alla PET-TC è stata rilevata un‘attività glucometabolica moderata della massa surrenalica mentre le lesioni ossee sono state considerate metabolicamente non attive. RISULTATI: La paziente è stata sottoposta a surrenectomia dx laparotomica. Il decorso post-operatorio è stato regolare e la paziente è stata dimessa in VII giornata post-operatoria. Ad un follow-up di 6 mesi, è libera da malattia ed è in ottime condizioni cliniche. CONCLUSIONI: Gli Autori sottolineano l’importanza della collaborazione interdisciplinare tra chirurghi, radiologi ed endocrinologi per ottimizzare la gestione clinica e le indicazioni chirurgiche. Per fornire una diagnosi differenziale, sono necessarie un’attenta valutazione degli esami endocrinologici e delle procedure di “imaging”. I chirurghi dovrebbero prendere in considerazione la diagnosi di GN del surrene in caso di: 1) un incidentaloma surrenalico; 2) un tumore non funzionante, che può manifestarsi con sintomi da compressione; 3) una massa capsulata, omogenea, a margini ben definiti, che non determina invasione delle strutture contigue (senza coinvolgimento vascolare), con la presenza di calcificazioni ed attenuazione non enhanced di <40 HU alla TC; 4) una massa surrenalica che alla RMN risulta su T1 ipointensa ed omogenea e su T2 iperintensa ed eterogenea; 5) un livello di “standardized uptake value” alla PET < di 3,0. La surrenectomia laparoscopica è il trattamento di scelta nelle lesioni <6 cm. mentre il ruolo della chirurgia mini-invasiva è controverso in caso di neoformazioni voluminose come nel nostro caso. Il GN del surrene non necessita di chemio o radioterapia adiuvante. Nonostante abbia una prognosi eccellente e le recidive siano rare, si consiglia comunque un follow-up a lungo termine.OBIETTIVI: Il ganglioneuroma (GN) è il tumore più raro e benigno tra le neoplasie neuroblastiche
ed insorge nelle ghiandole surrenali nel 29,7% dei casi. Solitamente asintomatico, questo tumore
viene rilevato incidentalmente nella maggior parte dei casi. Il tasso di diagnosi errata di GN alla TC
ed alla RMN è del 64,7% e i chirurghi sono spesso poco informati su questa rara patologia. Per questo
motivo, abbiamo sottolineato le caratteristiche cliniche, biochimiche e radiologiche del GN del
surrene, con l’obiettivo di individuare alcune indicazioni per migliorare la diagnosi pre-operatoria.
MATERIALI E METODI: Gli Autori riportano un caso di GN in una donna di 42 anni, giunta alla
nostra osservazione per dolori addominali aspecifici. Sottoposta a TC è stata evidenziata una
voluminosa neoformazione del surrene destro (14,5 x 11,6 cm.) ed alcune lesioni osteoblastiche a
livello della colonna, dello sterno e delle anche. La valutazione completa dell’assetto ormonale è
risultata negativa ed ha posto diagnosi di un tumore non funzionante del surrene. Alla PET-TC è stata
rilevata un‘attività glucometabolica moderata della massa surrenalica mentre le lesioni ossee sono
state considerate metabolicamente non attive. RISULTATI: La paziente è stata sottoposta a
surrenectomia dx laparotomica. Il decorso post-operatorio è stato regolare e la paziente è stata
dimessa in VII giornata post-operatoria. Ad un follow-up di 6 mesi, è libera da malattia ed è in ottime
condizioni cliniche. CONCLUSIONI: Gli Autori sottolineano l’importanza della collaborazione
interdisciplinare tra chirurghi, radiologi ed endocrinologi per ottimizzare la gestione clinica e le
indicazioni chirurgiche. Per fornire una diagnosi differenziale, sono necessarie un’attenta valutazione
degli esami endocrinologici e delle procedure di “imaging”. I chirurghi dovrebbero prendere in
considerazione la diagnosi di GN del surrene in caso di: 1) un incidentaloma surrenalico; 2) un tumore
non funzionante, che può manifestarsi con sintomi da compressione; 3) una massa capsulata,
omogenea, a margini ben definiti, che non determina invasione delle strutture contigue (senza
coinvolgimento vascolare), con la presenza di calcificazioni ed attenuazione non enhanced di <40
HU alla TC; 4) una massa surrenalica che alla RMN risulta su T1 ipointensa ed omogenea e su T2
iperintensa ed eterogenea; 5) un livello di “standardized uptake value” alla PET < di 3,0. La
surrenectomia laparoscopica è il trattamento di scelta nelle lesioni <6 cm. mentre il ruolo della
chirurgia mini-invasiva è controverso in caso di neoformazioni voluminose come nel nostro caso. Il
GN del surrene non necessita di chemio o radioterapia adiuvante. Nonostante abbia una prognos
Nicetas Nicaenus, De azymis
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
Polemica scripta anonyma, Dialogus inter Graecum et Cardinales quosdam de processione Spiritus Sancti
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
Theophylactus Bulgariae archiepiscopus, Allocutio ad quemdam ex suis familiaribus de iis quorum Latini incusantur
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
Polemica scripta anonyma, Contra unionem ecclesiarum
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
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